LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B

Malik, Abdul; Aldakheel, Fahad M.; Syed, Rabbani; Alshehri, Meshal; Chaudhary, Anis Ahmad; Alkholief, Musaed; Alshamsan, Aws

doi:10.1016/j.jiph.2021.01.013

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…Experiments showed that this method had lower cost and higher detection efficiency compared with sequencing. However, the results obtained were reliable and consistent with the sequencing results [ 12 , 13 ]. The product of LCR can be detected by electrophoresis, autoradiography, and so on, but the process is complicated.…”

Section: Ligase-related Nucleic Acid Amplification Techniquessupporting

confidence: 88%

Advances in ligase-based nucleic acid amplification technology for detecting gene mutations: a review

Liu

Wang

et al. 2022

Mol Cell Biochem

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Gene mutation has been a concern for researchers because it results in genetic variations with base changes in molecular structure. Researchers continue to explore methods to detect gene mutations, which may help in disease diagnosis, medication guidance, and so on. Currently, the detection methods, such as whole-genome sequencing and polymerase chain reaction, have some limitations in terms of cost and sensitivity. Ligase (an enzyme) can recognize base mismatch as a commonly used tool in genetic engineering. Therefore, the ligase-related nucleic acid amplification technology for detecting gene mutations has become a research hotspot. In this study, the main techniques explored for detecting gene mutations included the ligase detection reaction, ligase chain reaction, rolling circle amplification reaction, enzyme-assisted polymerase chain reaction, and loop-mediated isothermal amplification reaction. This review aimed to analyze the aforementioned techniques and mainly present their advantages and disadvantages, sensitivity, specificity, cost, detection time, applications, and so on. The findings may help develop sufficient grounds for further studies on detecting gene mutations.

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Section: Ligase-related Nucleic Acid Amplification Techniquessupporting

confidence: 88%

Advances in ligase-based nucleic acid amplification technology for detecting gene mutations: a review

Liu

Wang

et al. 2022

Mol Cell Biochem

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“…Thus, LCR is a powerful method for the detection of single base mutation in single nucleotide polymorphism (SNP). Thermostable DNA ligase is a cornerstone for LCR due to its high fidelity and strong thermostability, thus allowing the useful DNA-based diagnostic tests for inherited diseases to be performed in clinical laboratories (Liu et al, 2020;Malik et al, 2021;Zhang et al, 2021;Li et al, 2022). The thermostable DNA ligases from T. thermophilus and T. aquaticus display the higher ligation fidelity than T4 DNA ligase (Barany, 1991;, and have been commercially used for LCR to date.…”

Section: Thermostable Dna Ligases: Ligase Chain Rection For Detection...mentioning

confidence: 99%

Thermostable DNA ligases from hyperthermophiles in biotechnology

et al. 2023

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DNA ligase is an important enzyme ubiquitous in all three kingdoms of life that can ligate DNA strands, thus playing essential roles in DNA replication, repair and recombination in vivo. In vitro, DNA ligase is also used in biotechnological applications requiring in DNA manipulation, including molecular cloning, mutation detection, DNA assembly, DNA sequencing, and other aspects. Thermophilic and thermostable enzymes from hyperthermophiles that thrive in the high-temperature (above 80°C) environments have provided an important pool of useful enzymes as biotechnological reagents. Similar to other organisms, each hyperthermophile harbors at least one DNA ligase. In this review, we summarize recent progress on structural and biochemical properties of thermostable DNA ligases from hyperthermophiles, focusing on similarities and differences between DNA ligases from hyperthermophilic bacteria and archaea, and between these thermostable DNA ligases and non-thermostable homologs. Additionally, altered thermostable DNA ligases are discussed. Possessing improved fidelity or thermostability compared to the wild-type enzymes, they could be potential DNA ligases for biotechnology in the future. Importantly, we also describe current applications of thermostable DNA ligases from hyperthermophiles in biotechnology.

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Highly specific screening of aspirin resistance-related single-nucleotide polymorphisms using ligase chain reaction strategy

Li,

Zhou,

Wang

et al. 2025

Talanta

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LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B

Cited by 3 publications

References 27 publications

Advances in ligase-based nucleic acid amplification technology for detecting gene mutations: a review

Advances in ligase-based nucleic acid amplification technology for detecting gene mutations: a review

Thermostable DNA ligases from hyperthermophiles in biotechnology

Highly specific screening of aspirin resistance-related single-nucleotide polymorphisms using ligase chain reaction strategy

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