Learning the Trimming Method of Sequence for Removing the Barcodes or Noise

Abstract

Abstract. The initial sequence reads with next-generation sequencing technology have some barcodes or noise, and need to be removed by trimming method of bioinformatics. The trimming method includes trimmer by column with absolute for fixed length reads or percentage for variable length reads and quality trimmer by sliding window. For learning the trimming method of sequence for removing the barcodes or Noise, we do some trimming sequence reads experiments, study the trimmer by column with absolute for fixed length reads or percentage for variable length reads, and compare the results for the different setting of quality score values for the quality trimmer by sliding window.