LC–MS/MS and GC–MS based phytochemical perspectives and antimicrobial effects of endophytic fungus Chaetomium ovatoascomatis isolated from Euphorbia milii

Shaker, Kamel H.; Zohair, Moustafa M.; Hassan, A.; Sweelam, Heba-tollah M.; Ashour, Warda E.

doi:10.1007/s00203-022-03262-5

Cited by 15 publications

(4 citation statements)

References 52 publications

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“…According to the reported procedures [ 16 ], GC-MS analysis was conducted utilizing a Thermo Scientific Trace GC Ultra/ISQ Single Quadrupole MS instrument, equipped with a TG-5MS fused silica capillary column of 30 m in length, 0.251 mm in diameter, and featuring a 0.1 mm film thickness. The experimental setup involved the utilization of an electron ionization system with an ionization energy of 70 electron volts (eV).…”

Section: Methodsmentioning

confidence: 99%

Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21

Hamed,

Ghareeb,

Kelany

et al. 2024

BMC Biotechnol

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The growing spread of infectious diseases has become a potential global health threat to human beings. According to WHO reports, in this study, we investigated the impact of co-cultivating the isolated endophytic fungus Aspergillus sp. CO2 and Bacillus sp. COBZ21 as a method to stimulate the production of natural bioactive substances. (GC/MS)-based metabolomics profiling of two sponge-associated microbes, Aspergillus sp. CO2 and Bacillus sp. COBZ21, revealed that the co-culture of these two isolates induced the accumulation of metabolites that were not traced in their axenic cultures. By detection of different activities of extracts of Bacillus sp. COBZ21 and Aspergillus sp. CO2 and coculture between Bacillus sp. COBZ21 and Aspergillus sp. CO2. It was noted that the coculture strategy was the reason for a notable increase in some different activities, such as the antimicrobial activity, which showed potent activity against Escherichia coli ATCC 25,922, Staphylococcus aureus NRRLB-767, and Candida albicans ATCC 10,231. The antibiofilm activity showed significant biofilm inhibitory activity toward Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 10,145, and Staph aureus NRRLB-767, with activity up to 53.66, 71.17, and 47.89%, while it showed low activity against E. coli ATCC 25,922, while the antioxidant activity based on the DPPH assay showed maximum activity (75.25%). GC-MS investigations revealed the presence of variable chemical constituents belonging to different chemical categories, which reflected their chemical diversity. The main components are (+-) cis-Deethylburnamine (2.66%), Bis(3,6,9,12-tetraoxapentaethylene) crowno-N,N,N’,N’-tetra methylpphanediamine (2.48%), and 11-phenyl-2,4,6,8-tetra(2-thienyl)-11-aza-5,13-dithiaeteracyclo[7.3.0.1(2,8)0.0(3,7)] trideca-3,6-diene-10,12,13-trione (3.13%), respectively, for Bacillus sp. axenic culture, Aspergillus sp. CO2, Aspergillus sp. CO2, and Bacillus sp. COBZ21 coculture. By studying the ADME-related physicochemical properties of coculture extract, the compound showed log Po/w values above 5 (8.82). The solubility of the substance was moderate. In order to provide a comprehensive definition of medicinal chemistry and leadlikness, it is important to note that the latter did not meet the criteria outlined in the rule of three (RO3). The toxicity prediction of the coculture extract was performed using the ProTox II web server, which showed that the selected compound has no pronounced toxicity.

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Section: Methodsmentioning

confidence: 99%

Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21

Hamed,

Ghareeb,

Kelany

et al. 2024

BMC Biotechnol

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“…The major compounds with bio-active properties detected are (1) L-serine-O-sulfate, (2) Se-methyl-L-selenocysteine, (3) 3,5-dibromo-L-tyrosine, (4) quintozene, (5) doxorubicin, (6) estramustine, (7) disulfiram, (8) xylitol, (9) dodecanedioic acid, (10) 6-endo-hydroxycineole, (11) 10-formyldihydrofolate, (12) 5,10-methylenetetrahydrofolate, (13) prunasin, (14) linustatin, (15) delphinidin, ( 16) Seadenosylselenomethionine, and ( 17) naringenin (Table S4). Similarly, the endophytic fungus Chaetomium ovaoascomatis produced various metabolites such as grevilline B, austdiol, quinalizatrine, and questinol, and is a viable candidate for drug development [38].…”

