Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro

Abstract

Background and purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation. Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO 3) 2 concentrations were used: 0, 10-6 , 10-5 , 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content. Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO 3) 2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO 3) 2 media. Thus, the concentration of 10-4 M Pb(NO 3) 2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype "Villafranca" (almost 200% and 125% higher, respectively).

In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium

“…In concordance with the results from some previous works (Kališova-Špirochova et al 2003, Katanić et al 2007, di Lonardo et al 2011, Kovačević et al 2013a, in most of the examined media no signs of intoxication were found on shoots. In this work, only sporadic necrotic changes were found on the roots formed in medium with 10 -3 M Pb(NO 3 ) 2 , which is consistent with the results by Kovačević et al (2013a) on the same medium. However, Bojarczuk (2004) reported an inhibitory effect of the medium with pH 5.5 and 2.0 mM Pb(NO 3 ) 2 on shoot and root development of calli of hybrid aspen on regeneration medium.…”

“…Five white poplar genotypes were examined: Villafranca (Italy), L-12 (Serbia), L-80 (Serbia), LBM (Serbia), and LCM (Serbia). These genotypes have been proved suitable for biomass production, landscaping, and horticulture and suitable for in vitro testing to abiotic stresses (Kovačević et al 2013a, Vuksanović et al 2016, 2019a. According to Kovačević and Igić (2018) and Kovačević et al (2020), Villafranca, L-12 and L-80 genotypes are characterized by relatively good rooting of hardwood cuttings.…”

“…Micropropagation of five tested genotypes was performed by shoot tips and axillary buds to preserve clonal fidelity Raina 2000, Confalonieri et al 2003). The medium used in shoot multiplication has been described by Kovačević et al (2013a).…”

Fiber Characteristics of White Poplar (Populus alba L.) Juvenile Wood along the Drava River

“…For a long period of time, white poplar has not been considered as a commercially important wood species. Due to climate changes and changed ecological conditions, it is becoming more interesting for breeding as a drought tolerant species (Eggens et al, 1972;Gilman and Watson, 1994;Isebrands and Richardson, 2014).…”

Pathological Brain Detection by Artificial Intelligence in Magnetic Resonance Imaging Scanning (Invited Review)

“…It can solve both stochastic optimization problems and deterministic optimization problems. The injected randomness can guarantee the search less sensitive to modeling errors and escape from local minima points [261]. The common SO methods include simulated annealing (SA) [262], quantum annealing (QA) [263], random search, etc.…”