LDH and LDH‐X activity in sperm from normospermic and oligozoospermic men

Abstract

Total lactate dehydrogenase (LDH) activity was measured in washed sperm from 74 oligozoospermic patients and 148 normospermic men. In the same samples, an additional iso-enzyme specific for germ cells, LDH-X was also measured. Both enzymatic activities were significantly higher in sperm from oligozoospermic patients than in those from normospermic men (P less than 0.001). In oligozoospermic patients, sperm LDH and LDH-X activities were related inversely to sperm concentration (r = -0.61 and r = -0.53, respectively). In normospermic men, this inverse relationship was less evident (r = -0.28 and r = -0.25, respectively). After separation of sperm on a discontinuous gradient of Percoll, LDH and LDH-X activities were increased in the non-migrated-sperm fractions and reduced in the migrated-sperm fractions in both groups of subjects when compared to the initial semen specimens. In addition, migrated sperm from oligozoospermic and normospermic men did not differ in their LDH and LDH-X activities. Although the possible contribution of immature germ-cells to these findings remains to be defined, the data suggest clearly that high levels of intracellular LDH and LDH-X are a biochemical feature of sperm from oligozoospermic subjects.

Evaluation of LDH Activity and Its Relationship with Fructose Levels in Seminal Plasma of Normospermic and Asthenospermic Males

“…of this enzyme from spermatozoa. In the study of Casano et al, LDH activity of oligospermic patients was higher than norospermic males, while it is inversely correlated with sperm concentration and percentage of motile sperms in both groups (34). In the present study, there was no correlation between the mentioned variables and LDH levels.…”

A purine nucleoside analogue-acyclovir [9-(2-hydroxyethoxymethyl)-9h-guanine] reversibly impairs testicular functions in mouse

“…The LDH estimation serves as a good indicator of cytotoxicity (Kumar et al, 2006) which functions as a chemical marker reflecting the degree of germ cell degeneration, and its presence in the testicular homogenate is related to the functional integrity of the seminiferous epithelium (Casano et al, 1991). The LDH level was increased significantly in the testis indicating extensive tissue damage (Kumar et al, 2006).…”

Effects of Hyaluronic Acid on Ca²⁺ Influx, Lactate Dehydrogenase Activity, and Cyclic AMP Synthesis in Canine Ejaculated Sperm during <i>In Vitro</i> Capacitation

“…GAGs, including hyaluronic acid [44] and heparin [14,41], accelerate Ca 2+ influx into sperm cytoplasm. LDH enzyme activity has been found in the sperm of many species [6,7,17,24,35,42] and is required for energy metabolism in their sperm [17,35]. High LDH activity stimulates sperm capacitation [35].…”

“…The cumulus intercellular matrix of oocytes is comprised of hyaluronic acid in many species [3,33,43] It is well known that sperm capacitation is induced by Ca 2+ influx into the cytoplasm of sperm [11,41]. Energy metabolism of sperm is closely related to the activity of lactate dehydrogenase (LDH) enzyme in the sperm [6,7,17,24,35,41], and high LDH activity is maintained during sperm capacitation [35]. The flagellar movement of the canine sperm tail has been reported to be induced by an increase in cyclic adenosine monophosphate (cAMP) concentration in the sperm [47].…”

“…Energy metabolism of sperm is closely related to the activity of lactate dehydrogenase (LDH) enzyme in the sperm [6,7,17,24,35,41], and high LDH activity is maintained during sperm capacitation [35]. The flagellar movement of the canine sperm tail has been reported to be induced by an increase in cyclic adenosine monophosphate (cAMP) concentration in the sperm [47].…”