Leaf morphology and chlorophyll fluorescence characteristics of mulberry seedlings under waterlogging stress

Rao, Liangyi; Liu, Siyuan; Cui, Xue

doi:10.1038/s41598-021-92782-z

Cited by 44 publications

(33 citation statements)

References 14 publications

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“…Most of the chlorophyll fluorescence parameters measured in light-and dark-adapted cucumber leaves were significantly reduced under 10-day waterlogging stress, indicating the sensitivity of cucumber plants to hypoxia stress. Previous studies demonstrated waterlogging causes a significant decline in chlorophyll fluorescence parameters, including F o , and F m , which reflect the PSII electron transport capacity in dark-adapted leaves (Smethurst and Shabala, 2003;Rao et al, 2021;Olorunwa et al, 2022). However, in the current study, although F o decreased, F m did not change significantly after 10 DOW in cucumber plants.…”

Section: Discussioncontrasting

confidence: 81%

Short waterlogging events differently affect morphology and photosynthesis of two cucumber (Cucumis sativus L.) cultivars

et al. 2022

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Waterlogging induces growth and developmental changes in sensitive crops such as cucumber (Cucumis sativus L.) during early plant development. However, information on the physiological mechanisms underpinning the response of cucumber plants to waterlogging conditions is limited. Here, we investigated the effects of 10-day waterlogging stress on the morphology, photosynthesis, and chlorophyll fluorescence parameters in two cultivars of cucumber seedlings. Waterlogging stress hampered cultivars’ growth, biomass accumulation, and photosynthetic capacity. Both cultivars also developed adventitious roots (ARs) after 10 days of waterlogging (DOW). We observed differential responses in the light- and carbon-dependent reactions of photosynthesis, with an increase in light-dependent reactions. At the same time, carbon assimilation was considerably inhibited by waterlogging. Specifically, the CO2 assimilation rate (A) in leaves was significantly reduced and was caused by a corresponding decrease in stomatal conductance (gs). The downregulation of the maximum rate of Rubisco efficiency (Vcmax) and the maximum rate of photosynthetic electron transport (Jmax) were non-stomatal limiting factors contributing to A reduction. Exposure of cucumber to 10 DOW affected the PSII photochemistry by downregulating the PSII quantum yield (ΦPSII). The redox state of the primary quinone acceptor in the lake model (1-qL), a measure of the regulatory balance of the light reactions, became more oxidized after 10 DOW, indicating enhanced electron sink capacity despite a reduced A. Overall, the results suggest that waterlogging induces alterations in the photochemical apparatus efficiency of cucumber. Thus, developing cultivars that resist inhibition of PSII photochemistry while maintaining carbon metabolism is a potential approach for increasing crops’ tolerance to waterlogged environments.

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Section: Discussioncontrasting

confidence: 81%

Short waterlogging events differently affect morphology and photosynthesis of two cucumber (Cucumis sativus L.) cultivars

et al. 2022

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“…Most of the chlorophyll fluorescence parameters measured in light-and dark-adapted cucumber leaves were significantly reduced under 10-day waterlogging stress, indicating the sensitivity of cucumber plants to hypoxia stress. Previous studies demonstrated waterlogging causes a significant decline in chlorophyll fluorescence parameters, including F o , and F m, which reflect the PSII electron transport capacity in dark-adapted leaves (Smethurst & Shabala 2003;Rao, Li & Cui 2021;Olorunwa et al 2022). However, in the current study, although F o decreased, F m did not change significantly after 10 DOW in cucumber plants.…”

Section: Discussionmentioning

confidence: 95%

Waterlogging induces alterations in cucumber ( Cucumis sativus L.) photosynthesis, chlorophyll fluorescence, and responses to CO 2 and light intensity.

Olorunwa

Adhikari

Brazel

et al. 2022

Preprint

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Waterlogging induces growth and developmental changes in sensitive crops such as cucumber ( Cucumis sativus L.) during early plant development. However, information on the physiological mechanisms underpinning the response of cucumber plants to waterlogging conditions is limited. Here, we investigated the effects of 10-day waterlogging stress on the morphology, photosynthesis, and chlorophyll fluorescence parameters in two cultivars of cucumber seedlings. Waterlogging stress hampered cultivars’ growth, biomass accumulation, and photosynthetic capacity. Both cultivars also developed adventitious roots (ARs) after 10 days of waterlogging (DOW). We observed differential responses in the light- and carbon-dependent reactions of photosynthesis, with an increase in light-dependent reactions. At the same time, carbon assimilation was considerably inhibited by waterlogging. Specifically, the CO assimilation rate ( A) in leaves was significantly reduced and was caused by a corresponding decrease in stomatal conductance (g ). The downregulation of the maximum rate of Rubisco (V ) and the maximum rate of photosynthetic electron transport (J ) were non-stomatal limiting factors contributing to A reduction. Exposure of cucumber to 10 DOW affected the PSII photochemistry by downregulating the PSII quantum yield (Φ ). The redox state of the primary quinone acceptor in the lake model (1-qL), a measure of the regulatory balance of the light reactions, became more oxidized after 10 DOW, indicating enhanced electron sink capacity despite a reduced A. Overall, the results suggest that waterlogging induces alterations in the photochemical apparatus efficiency of cucumber. Thus, developing cultivars that resist photodamage while maintaining PSII photochemistry is a potential approach for increasing crops’ tolerance to waterlogged environments.

