LC-MS/MS Bioanalytical Method for Simultaneous Quantification of Three H1-Antihistaminic Drugs in Human Plasma

Abstract

The aim of the study was to validate a bioanalytical method, for quantifying desloratadine, loratadine and cetirizine in biological samples, useful in toxicological studies. The analytical performance was investigated using the following parameters: selectivity, limit of detection and quantification, linearity, precision, accuracy, matrix effect and analytical stability in the biological matrix. The method optimization was performed for the LC-MS/MS Ultimate 3000 instrument provided by Thermo Fisher Scientific. Keywords: H1-antihistaminic drugs, human plasma, LC-MS/MS