Le.MAPK and its interacting partner, Le.DRMIP, in fruiting body development in Lentinula edodes

Szeto, Carol Y.Y.; Leung, Grace; Kwan, Hoi Shan

doi:10.1016/j.gene.2007.01.030

Cited by 36 publications

(30 citation statements)

References 28 publications

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“…The putative Fpp1 (Fig. 2) also displays a modular structure encompassing (i) an N-terminal signal peptide of 25 aa; (ii) a region similar to other metalloproteases (MEROPS M12, adamalysin family), with a typical HEXXHXXGXXH zinc metalloprotease motif (amino acids 364-374); (iii) a Drmip-Hesp domain, characteristic of a mitogen-activated protein kinase involved in growth and development in many organisms (Szeto et al, 2007) in the central region (amino acids 600-675), and a regulatory P domain (amino Fig. 1.…”

Section: Resultsmentioning

confidence: 99%

Comparative analysis and mutation effects of fpp2–fpp1 tandem genes encoding proteolytic extracellular enzymes of Flavobacterium psychrophilum

et al. 2011

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Flavobacterium psychrophilum is a very significant fish pathogen that secretes two biochemically characterized extracellular proteolytic enzymes, Fpp1 and Fpp2. The genes encoding these enzymes are organized as an fpp2–fpp1 tandem in the genome of strain F. psychrophilum THC02/90. Analysis of the corresponding encoded proteins showed that they belong to two different protease families. For gene function analysis, new genetic tools were developed in F. psychrophilum by constructing stable isogenic fpp1 and fpp2 mutants via single-crossover homologous recombination. RT-PCR analysis of wild-type and mutant strains suggested that both genes are transcribed as a single mRNA from the promoter located upstream of the fpp2 gene. Phenotypic characterization of the fpp2 mutant showed lack of caseinolytic activity and higher colony spreading compared with the wild-type strain. Both characteristics were recovered in the complemented strain. One objective of this work was to assess the contribution to virulence of these proteolytic enzymes. LD50 experiments using the wild-type strain and mutants showed no significant differences in virulence in a rainbow trout challenge model, suggesting instead a possible nutritional role. The gene disruption procedure developed in this work, together with the knowledge of the complete genome sequence of F. psychrophilum, open new perspectives for the study of gene function in this bacterium.

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Section: Resultsmentioning

confidence: 99%

Comparative analysis and mutation effects of fpp2–fpp1 tandem genes encoding proteolytic extracellular enzymes of Flavobacterium psychrophilum

et al. 2011

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“…The functions of these proteins in the Ascomycota are unclear, but in some Basidiomycota, they appear to be involved in both fruiting body formation and infection cell development (Link and Voegele 2008). One member of this family from the Shitake mushroom interacts with a fruiting body formation-induced MAP kinase in the yeast two-hybrid system (Szeto et al 2007). Conceivably these proteins could interact to link information about cell wall surface architecture to calcium or MAP kinase signaling pathways.…”

Section: Resultsmentioning

confidence: 99%

Temporal and Spatial Regulation of Gene Expression During Asexual Development ofNeurospora crassa

et al. 2010

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In this study we profiled spatial and temporal transcriptional changes during asexual sporulation in the filamentous fungus Neurospora crassa. Aerial tissue was separated from the mycelium to allow detection of genes specific to each tissue. We identified 2641 genes that were differentially expressed during development, which represents 25% of the predicted genes in the genome of this model fungus. On the basis of the distribution of functional annotations of 1102 of these genes, we identified gene expression patterns that define key physiological events during conidial development. Not surprisingly, genes encoding transcription factors, cell wall remodeling proteins, and proteins involved in signal transduction were differentially regulated during asexual development. Among the genes differentially expressed in aerial tissues the majority were unclassified and tended to be unique to ascomycete genomes. This finding is consistent with the view that these genes evolved for asexual development in the Pezizomycotina. Strains containing deletions of several differentially expressed genes encoding transcription factors exhibited asexual development-associated phenotypes. Gene expression patterns during asexual development suggested that cAMP signaling plays a critical role in the transition from aerial growth to proconidial chain formation. This observation prompted us to characterize a deletion of the gene encoding a highaffinity cAMP phosphodiesterase (NCU00478). NCU00478 was determined to be allelic to aconidiate-2, a previously identified genetic locus controlling conidiation.

