Lead Acetate-Induced Hepatoxicity in Wistar Rats: Possible Protective Role of Combination Therapy

Sharma, Samta; Raghuvanshi, Suchita; Jaswal, Amita; Shrivastava, Sadhana; Shukla, Sangeeta

doi:10.1615/jenvironpatholtoxicoloncol.2015012006

Cited by 10 publications

(8 citation statements)

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“…Moreover, the liver is the body’s largest detoxification organ, a lot of detoxification enzymes, such as cysteine proteases, will be affected by lead. Previous studies have shown that lead exposure can cause oxidative damage in the liver by inhibiting the activities of antioxidant enzymes, decreasing the concentration of non-enzymatic antioxidants and increasing the concentration of reactive oxygen species [36,37,38]. Our results showed that lead acetate added to the drinking water for six weeks for mice resulted in severe histopathological changes in the liver tissue.…”

Section: Discussionsupporting

confidence: 47%

Proanthocyanidins Attenuation of Chronic Lead-Induced Liver Oxidative Damage in Kunming Mice via the Nrf2/ARE Pathway

et al. 2016

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Lead is harmful for human health and animals. Proanthocyanidins (PCs), a natural antioxidant, possess a broad spectrum of pharmacological and medicinal properties. However, its protective effects against lead-induced liver damage have not been clarified. This study was aimed to evaluate the protective effect of PCs on the hepatotoxicity of male Kunming mice induced by chronic lead exposure. A total of 70 healthy male Kunming mice were averagely divided into four groups: control group, i.e., the group exposed to lead, the group treated with PCs, and the group co-treated with lead and PCs. The mice exposed to lead were given water containing 0.2% lead acetate. Mice treated in the PCs and PCs lead co-treated groups were given PC (100 mg/kg) in 0.9% saline by oral gavage. Lead exposure caused a significant elevation in the liver function parameters, lead level, lipid peroxidation, and inhibition of antioxidant enzyme activities. The induction of oxidative stress and histological alterations in the liver were minimized by co-treatment with PCs. Meanwhile, the number of Transferase-Mediated Deoxyuridine Triphosphate-Biotin Nick End Labeling (TUNEL)-positive cells was significantly reduced in the PCs/lead co-treated group compared to the lead group. In addition, the lead group showed an increase in the expression level of Bax, while the expression of Bcl-2 was decreased. Furthermore, the lead group showed an increase in the expression level of endoplasmic reticulum (ER) stress-related genes and protein (GRP78 and CHOP). Co-treated with PCs significantly reversed these expressions in the liver. PCs were, therefore, demonstrated to have protective, antioxidant, and anti-ER stress and anti-apoptotic activities in liver damage caused by chronic lead exposure in the Kunming mouse. This may be due to the ability of PCs to enhance the ability of liver tissue to protect against oxidative stress via the Nrf2/ARE signaling pathway, resulting in decreasing ER stress and apoptosis of liver tissue.

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Section: Discussionsupporting

confidence: 47%

Proanthocyanidins Attenuation of Chronic Lead-Induced Liver Oxidative Damage in Kunming Mice via the Nrf2/ARE Pathway

et al. 2016

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“…In our study, we evaluated the effects of the ozone and selenium applications on alcoholic liver damage using histopathological analyses and assessment of serum AST and ALT levels. It has been reported in various studies that AST and ALT levels increase independently of the cause of the damage (19)(20)(21). It has also been reported that selenium application with N-acetylcysteine has a protective effect on lead and mercury toxicity (19,20), lowers ALT activity in thioacetamide-induced cirrhosis and AST and ALT in arsenic toxicity (21).…”

Section: Discussionmentioning

confidence: 98%

“…It has been reported in various studies that AST and ALT levels increase independently of the cause of the damage (19)(20)(21). It has also been reported that selenium application with N-acetylcysteine has a protective effect on lead and mercury toxicity (19,20), lowers ALT activity in thioacetamide-induced cirrhosis and AST and ALT in arsenic toxicity (21). In the case of alcohol, it is reported that ebselen, an organoselenium compound, reduces elevated ALT in rats treated with alcohol (22).…”

