LC-MS and Transcriptome Analysis of Lipopeptide Biosynthesis by Bacillus velezensis CMT-6 Responding to Dissolved Oxygen

Deng, Qi; Lin, Huakuan; Hua, Meifang; Sun, Lijun; Pu, Yuehua; Liao, Jianmeng; Fang, Zhijia; Zhong, Saiyi; Gooneratne, Ravi

doi:10.3390/molecules27206822

Cited by 9 publications

(3 citation statements)

References 36 publications

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“…Surfactin synthesis was accomplished by non-ribosomal peptide synthetase (NRPSs), which mainly involves three steps: the synthesis of fatty acid chain, the activation and loading of fatty acids, and the extension of the peptide chain [51]. Fatty acid synthesis plays as a critical role in lipopeptide synthesis and the gene expression levels are positively correlated with the lipopeptide yield [52,53]. Previous studies have proved that strengthening branched-chain fatty acid biosynthetic pathways was an e cient approach to enhance lipopeptide yield [54].…”

Section: Metabolic Pathways and Regulatory Mechanisms Response For Sp...mentioning

confidence: 99%

The metabolic and physiological responses to spaceflight of a lipopeptide- producing Bacillus subtilis

Qin,

Liu,

Liu

et al. 2024

Preprint

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Background The outer space is an extreme environment that has attracted continuous interest in microbial adaptation and safety, due to its high mutagenesis frequency and genetic variability. To date, several studies have assessed the impact of the space environment on the microbiomes and microorganisms. While the survival of Bacillus subtilis after spaceflight is well established, how the phenotype and metabolic function of B. subtilis respond to space stress is rarely reported. Results In this study, we performed a space flight of the B. subtilis TD7 strain facilitated by the launch project of the Xinyidai Zairen Feichuan-Shiyan Chuan, and compared the strains after spaceflight with the wild-type in terms of their growth, morphology, biofilm formation and secondary metabolism. The spaceflight strain exhibited slower growth, higher cell density, different morphology and decreased biofilm formation. Importantly, a decrease in the lipopeptide production was observed after spaceflight. Thus, we used a multi-omics approach to uncover the molecular mechanisms underlying the changeable secondary metabolism. A total of 14 gene clusters for secondary metabolite biosynthesis were identified in both the wild-type strain and spaceflight strains through whole-genome sequencing, including nonribosomal peptide synthetase. The comparative transcriptome revealed 997 differentially expressed genes which involved in the TCA cycle, fatty acid degradation, amino acid biosynthesis, and quorum sensing systems. The differential expression analysis of 26 lipopeptide-related DEGs further elucidated the relationship between the space environment and the regulation of secondary metabolism. Conclusion Our study is the first study to provide new insight into the behaviors, metabolic functions and adaptation mechanisms of B. subtilis in response to spaceflight. This knowledge could contribute to a better understanding of the relationship between the space environment and microbial adaption mechanisms.

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Section: Metabolic Pathways and Regulatory Mechanisms Response For Sp...mentioning

confidence: 99%

The metabolic and physiological responses to spaceflight of a lipopeptide- producing Bacillus subtilis

Qin,

Liu,

Liu

et al. 2024

Preprint

View full text Add to dashboard Cite

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“…Currently, surfactin is mainly produced by microbial fermentation, but its low yield and high production cost severely limit its industrial production and application [ 9 , 10 ]. The synthesis of surfactin is influenced by the composition of the medium such as carbon and nitrogen sources and culture conditions such as temperature, pH, and dissolved oxygen, and it is important to enhance the yield of surfactin while reducing the cost through scientific and rational optimization of fermentation conditions [ 11 , 12 ]. Among them, the fermentation of inexpensive agricultural and industrial wastes as carbon and nitrogen sources for surfactin production is an effective way to reduce production costs.…”

Section: Prefacementioning

confidence: 99%

Optimization of fermentation conditions for surfactin production by B. subtilis YPS-32