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“…In the Basidiomycete fungus Lentinula edodes (shiitake mushroom), the PF10342 domain-containing protein Le.DRMIP, which also possesses a mitochondrial targeting signal peptide and transmembrane domain, interacts with the developmentally regulated mitogen-activated protein kinase Le.MAPK. Both proteins have been proposed to play a role in cell differentiation during fruiting body development (Szeto et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Specific Hypersensitive Response–Associated Recognition of New Apoplastic Effectors from Cladosporium fulvum in Wild Tomato

Mesarich

Ökmen

Rövenich

et al. 2018

MPMI

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Tomato leaf mold disease is caused by the biotrophic fungus Cladosporium fulvum. During infection, C. fulvum produces extracellular small secreted protein (SSP) effectors that function to promote colonization of the leaf apoplast. Resistance to the disease is governed by Cf immune receptor genes that encode receptor-like proteins (RLPs). These RLPs recognize specific SSP effectors to initiate a hypersensitive response (HR) that renders the pathogen avirulent. C. fulvum strains capable of overcoming one or more of all cloned Cf genes have now emerged. To combat these strains, new Cf genes are required. An effectoromics approach was employed to identify wild tomato accessions carrying new Cf genes. Proteomics and transcriptome sequencing were first used to identify 70 apoplastic in planta-induced C. fulvum SSPs. Based on sequence homology, 61 of these SSPs were novel or lacked known functional domains. Seven, however, had predicted structural homology to antimicrobial proteins, suggesting a possible role in mediating antagonistic microbe-microbe interactions in planta. Wild tomato accessions were then screened for HR-associated recognition of 41 SSPs, using the Potato virus X-based transient expression system. Nine SSPs were recognized by one or more accessions, suggesting that these plants carry new Cf genes available for incorporation into cultivated tomato.Leaf mold disease of tomato (Solanum lycopersicum) is caused by the biotrophic Dothideomycete fungal pathogen Cladosporium fulvum (syn. Passalora fulva and Fulvia fulva) (Thomma et al. 2005). The fungus likely originated in South America, the center of origin for tomato (Jenkins 1948), with Nucleotide sequence data is available in the GenBank database under the following accession numbers: Ecp6, KX943112; Ecp7, KX943113; Ecp8, KX943038; Ecp9-1, KX943041; Ecp9-2, KX943114; Ecp9-3, KX943115; Ecp9-4, KX943116; Ecp9-5, KX943117; Ecp9-6, KX943118; Ecp9-7, KX943119; Ecp9-8, KX943120; Ecp9-9, KX943081; Ecp10-1, KX943046; Ecp10-2, KX943063; Ecp10-3, KX943121; Ecp11-1, KX943050; Ecp12, KX943058; Ecp13, KX943065; Ecp14-1, KX943087; Ecp14-2, KX943122; Ecp15, KX943091; Ecp16, KX943080; Ecp17, KX943051; Ecp18, KX943035; Ecp19-1, KX943036; Ecp19-2, KX943048; Ecp20-1, KX943037; Ecp20-2, KX943057; Ecp20-3, KX943096; Ecp21-1, KX943039; Ecp22, KX943040; Ecp23, KX943044; Ecp24-1, KX943045; Ecp24-2, KX943094; Ecp25, KX943047; Ecp26, KX943052; Ecp27, KX943054; Ecp28-1, KX943056; Ecp28-2, KX943088; Ecp28-3, KX943090; Ecp29, KX943103; Ecp30, KX943076; Ecp31, KX943059; Ecp32-1, KX943062; Ecp32-2, KX943101; Ecp33, KX943066; Ecp34, KX943067; Ecp35, KX943068; Ecp36-1, KX943069; Ecp37, KX943072; Ecp38, KX943073; Ecp39, KX943074; Ecp40, KX943079; Ecp41, KX943082; Ecp42, KX943083; Ecp43-1, KX943089; Ecp44, KX943092; Ecp45, KX943093; Ecp46, KX943095; Ecp47, KX943097; Ecp48, KX943098; Ecp49-1, KX943099; Ecp50-1, KX943100; Ecp51, KX943102; Ecp52, KX943104; Ecp53-1, KX943105; Ecp54-1, KX943107; Ecp55, KX943108; Ecp56, KX943110; Ecp57-1,

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Le.MAPK and its interacting partner, Le.DRMIP, in fruiting body development in Lentinula edodes

Cited by 36 publications

References 28 publications

Comparative analysis and mutation effects of fpp2–fpp1 tandem genes encoding proteolytic extracellular enzymes of Flavobacterium psychrophilum

Comparative analysis and mutation effects of fpp2–fpp1 tandem genes encoding proteolytic extracellular enzymes of Flavobacterium psychrophilum

Temporal and Spatial Regulation of Gene Expression During Asexual Development ofNeurospora crassa

Specific Hypersensitive Response–Associated Recognition of New Apoplastic Effectors from Cladosporium fulvum in Wild Tomato

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