LC-MS/MS method for the confirmatory determination of aromatic amines and its application in textile analysis

Abstract

A confirmation method for the determination of 18 aromatic amines originating from azo dyes after reductive cleavage was developed. The method is based on the use of high-performance liquid chromatography/tandem mass spectrometry with atmospheric-pressure chemical ionization. For the identification of the analytes one precursor ion and two daughter ions (multi-reaction monitoring, MRM) were selected and the LC-MS/MS parameters optimized to obtain high sensitivity and selectivity. The linear ranges varied from 0.1-1 to 30-50 microg mL(-1) with correlation coefficients of 0.99 or better. The applicability of the method to determine o-tolidine (3,3'-dimethylbenzidine) and 3,3'-dimethoxybenzidine in textiles following reductive cleavage of acid red 114, trypan blue, and Chicago sky blue 6B was demonstrated.

The (Un)Certainty of Selectivity in Liquid Chromatography Tandem Mass Spectrometry

“…Database B contains product ion spectra data and is constructed from the product ion spectra of 3629 compounds obtained from our own research, the Massbank database [23] and literature [24][25][26][27][28][29][30][31]. Of 3629 compounds, the positive ion mode electrospray ionization collision-induced dissociation (CID) MS spectra were converted to binary spectra with a cut-off level at 5 % relative intensity and thus information on ion abundances was removed.…”

Ultrahigh‐performance liquid chromatography/electrospray ionization linear ion trap Orbitrap mass spectrometry of antioxidants (amines and phenols) applied in lubricant engineering

“…UHPLC/HRMS-LITMS 2 chromatograms of the separation of twelve phenolic compounds measured in negative ion mode. Accurate mass full scan chromatogram at a resolution of 30 000 (FWHM) at the top, followed by the LITMS 2 of the preselected precursor ions of compounds 1,2,5,8,6,4,11,12,13,9,14 The LOD is not distinguishable from the corresponding lower LOQ for the measured serial dilution.…”

“…Earlier LC/ESI‐MS methods have shown that good separation and detection of aromatic amines and phenols are achievable, at least in simple matrices such as powdered dyes or solid polymers . On the other hand, LC/ESI‐MS can also be applied for the analysis of samples dissolved in hydrophobic matrices as was shown for the determination of degradation products of ester‐based lubricants …”

Studies on an efficient method for determining 3,3’-dimethylbenzidine in the workplace air

“…W dostępnym piśmiennictwie można znaleźć metody oznaczania 3,3'-dimetylobenzydyny w próbkach środowiskowych (woda), płynach ustrojowych człowie-ka (mocz) i w barwnikach [8][9][10].…”