Zhou

Yang

Li³

et al. 2023

BMC Microbiol

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Background Surfactin produced by microbial fermentation has attracted increasing attention because of its low toxicity and excellent antibacterial activity. However, its application is greatly limited by high production costs and low yield. Therefore, it is important to produce surfactin efficiently while reducing the cost. In this study, B. subtilis strain YPS-32 was used as a fermentative strain for the production of surfactin, and the medium and culture conditions for the fermentation of B. subtilis YPS-32 for surfactin production were optimized. Results First, Landy 1 medium was screened as the basal medium for surfactin production by B. subtilis strain YPS-32. Then, using single-factor optimization, the optimal carbon source for surfactin production by B. subtilis YPS-32 strain was determined to be molasses, nitrogen sources were glutamic acid and soybean meal, and inorganic salts were KCl, K2HPO4, MgSO4, and Fe2(SO4)3. Subsequently, using Plackett-Burman design, MgSO4, time (h) and temperature (°C) were identified as the main effect factors. Finally, Box-Behnken design were performed on the main effect factors to obtain optimal fermentation conditions: temperature of 42.9 °C, time of 42.8 h, MgSO4 = 0.4 g·L− 1. This modified Landy medium was predicted to be an optimal fermentation medium: molasses 20 g·L− 1, glutamic acid 15 g·L− 1, soybean meal 4.5 g·L− 1, KCl 0.375 g·L− 1, K2HPO4 0.5 g·L− 1, Fe2(SO4)3 1.725 mg·L− 1, MgSO4 0.4 g·L− 1. Using the modified Landy medium, the yield of surfactin reached 1.82 g·L− 1 at pH 5.0, 42.9 ℃, and 2% inoculum for 42.8 h, which was 2.27-fold higher than that of the Landy 1 medium in shake flask fermentation. Additionally, under these optimal process conditions, further fermentation was carried out at the 5 L fermenter level by foam reflux method, and at 42.8 h of fermentation, surfactin reached a maximum yield of 2.39 g·L− 1, which was 2.96-fold higher than that of the Landy 1 medium in 5 L fermenter. Conclusion In this study, the fermentation process of surfactin production by B. subtilis YPS-32 was improved by using a combination of single-factor tests and response surface methodology for test optimization, which laid the foundation for its industrial development and application.

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“…Currently, surfactin is mainly produced by microbial fermentation, but its low yield and high production cost severely limit its industrial production and application [9,10]. The synthesis of surfactin is in uenced by the composition of the medium such as carbon and nitrogen sources and culture conditions such as temperature, pH, and dissolved oxygen, and it is important to enhance the yield of surfactin while reducing the cost through scienti c and rational optimization of fermentation conditions [11,12]. Among them, the fermentation of inexpensive agricultural and industrial wastes as carbon and nitrogen sources for surfactin production is an effective way to reduce production costs.…”

Section: Prefacementioning

confidence: 99%

Optimization of fermentation conditions for surfactin production by B. subtilis YPS-32

Zhou

Yang

Peng

et al. 2023

Preprint

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BACKGROUND Surfactin produced by microbial fermentation has attracted increasing attention because of its low toxicity and excellent antibacterial activity. However, its application is greatly limited by high production costs and low yield. Therefore, it is important to produce surfactin efficiently while reducing the cost. In this study, B. subtilis strain YPS-32 was used as a fermentative strain for the production of surfactin, and the medium and culture conditions for the fermentation of B. subtilis YPS-32 for surfactin production were optimized. RESULTS First, Landy 1 medium was screened as the basal medium for surfactin production by B. subtilis strain YPS-32. Then, using single-factor optimization, the optimal carbon source for surfactin production by B. subtilis YPS-32 strain was determined to be molasses, nitrogen sources were glutamic acid and soybean meal, and inorganic salts were KCl, K2HPO4, MgSO4, and Fe2(SO4)3. Subsequently, using Plackett-Burman design, MgSO4, time (h) and temperature (°C) were identified as the main effect factors. Finally, Box-Behnken design were performed on the main effect factors to obtain optimal fermentation conditions: temperature of 42.9°C, time of 42.8 h, MgSO4 = 0.4 g·L− 1. This modified Landy medium was predicted to be an optimal fermentation medium: molasses 20 g·L− 1, glutamic acid 15 g·L− 1, soybean meal 4.5 g·L− 1, KCl 0.375 g·L− 1, K2HPO4 0.5 g·L− 1, Fe2(SO4)3 1.725 mg·L− 1, MgSO4 0.4 g·L− 1. Using the optimized medium, the yield of surfactin reached 1.82 g·L− 1 at pH 5.0, 42.9℃, and 2% inoculum for 42.8 h, which was 2.27-fold higher than before optimization. Additionally, under these optimal process conditions, further fermentation was carried out at the 5 L fermenter level by foam reflux method, and at 42.8 h of fermentation, surfactin reached a maximum yield of 2.39 g·L− 1, which was 2.96-fold higher than before optimization. Conclusion In this study, the fermentation process of surfactin production by B. subtilis YPS-32 was improved by using a combination of single-factor tests and response surface methodology for test optimization, which laid the foundation for its industrial development and application.

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LC-MS and Transcriptome Analysis of Lipopeptide Biosynthesis by Bacillus velezensis CMT-6 Responding to Dissolved Oxygen

Cited by 9 publications

References 36 publications

The metabolic and physiological responses to spaceflight of a lipopeptide- producing Bacillus subtilis

The metabolic and physiological responses to spaceflight of a lipopeptide- producing Bacillus subtilis

Optimization of fermentation conditions for surfactin production by B. subtilis YPS-32

Optimization of fermentation conditions for surfactin production by B. subtilis YPS-32

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