Section: Discussionmentioning

confidence: 99%

“…In a study investigating the effects of selenium enriched probiotics on liver fibrosis induced by carbon tetrachloride, selenium was found to suppress hepatic inflammation and reduce hepatic stellate cells by inducing apoptosis (27). In addition, N-acetylcysteine (NAC) and selenium, applied as combination therapy, have been shown to have a marked positive effect on histopathological findings in animals exposed to lead (19). There are no studies investigating the effects of ozone on apoptotic hepatocytes, inflammation, necrosis and steatosis (32,33), which is reported in histopathological analyses in alcoholic liver disease.…”

Section: (A) Liver Tissue With Vascular Congestion In the Control Gmentioning

confidence: 99%

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Efficacy of Ozone and Selenium Therapy for Alcoholic Liver Injury: An Experimental Model

Adalı

Eroğlu

Makav

et al. 2019

In Vivo

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Background/Aim: Alcoholic liver disease is an important health problem which is reversible during early stages of liver damage, but becomes permanent with time. Nowadays, many studies focus on various agents that prevent, reduce or slow the progression of the toxic effects of alcohol. In our study, we investigated the efficiency of ozone and selenium against oxidative damage in a model of alcohol-induced liver damage. Materials and Methods: Forty-eight female Wistar Albino rats between 4 and 6 months of age and weighing 190-250 g were included in the study and were used as models of alcohol liver damage. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum and tissue total oxidant levels, serum and tissue total antioxidant levels, and the histopathological evaluation of the liver were performed in 8 groups. Results: In the statistical analysis, it was observed that ozone and/or selenium therapies decreased the AST levels. Total oxidant and antioxidant serum levels were found to vary in serum and tissue. Ozone and/or selenium therapies decreased liver damage, according to histopathological findings. Conclusion: Through ozone and/or selenium therapies, less damage was observed histopathologically compared to the alcohol group. It is thought that the results of our study can be used in individual treatments following confirmation of liver damage in alcoholic patients.

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“…However, a neuroscientist in the Rural Environments Group noted that lead poisoning is, in fact, associated with changes in thyroid function, although a specific connection between lead toxicity and thyroid cancer has not been conclusively demonstrated. Lead poisoning is associated with a decrease in circulating levels of thyroid hormone distributor proteins in lab animals [18,19] and in the cerebral spinal fluid of humans [20], suggesting this as a possible mechanism by which lead poisoning may lead to an overgrowth of the thyroid gland. This insight contributed to the Rural Environments Group's determination that further investigations into the lead emissions from open burning at RAAP were warranted.…”

Section: University and Faculty Support Of Transdisciplinary Communitmentioning

confidence: 99%

Building Interdisciplinary Partnerships for Community-Engaged Environmental Health Research in Appalachian Virginia

Satterwhite

Bell

Marr

et al. 2020

IJERPH

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This article describes a collaboration among a group of university faculty, undergraduate students, local governments, local residents, and U.S. Army staff to address long-standing concerns about the environmental health effects of an Army ammunition plant. The authors describe community-responsive scientific pilot studies that examined potential environmental contamination and a related undergraduate research course that documented residents’ concerns, contextualized those concerns, and developed recommendations. We make a case for the value of resource-intensive university–community partnerships that promote the production of knowledge through collaborations across disciplinary paradigms (natural/physical sciences, social sciences, health sciences, and humanities) in response to questions raised by local residents. Our experience also suggests that enacting this type of research through a university class may help promote researchers’ adoption of “epistemological pluralism”, and thereby facilitate the movement of a study from being “multidisciplinary” to “transdisciplinary”.

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Lead Acetate-Induced Hepatoxicity in Wistar Rats: Possible Protective Role of Combination Therapy

Cited by 10 publications

References 0 publications

Proanthocyanidins Attenuation of Chronic Lead-Induced Liver Oxidative Damage in Kunming Mice via the Nrf2/ARE Pathway

Proanthocyanidins Attenuation of Chronic Lead-Induced Liver Oxidative Damage in Kunming Mice via the Nrf2/ARE Pathway

Efficacy of Ozone and Selenium Therapy for Alcoholic Liver Injury: An Experimental Model

Building Interdisciplinary Partnerships for Community-Engaged Environmental Health Research in Appalachian Virginia

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