Learning and memory retention deficits in prepubertal guinea pigs prenatally exposed to low levels of the organophosphorus insecticide malathion

Lumsden, Eric W.; McCowan, Lillian; Pescrille, Joseph D.; Fawcett, William P.; Chen, Hegang; Albuquerque, Edson X.; Mamczarz, Jacek; Pereira, Edna F. R.

doi:10.1016/j.ntt.2020.106914

Cited by 11 publications

(2 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In 3D brain-on-a-chip cultures of neurons and astrocytes derived from human induced pluripotent stem cells, exposure to various concentrations of malathion inhibited AChE activity and compromised cell viability, which was rescued by the addition of butyrylcholinesterase [44]. Prenatal exposure to non-AChE inhibiting doses of malathion induced developmental neurotoxicity (memory impairment and spatial learning deficits) in guinea pigs, highlighting the significant impact it has on developmental neurotoxicity [45]. Interestingly, OP mixtures (malathion and chlorpyrifos) exhibited synergistic toxic effects and appeared lethal at concentrations that were sublethal in standalone conditions [46,47].…”

Section: Cell Mechanicsmentioning

confidence: 99%

Phenotypic and Biomechanical Characteristics of Human Fetal Neural Progenitor Cells Exposed to Pesticide Compounds

Sarsfield,

Vasu,

Abuoun

et al. 2023

Biophysica

View full text Add to dashboard Cite

Various forms of pesticides have been reported to be among the environmental toxicants, which are detrimental to human health. The active ingredients of these formulations can enter the human body through air, food, or water. Epidemiological studies suggest that these compounds strongly affect the developing brain in fetal and infant stages due to their ability to breach the underdeveloped blood–brain barrier. Since neural progenitor stem cells (NPCs) in the developing brain are the most vulnerable to these compounds, the mechanisms by which NPCs experience toxicity upon exposure to these chemicals must be investigated. Here, we assessed the viability of human fetal NPCs in 2D cultures in the presence of the active ingredients of six widely used pesticides using Live/Dead® and Hoechst staining. The IC50 values ranged from 4.1–201 μM. A significant drop in cell viability with increasing toxicant concentration (p < 0.01) was noted, with the order of toxicity being malathion < 4-aminopyridine < methoprene < prallethrin < temephos < pyriproxyfen. Changes in cellular biomechanical characteristics (Young’s modulus, tether force, membrane tension, and tether radius) were quantified using atomic force microscopy, whereas cell migration was elucidated over 48 h using a customized wound-healing assay. The Young’s modulus of fetal NPCs exposed to IC50/2 doses of these compounds was reduced by 38–70% and that of those exposed to IC50 doses was reduced by 71–80% (p < 0.001 vs. controls for both; p < 0.01 for IC50 vs. IC50/2 for each compound). Similar patterns were noted for tether forces and membrane tension in fetal NPCs. NPC migration was found to be compound type- and dose-dependent. These results attest to the significant detrimental effects of these compounds on various aspects of the human fetal NPC phenotype, and the utility of cell mechanics as a marker to assess developmental neurotoxicity.

show abstract

Section: Cell Mechanicsmentioning

confidence: 99%

Phenotypic and Biomechanical Characteristics of Human Fetal Neural Progenitor Cells Exposed to Pesticide Compounds

Sarsfield,

Vasu,

Abuoun

et al. 2023

Biophysica

View full text Add to dashboard Cite

show abstract

“…Deformity and cancer can also be brought on by prolonged exposure or excessive MP consumption. 6,7 As a result, careful monitoring of MP pesticide residues in CHM can guarantee both the standardization of MP use in the cultivation and production of CHM as well as their quality and yield.…”

mentioning

confidence: 99%

A Rapid and Easy Procedure of Enzyme Biosensor based on Nitrogen-Doped Graphene for Detection of Methyl Parathion in CHM

Sun,

Yu,

et al. 2024

J. Electrochem. Soc.

View full text Add to dashboard Cite

Methyl parathion (MP), an organophosphorus pesticide that is frequently used to control pests during the planting process of Chinese herbal medicine (CHM), has been used without sufficient control, leading to excessive residues on the surface of CHM, which has a serious impact on the quality and safety of CHM and their preparations. Consequently, it is crucial to carefully control MP during the cultivation, processing, and manufacture of CHM. Based on this, nitrogen-doped graphene (N-Gr) with a high conductivity and chitosan (CS) with good stability were used to modify the glassy carbon electrode (GCE). Subsequently, the prussian blue (PB) with catalytic activity and gold nanoparticles (AuNPs) with excellent biocompatibility were deposited by the electrodeposition method to form the AuNPs/PB/CS@N-Gr/GCE. Then, the acetylcholinesterase (AChE) was effectively immobilized on the electrode surface by covalent bonding between AuNPs and AChE through gold-sulfhydryl bonds. Finally, a rapid and easy procedure of enzyme biosensor (AChE/AuNPs/PB/CS@N-Gr/GCE) for sensitive detection of MP pesticide residues was fabricated. Herein, PB catalyzes the redox reaction of thiocholine, which is produced when acetylthiocholine iodide (ATCHI) undergoes efficient hydrolysis catalyzed by AChE. This process effectively promoted electron transfer, amplifying the sensor’s response signal. After the experimental conditions are optimized, the limit of detection (LOD) for MP is found to be 9.47 × 10−5 μg ml−1. Exhibits a good linear relationship within the concentration range of 1 × 10−3 μg ml−1 ∼ 1 × 101 μg ml−1. Significantly, the fabricated enzyme biosensor excels in swiftly and sensitively detecting trace amounts of MP in real examples. Furthermore, it exhibits robust stability and reproducibility. The excellent performance of this enzyme biosensor not only offers a rapid and easy way to identify and find minute amounts of trace MP pesticide residues in CHM, but also serves as a technical guide for the creation of new, portable, and on-site pesticide residue detection technology for law enforcement.

show abstract

An update of the impact of pesticide exposure on memory and learning

Honatel,

Arbo,

Leal

et al. 2024

Discov Toxicol

View full text Add to dashboard Cite

Learning and memory retention deficits in prepubertal guinea pigs prenatally exposed to low levels of the organophosphorus insecticide malathion

Cited by 11 publications

References 50 publications

Phenotypic and Biomechanical Characteristics of Human Fetal Neural Progenitor Cells Exposed to Pesticide Compounds

Phenotypic and Biomechanical Characteristics of Human Fetal Neural Progenitor Cells Exposed to Pesticide Compounds

A Rapid and Easy Procedure of Enzyme Biosensor based on Nitrogen-Doped Graphene for Detection of Methyl Parathion in CHM

An update of the impact of pesticide exposure on memory and learning

Contact Info

Product

Resources

About