Leaching and in vitro bio accessibility of aluminium from different teas

Abstract

and the risk of Al, it is important to evaluate total Al content in tea, the leaching of Al from the tea, and the bio-accessibility of Al from its infusion after digestion at the same time. It is also necessary to compare the difference of total Al contents in green tea, black tea, and oolong tea, of the Al leaching from these teas, and of the bio-accessibility of Al from their infusions because green tea, black tea, and oolong tea were processed with different manufacturing processed, and different manufacturing processed might lead to the different effects on the total Al content in tea, thus affecting the leaching of Al from them and the bio-accessibility of Al from their infusion. The present study aims to determinate total Al contents in green tea, black tea, and oolong tea processed with same fresh leaves and the leaching of Al from these teas, and to evaluate the bio-accessibility of Al from their infusions under simulated gastrointestinal conditions. Materials and methods MaterialsPorcine pepsin was purchased from Sigma-Aldrich Chemical Company (St. Louis, MO). Porcine pancreatin (CAS. 8049-47-6) and porcine stomach mucin were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (China). Porcine pancreatic lipase (CAS. 9001-62-1) was purchased from Shanghai Aladdin Industrial Inc. (China). All reagents and chemicals used were of analytical grade. Processing of teaFresh tea leaves of Camellia sinensis L. were harvested from tea

Simplified Method of Multi-Elemental Analysis of Dialyzable Fraction of Tea Infusions by FAAS and ICP OES

“…Despite high concentrations of Al in infusions of analyzed teas, it was found that only a small part of this metal was available for absorption in the gastrointestinal tract. These results were in good agreement with results of studies undertaken by other authors for infusions of different BTs and GTs [22,23,32,33].…”

“…Resulted sample solutions were diluted (1:1), acidified with HNO 3 to 0.11 mol L −1 , and measured by ICP OES. Lin and Yang [23] determined bioavailability of Al from infusions of three teas (GT, BT, and oolong tea), but without membrane ultrafiltration. Samples of infusions after in vitro GID were centrifuged, and their supernatants were analyzed by FAAS.…”