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“… 21 22 After 90 days of soaking, the germination rate of mulberry seedlings could reach 62.6%. 23 Rao et al 24 found that during flooding, the leaves of seedlings treated with shallow submergence remained healthy, while the leaves treated with half submergence and full submergence showed different degrees of waterlogging symptoms in the middle and late flooding periods, and formed adventitious roots at the stem base.…”

Section: Introductionmentioning

confidence: 99%

“…39 However, the fluorescence parameters of mulberry showed a relatively stable trend in the late flooding period. 24 …”

Section: Introductionmentioning

confidence: 99%

Physiological and comparative transcriptome analysis of the response and adaptation mechanism of the photosynthetic function of mulberry (Morus alba L.) leaves to flooding stress

Quan

Sun

Liu

et al. 2022

Plant Signaling & Behavior

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Flooding has become one of the major abiotic stresses that seriously affects plant growth and development owing to changes in the global precipitation pattern. Mulberry ( Morus alba L.) is a desirable tree spePhysocarpus amurensis Maxim andcies with high ecological and economic benefits. To reveal the response and adaptive mechanisms of the photosynthetic functions of mulberry leaves to flooding stress, chlorophyll synthesis, photosynthetic electron transfer and the Calvin cycle were investigated by physiological studies combined with an analysis of the transcriptome. Flooding stress inhibited the synthesis of chlorophyll (Chl) and decreased its content in mulberry leaves. The sensitivity of Chl a to flooding stress was higher than that of Chl b owing to the changes of CHLG (LOC21385082) and CAO (LOC21408165) that encode genes during chlorophyll synthesis. The levels of expression of Chl b reductase NYC (LOC112094996) and NYC (LOC21385774), which are involved in Chl b degradation, were upregulated on the fifteenth day of flooding, which accelerated the transformation of Chl b to Chl a , and upregulated the expression of PPH (LOC21385040) and PAO (LOC21395013). This accelerated the degradation of chlorophyll. Flooding stress significantly inhibited the photosynthetic function of mulberry leaves. A Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes under different days of flooding stress indicated significant enrichment in Photosynthesis-antenna proteins (map00196), Photosynthesis (map00195) and Carbon fixation in photosynthetic organisms (map00710). On the fifth day of flooding, 7 and 5 genes that encode antenna proteins were identified on LHCII and LHCI, respectively. They were significantly downregulated, and the degree of downregulation increased as the trees were flooded longer. Therefore, the power of the leaves to capture solar energy and transfer this energy to the reaction center was reduced. The chlorophyll fluorescence parameters and related changes in the expression of genes in the transcriptome indicated that the PSII and PSI of mulberry leaves were damaged, and their activities decreased under flooding stress. On the fifth day of flooding, electron transfer on the PSII acceptor side of mulberry leaves was blocked, and the oxygen-evolving complex (OEC) on the donor side was damaged. On the tenth day of flooding, the thylakoid membranes of mulberry leaves were damaged. Five of the six coding genes that mapped to the OEC were significantly downregulated. Simultaneously, other coding genes located at the PSII reaction center and those located at the PSI reaction center, including Cytb6/f, PC, Fd, FNR and ATP, were also significantly down...

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Leaf morphology and chlorophyll fluorescence characteristics of mulberry seedlings under waterlogging stress

Cited by 44 publications

References 14 publications

Short waterlogging events differently affect morphology and photosynthesis of two cucumber (Cucumis sativus L.) cultivars

Short waterlogging events differently affect morphology and photosynthesis of two cucumber (Cucumis sativus L.) cultivars

Waterlogging induces alterations in cucumber ( Cucumis sativus L.) photosynthesis, chlorophyll fluorescence, and responses to CO 2 and light intensity.

Physiological and comparative transcriptome analysis of the response and adaptation mechanism of the photosynthetic function of mulberry (Morus alba L.) leaves to flooding stress

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