Leaf architecture and petiole anatomy of Philippine Dipterocarpus species (Dipterocarpaceae)

Abstract

The study investigated the leaf architecture and petiole anatomy of eight Dipterocarpus (Dipterocarpaceae) species growing in Mount Makiling Forest Reserve (MMFR) in the Philippines to delineate the species, especially during their non-flowering phase. Leaf and petiole samples from MMFR were examined following the manual on leaf architecture studies. Freehand technique was used to examine the stomata and petiole characters. A UPGMA phenogram was then generated to determine the relationships among Dipterocarpus species using 26 leaf and petiole characters. The most useful characters to delineate Dipterocarpus species are the presence of trichomes, the arrangement of vascular bundles (medullary and outer vascular bundles), areolation, fimbrial vein, and leaf size. Other characters viz., the laminar ratio, blade class, laminar shape, base shape, margin type, and intersecondary, and layers and shape of parenchyma and collenchyma cells were useful as unifying characters. Therefore, certain characters of the leaf architecture and petiole anatomy may be used as taxonomic markers to delineate and correlate the Dipterocarpus species in the Philippines particularly during the nonflowering phase of the species. However, further investigations using samples from the wild populations of the species and molecular techniques may be done to elucidate the taxonomic use of the characters presented in this study.
 Bangladesh J. Plant Taxon. 27(1): 1-14, 2020 (June)

Short communication: Leaf architecture of 35 species of Dipterocarpaceae cultivated in Forest Area with Special Purposes in Carita, Banten, Indonesia

“…In addition, the determination key was formulated based on leaf architecture character. This analysis commonly used in clustering especially using morphological data (Hernandez et al 2020).…”

“…The petiole anatomy has proven to be useful as an identification tool in Shorea spp. (Hernandez et al 2020). Several Shorea species and Dipterocarpus gracilis have slightly geniculate petiole.…”

“…Leaf architecture is a breakthrough to confirm a species based on venation pattern . A leaf's venation pattern is a significant character in the taxonomy's determination (Inamdar and Murthy 1978;Celadiña et al 2012;Pulan and Buot Jr. 2014;Hernandez et al 2020). Therefore, investigations are required to understand the leaf architecture of Dipterocarpaceae species, for taxonomy and forestry.…”

Short Communication: Leaf architectural analysis of confusing Syzygium species: Syzygium aqueum (Burm.f.) Alston and Syzygium samarangense (Blume) Merr. & L.M.Perry (Myrtaceae)

“…Reports have showed that these distinctive characters can be used as taxonomic characters at species level. For instance, distinct areolation among species have been reported to be one of the most useful leaf characters used as taxonomic tool for delimitation, as seen in the study of Khaya species by Oyedapo et al (2018) and Philippine Dipterocarpus species by Hernandez et al (2020). Moreover, taxonomic significance of leaf apex shape and size have also been elucidated by studies of Shorea species by Khan et al (2016) and Pulan and Buot (2014).…”

Effects of Irrigation and Fertilization on the Morpho-Physiological Traits of <em>Populus sibirica</em> Hort. Ex Tausch and <em>Ulmus pumila</em> L. In the Semi-arid Steppe Region of Mongolia

“…The leaves were photo-scanned using an HP LaserJet scanner (M1132 MFP, Palo Alto, CA, USA) with a 600 dpi resolution. ImageJ processing software was used to analyze the LA of the samples collected following the procedures in Schneider et al [51] and the modified procedure in Hernandez et al [52].…”