LC-MS/MS versus TLC plus GC methods: Consistency of glycerolipid and fatty acid profiles in microalgae and higher plant cells and effect of a nitrogen starvation

Jouhet, Juliette; Lupette, Josselin; Clerc, Olivier; Magneschi, Leonardo; Bedhomme, Mariette; Collin, Séverine; Roy, Sylvaine; Maréchal, Éric; Rébeillé, Fabrice

doi:10.1371/journal.pone.0182423

Cited by 90 publications

(82 citation statements)

References 47 publications

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“…To determine the lipid molecules that were present in each extract, 2D thinlayer chromatography with lipid extract from cells grown with or without P i was run as described previously (Abida et al, 2015). Each lipid spot was extracted from the silica, and each lipid molecule was identified by mass spectrometry according to Jouhet et al (2017).…”

Section: Lipid Content and Compositionmentioning

confidence: 99%

Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga

et al. 2017

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. Previous studies point to P limitation-induced changes in lipid composition. As, in microalgae, the molecular mechanisms of this specific P stress adaptation remain unresolved, we reveal a detailed phospholipid-recycling scheme in Nannochloropsis oceanica and describe important P acquisition genes based on highly corresponding transcriptome and lipidome data. Initial responses to P limitation showed increased expression of genes involved in P uptake and an expansion of the P substrate spectrum based on purple acid phosphatases. Increase in P trafficking displayed a rearrangement between compartments by supplying P to the chloroplast and carbon to the cytosol for lipid synthesis. We propose a novel phospholipid-recycling scheme for algae that leads to the rapid reduction of phospholipids and synthesis of the P-free lipid classes. P mobilization through membrane lipid degradation is mediated mainly by two glycerophosphoryldiester phosphodiesterases and three patatin-like phospholipases A on the transcriptome level. To compensate for low phospholipids in exponential growth, N. oceanica synthesized sulfoquinovosyldiacylglycerol and diacylglyceroltrimethylhomoserine. In this study, it was shown that an N. oceanica strain has a unique repertoire of genes that facilitate P acquisition and the degradation of phospholipids compared with other stramenopiles. The novel phospholipid-recycling scheme opens new avenues for metabolic engineering of lipid composition in algae.Phosphorus (P) availability is often a limiting factor in aquatic ecosystems (Van Mooy et al., 2009). In some microalgae, nutrient starvation limits cell division and leads to the accumulation of organic carbon (e.g. in the form of triacylglycerols [TAGs]). To cope with P limitation, the interplay of P recycling and changes in lipid classes is observed in the open sea and in culture studies of several stramenopiles and green algae (Van Mooy et al., 2009;Martin et al., 2011;Cañavate et al., 2017b). These studies showed a taxonomically diverse lipid response under P stress with unresolved questions related to the diversified mechanism behind the lipid responses. So far, only cursory insights into the lipidremodeling process have been provided by either transcriptomic or lipidomic studies on P limitation (Riekhof et al., 2005;Cruz de Carvalho et al., 2016;Shemi et al., 2016;Cañavate et al., 2017b). To capture the taxonomically diverse response and niche partitioning, studies include ecological successful algae, such as Emiliania huxleyi and Thalassiosira pseudonana (Lessard et al., 2005;Van Mooy et al., 2009), and algae featuring high resilience and lipid content during low P, like Phaeodactylum tricornutum and Nannochloropsis spp. (Rodolfi et al., 2009;Yang et al., 2013;Mayers et al., 2014;Abida et al., 2015). During these studies, focus is often on changes of long-chain fatty acids (FAs) and their contribution to lipid classes in specific pathways [OPEN] Articles can be viewed without a subscription. www.plantphysiol.org/cgi

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Section: Lipid Content and Compositionmentioning

confidence: 99%

Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga

et al. 2017

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“…Extraction and quantification were performed with three biological replicates. Glycerolipids were then analyzed and quantified by HPLC-tandem mass spectrometry, with appropriate standard lipids (Jouhet et al, 2017). In brief, lipid extracts corresponding to 25 nmol of total FAs were dissolved in 100 µL of chloroform:methanol (2:1, v/v) containing 125 pmol of each internal standard.…”

Section: Glycerolipid Extraction and Profilingmentioning

confidence: 99%

Non-Enzymatic Synthesis of Bioactive Isoprostanoids in the Diatom Phaeodactylum following Oxidative Stress

et al. 2018

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“…N and P are often limiting or co-limiting nutrients, and N is often more limiting than P in marine systems (Falkowski et al, 1998). In all microalgae that have been studied so far, either N or P deficiency was the main environmental factor that triggered TAG accumulation (Daboussi et al, 2014;Abida et al;, Jouhet et al, 2017. When N and/or P become limiting, cell proliferation decreases and TAGs start to accumulate as long as organic carbon remains available ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Ecophysiology and lipid dynamics of a eukaryotic mangrove decomposer

Dellero

Rose

Metton

et al. 2018

Environmental Microbiology

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Aurantiochytrium limacinum is an osmo-heterotrophic Stramenopile and a pioneering mangrove decomposer which is taxonomically assigned to the family of Thraustochytriaceae (class: Labyrinthulomycetes). The life cycle of A. limacinum involves different cell types including mono- and multi-nucleated cells as well as flagellated zoospores which colonize new fallen leaves. The ecological relevance of thraustochytrids is underestimated and eclipsed by their biotechnological importance, due to their ability to accumulate large amount of lipids, mainly triacylglycerols (TAGs). In this study, we aimed to understand the ecophysiological parameters that trigger zoospore production and the interplay between the life cycle of A. limacinum and its lipid metabolism. When grown in a rich medium, cells accumulated large amounts of TAGs at the end of their growth period, but no zoospores were produced. In poor media such as artificial sea water, zoospores were produced in massive quantities. In the absence of organic carbon, the zoospores remained swimming for at least 6 days, consuming their TAGs in the process. Addition of glucose rapidly triggered the maturation of the zoospores. On the basis of these data, we propose a life cycle for A. limacinum integrating the potential perturbations/changes in the environment surrounding a mangrove leaf that could lead to the production of zoospores and colonization of new areas.

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LC-MS/MS versus TLC plus GC methods: Consistency of glycerolipid and fatty acid profiles in microalgae and higher plant cells and effect of a nitrogen starvation

Cited by 90 publications

References 47 publications

Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga

Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga

Non-Enzymatic Synthesis of Bioactive Isoprostanoids in the Diatom Phaeodactylum following Oxidative Stress

Ecophysiology and lipid dynamics of a eukaryotic mangrove decomposer

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