Section: Chemical Profiling Of Cladosporium Cladosporioides (Mcs2) Fu...mentioning

confidence: 99%

Diversity, Antimicrobial, Antioxidant, and Anticancer Activity of Culturable Fungal Endophyte Communities in Cordia dichotoma

Sharma,

Bharti,

Goswami

et al. 2023

Molecules

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Endophytic fungi are a significant source of secondary metabolites, which are chemical compounds with biological activities. The present study emphasizes the first-time isolation and identification of such fungi and their pharmacological activities from the medicinal plant Cordia dichotoma, which is native to Jammu, India. The Shannon Wiener diversity index revealed a wide range of fungal endophytes in root (1.992), stem (1.645), and leaf (1.46) tissues. A total of 19 endophytic fungi belonging to nine different genera were isolated from this plant and the majority belonged to the Ascomycota phylum. ITS rRNA gene sequencing was used to identify the fungal strains and they were submitted in NCBI GenBank. The most potent fungal isolate Cladosporium cladosporioides OP870014 had strong antimicrobial, antioxidant, and anticancer activity against MCF-7, HCT-116, and PC-3 cancer cell lines. The LC-MS and GC-MS analyses of the ethyl acetate extract of C. cladosporioides were examined to identify the bioactive metabolites. The major compounds of the crude extract derived from C. cladosporioides OP870014, according to GC-MS, are spiculisporic acid; dibutyl phthalate; phenylethyl alcohol; cyclohexanone, 2,3,3-trimethyl-2-3-methylbutyl; pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(phenylmethyl);2,5-piperazinedione,3,6-bis(2-methylpropyl); and heneicosane which possessed antimicrobial, anticancerous, and antioxidant activities. The findings revealed that C. dichotoma has the capacity to host a wide variety of fungal endophytes and that secondary metabolites from the endophytic fungus may be a source of alternative naturally occurring antimicrobial, antioxidant, and cytotoxic compounds.

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“…Traditional antibiotics are becoming less effective, which requires a change in the approach to microbial infections. In this context, there is a possibility that bioactive compounds from endophytic fungi can serve as new antimicrobial agents with significant potential [63]. These compounds, ranging from alkaloids to terpenoids, have the remarkable capacity to inhibit the growth and proliferation of a diverse spectrum of pathogens [64].…”

Section: Antimicrobial Propertiesmentioning

confidence: 99%

Health-Promoting Effects of Bioactive Compounds from Plant Endophytic Fungi

Wijesekara,

2023

JoF

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The study examines the intricate relationship between plants and the endophytic fungi inhabiting their tissues. These fungi harmoniously coexist with plants, forming a distinct symbiotic connection that has caught scientific attention due to its potential implications for plant health and growth. The diverse range of bioactive compounds produced by these fungi holds significant promise for human health. The review covers various aspects of this topic, starting by introducing endophytic microorganisms, explaining their colonization of different plant parts, and illuminating their potential roles in enhancing plant defense against diseases and promoting growth. The review emphasizes the widespread occurrence and diversity of these microorganisms among plant species while highlighting the complexities and significance of isolating and extracting bioactive compounds from them. It focuses on the health benefits of these bioactive compounds, including their capacity to exhibit antioxidant, anti-inflammatory, antimicrobial, and anticancer effects. The review delves into the mechanisms behind these health-promoting effects, spotlighting how the compounds interact with cellular receptors, signaling pathways, and gene expression. In conclusion, the review provides a comprehensive overview of health-promoting bioactive compounds from plant endophytic fungi. It outlines their multifaceted impact, potential applications, and future research avenues in health and medicine.

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LC–MS/MS and GC–MS based phytochemical perspectives and antimicrobial effects of endophytic fungus Chaetomium ovatoascomatis isolated from Euphorbia milii

Cited by 15 publications

References 52 publications

Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21

Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21

Diversity, Antimicrobial, Antioxidant, and Anticancer Activity of Culturable Fungal Endophyte Communities in Cordia dichotoma

Health-Promoting Effects of Bioactive Compounds from Plant Endophytic Fungi

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