Leaf-based induction of protocorm-like bodies, their encapsulation, storage and post-storage germination with genetic fidelity in Mokara Sayan × Ascocenda Wangsa gold

Gantait, Saikat; Subrahmanyeswari, Tsama; Sinniah, Uma Rani

doi:10.1016/j.sajb.2022.08.012

Cited by 5 publications

(1 citation statement)

References 35 publications

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“…The protocorms were best encapsulated at 3% sodium alginate and 75 mM calcium chloride. Furthermore, 71.2% germination frequency displayed by synthetic seeds stored at 25 • C even after 180 days, while those stored at 4 • C degenerated completely [123]. Orchis militaris L. Seeds mM (Malmgren modified terrestrial orchid medium) + CW (5%) + birch sap (5%) + AC (0.1%) produced the highest seed germination (82.6%).…”

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confidence: 96%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

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confidence: 96%