LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients

Ouhibi, Salma; Vidal, Arnau; Martins, Carla; Gali, Ridha; Hedhili, Abderrazzek; Saeger, Sarah De; Boevre, Marthe De

doi:10.1016/j.fct.2019.110994

Cited by 22 publications

(38 citation statements)

References 57 publications

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“…Several methods can be applied in order to reduce this variability [52]. Among them, the normalization of the mycotoxin levels for creatinine concentration (µg mycotoxin L -1 urine/g creatinine L −1 urine) is one of the most employed [30,53]. However, this procedure also has some drawbacks, since it is not clear if the mycotoxin/creatinine ratio can be used for interindividual comparison.…”

Section: Figurementioning

confidence: 99%

“…However, when very polar mycotoxins (such as PAT or FBs) are in the samples, high levels of organic solvent during the extraction procedure (that contributes to less polar mycotoxin extraction) lead to low recovery values, probably because they remain in the discarded aqueous phase during the extraction [67]. In these cases, a high percentage of water is needed [53] and less polar mycotoxins can be non-extracted. In the case of The most widely used mycotoxin-related extraction, cleanup and enrichment procedure was solid-phase extraction (SPE), based on the retention of analytes on a fixed support in a cartridge.…”

Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

“…The introduction of Ultra-LC (UHPLC), characterized by uniform column packing material with a particle size of less than 2-3 µm and new pumps and detectors, has represented an improvement, given that it achieves shorter run times, reduces solvent consumption and improves chromatographic resolution and efficiency. UHPLC was reported in 42.8% of the chromatographic methods summarized in this review [48,53,58,64,65,68,69,[81][82][83].…”

Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

“…Though validation is mandatory [106], there are no guidelines for the validation of mycotoxin quantification methods in human body fluids. Some authors [53,64,65] referred to EU Commission Decision No. 2002/657/EC [107], which mentions the rules for the analytical methods to be used in the testing of official samples (residues in live animals and animal products), or the EU Commission Regulation (EC) No.…”

Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

“…To date, only a few methods have been developed for multi-analyte detection of mycotoxins in plasma and serum samples [48,53,[58][59][60]64,65,82,111], and 90% of these methods have been developed in the last three years. Only five (50%) of these studies proposed methods for detecting more than 10 mycotoxins simultaneously.…”

Section: Multi-biomarker Studiesmentioning

confidence: 99%

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Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Arce-López

Lizarraga

Vettorazzi

et al. 2020

Toxins

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This manuscript reviews the state-of-the-art regarding human biological monitoring (HBM) of mycotoxins in plasma, serum and blood samples. After a comprehensive and systematic literature review, with a focus on the last five years, several aspects were analyzed and summarized: (a) the biomarkers analyzed and their encountered levels, (b) the analytical methodologies developed and (c) the relationship between biomarker levels and some illnesses. In the literature reviewed, aflatoxin B1-lysine (AFB1-lys) and ochratoxin A (OTA) in plasma and serum were the most widely studied mycotoxin biomarkers for HBM. Regarding analytical methodologies, a clear increase in the development of methods for the simultaneous determination of multiple mycotoxins has been observed. For this purpose, the use of liquid chromatography (LC) methodologies, especially when coupled with tandem mass spectrometry (MS/MS) or high resolution mass spectrometry (HRMS) has grown. A high percentage of the samples analyzed for OTA or aflatoxin B1 (mostly as AFB1-lys) in the reviewed papers were positive, demonstrating human exposure to mycotoxins. This review confirms the importance of mycotoxin human biomonitoring and highlights the important challenges that should be faced, such as the inclusion of other mycotoxins in HBM programs, the need to increase knowledge of mycotoxin metabolism and toxicokinetics, and the need for reference materials and new methodologies for treating samples. In addition, guidelines are required for analytical method validation, as well as equations to establish the relationship between human fluid levels and mycotoxin intake.

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Section: Figurementioning

confidence: 99%

Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

Section: Mycotoxin Determination In Human Blood Plasma and Serummentioning

confidence: 99%

Section: Multi-biomarker Studiesmentioning

confidence: 99%

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Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Arce-López

Lizarraga

Vettorazzi

et al. 2020

Toxins

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Mycotoxins

Luciano‐Rosario,

Keller

2023

ClinMicroNow

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Mycotoxins are secondary metabolites produced by many different species of ubiquitous fungi that have adverse effects on humans and animals. This chapter reviews the most common mycotoxins and their relevance to sick building syndrome, bioterrorism, and food safety. It briefly addresses the impact of mycotoxins on animal and human health. The aflatoxin class of mycotoxins was the first to be discovered and studied. Citrinin is a simple, low‐molecular‐weight compound that crystallizes as light yellow needles. Cyclopiazonic acid is an indole‐tetramic acid mycotoxin produced by several species of Penicillium and Aspergillus . Fumonisins are among the most recently discovered mycotoxins. Zearalenone is a common contaminant of cereal crops worldwide and has been implicated in hyperestrogenism of farm animals as a result of digestion of moldy corn and grains.

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Biomonitoring of ochratoxin A, 2′R-ochratoxin A and citrinin in human blood serum from Switzerland

et al. 2022

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Biomonitoring of mycotoxins and their metabolites in biological fluids is increasingly used to assess human exposure. In this study, biomarkers of ochratoxin A (OTA) and citrinin (CIT) exposure were determined in a large number of serum samples from healthy blood donors in Switzerland. In 2019, 700 samples from different regions were obtained. From 240 donors, a second sample (taken 2–9 months later) was available for analysis. Moreover, 355 blood donor samples from 2005 from all regions in Switzerland and 151 additional samples from the southern Swiss region of Ticino from 2005 could be analysed.OTA, 2′R-ochratoxin A (2′R-OTA), ochratoxin alpha (OTα), CIT and dihydrocitrinone (DH-CIT) were analysed using validated targeted methods including precipitation and online SPE clean-up.OTA and 2′R-OTA were frequently detected (OTA in 99%; 2′R-OTA in 51% of the tested samples). The mean concentration in all positive samples was 0.4 ng/mL for OTA and 0.2 ng/mL for 2′R-OTA. OTα was not detected in any sample above the limit of quantification (LOQ). In contrast to OTA, CIT and DH-CIT were only quantifiable in 2% and 0.1% of the samples, respectively. No significant trend was observed between the samples from 2005 and the more recent samples, but OTA concentrations were usually higher in serum samples from the southern Swiss region of Ticino and in males compared to females.Our extensive data fit well within the framework of previously published values for the healthy adult European population.

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LC-MS/MS methodology for simultaneous determination of patulin and citrinin in urine and plasma applied to a pilot study in colorectal cancer patients

Cited by 22 publications

References 57 publications

Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Human Biomonitoring of Mycotoxins in Blood, Plasma and Serum in Recent Years: A Review

Mycotoxins

Biomonitoring of ochratoxin A, 2′R-ochratoxin A and citrinin in human blood serum from Switzerland

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