LC–MS/MS screening strategy for cannabinoids, opiates, amphetamines, cocaine, benzodiazepines and methadone in human serum, urine and post-mortem blood as an effective alternative to immunoassay based methods applied in forensic toxicology for preliminary examination

A fast and simple approach for the quantification of 40 illicit drugs, medicines, and pesticides in blood and urine samples by UHPLC‐MS/MS

“…The calibration curves for each analytical in blood were determined taking into account, where possible, the therapeutic and toxic concentrations of each substance. The concentration ranges in blood determined in this study are in agreement with those reported by Dziadosz et al that stipulated values between 2 and 50 ng mL −1 . Arora et al used working concentrations between 7.8 and 250 ng mL −1 .…”

“…These results were considered more significant than the values reported by Steuer et al 29 and Sempio et al 37 In our study, we determined that LLOQs for whole blood ranged from 0.5 to 10 ng mL −1 for benzodiazepines and antidepressants, while Mut et al 38 reported in recent publications using the techniques of GC-MS and LC-MS. 25,29,30 The calibration curves for each analytical in blood were determined taking into account, where possible, the therapeutic and toxic concentrations of each substance. The concentration ranges in blood determined in this study are in agreement with those reported by Dziadosz et al 39 that stipulated values between 2 and 50 ng mL −1 .…”

“…From the latter, benzodiazepines were detected in 50% of the samples and, at least one analyte of cocaine has been detected in 23% of the samples. This extraction procedure was preferred over that described by Hegstad et al, Sempio et al, and Dziadosz et al because the present study features an approach of sample preparation and the extensive variety of substances in multi‐analyte screening, including the pesticides, that has not been reported in recently published papers.…”

Postmortem findings of pipamperone after fatal intoxications and its distribution in body fluids and tissues

“…After shaking (15 minutes) and centrifugation (5 minutes), the organic layer was mixed with 10 μL 2‐propanol solution with 0.1 mol/L HCl and was evaporated to dryness under a stream of nitrogen at 35°C. “The residue was reconstituted in 100 μL of mobile phase at starting conditions.”…”

“…"The residue was reconstituted in 100 μL of mobile phase at starting conditions." 21 A comparison plasma solution was prepared in an appropriate manner to estimate the proven analytes quantitatively in the femoral blood samples by screening.…”