Lectin attached affinity cryogels for amyloglucosidase adsorption

Bayraktaroğlu, Melis; Orhan, Hande; Evli, Sinem; Akgöl, Sinan; Uygun, Deniz Aktaş; Uygun, Murat

doi:10.1080/07328303.2018.1487972

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Organic monolithic supports are synthesized by the in situ polymerization of a homogenous mixture of individual monomers, which can also act as cross-linkers, usually in the presence of an initiator and a mixture of porogenic solvents. In addition, cryogel monoliths have been functionalized with lectins (Hajizadeh et al, 2012;Uygun et al, 2012;Altunbaş et al, 2013;Krenkova et al, 2015;Bayraktaroğlu et al, 2018Bayraktaroğlu et al, , 2020. Similarly, cryogel monolith synthesis necessitates the polymerization of individual monomers while the reaction is performed at low temperatures in semi-frozen conditions (Lozinsky, 2008).…”

Section: Monolithic Affinity Sorbentsmentioning

confidence: 99%

Recent Advances in Lectin-Based Affinity Sorbents for Protein Glycosylation Studies

Goumenou

Delaunay

Pichon

2021

Front. Mol. Biosci.

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Glycosylation is one of the most significant post-translational modifications occurring to proteins, since it affects some of their basic properties, such as their half-life or biological activity. The developments in analytical methodologies has greatly contributed to a more comprehensive understanding of the quantitative and qualitative characteristics of the glycosylation state of proteins. Despite those advances, the difficulty of a full characterization of glycosylation still remains, mainly due to the complexity of the glycoprotein and/or glycopeptide mixture especially when they are present in complex biological samples. For this reason, various techniques that allow a prior selective enrichment of exclusively glycosylated proteins or glycopeptides have been developed in the past and are coupled either on- or off- line with separation and detection methods. One of the most commonly implemented enrichment methods includes the use of lectin proteins immobilized on various solid supports. Lectins are a group of different, naturally occurring proteins that share a common characteristic, which concerns their affinity for specific sugar moieties of glycoproteins. This review presents the different formats and conditions for the use of lectins in affinity chromatography and in solid phase extraction, including their use in dispersive mode, along with the recent progress made on either commercial or home-made lectin-based affinity sorbents, which can lead to a fast and automated glycosylation analysis.

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Section: Monolithic Affinity Sorbentsmentioning

confidence: 99%

Recent Advances in Lectin-Based Affinity Sorbents for Protein Glycosylation Studies

Goumenou

Delaunay

Pichon

2021

Front. Mol. Biosci.

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“…Results from our groups have already demonstrated lectin attached affinity cryogels for amyloglucosidase adsorption [5], mannose based polymeric nanoparticles for lectin separation [24] and inulinase immobilized lectin affinity cryogels for the high-fructose syrup [25].…”

Section: Temperature Effectmentioning

confidence: 99%

“…Concanavalin A (Con A) is also a metalloprotein containing Mn 2+ and Ca 2+ [16]. Concanavalin A (Con A) has been extensively used in the isolation, fractioning and structural characterization of glycoproteins and other important glycoconjugates carrier glucose and/or mannose residues [3][4][5]. Each monomer contains an independent carbohydrate binding site, for glucose and mannose.…”

Section: Introductionmentioning

confidence: 99%

Lectin Affinity Based Recognition Nanomaterial for Glucose

Kuşat

Kuru

Ulucan

et al. 2020

Hacettepe Journal of Biology and Chemistry

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G lukoz hücreler için önemli bir enerji kaynağıdır ve bir ara / metabolik ajan olarak kullanılır. Glukozun seçici olarak tanınması birçok metabolik hastalığın teşhisi için önemlidir. Bu çalışmada, p(GMA) nanopolimerine lektin ligandı (Con A) bağlanarak p(GMA)-ConA sentezlendi ve karakterize edildi. p(GMA)-ConA nanopolimeri ve glikozun en iyi etkileşiminin 10 mM glukoz konsantrasyonu ve pH = 8.0 koşullarında olduğu belirlenmiştir. Seçicilik analizinde p(GMA)-Con A'nın, glukoz için galaktozdan 2 kat seçici olduğu bulunmuştur. Glukozun tanınması için seçici, yüksek yüzey alanına sahip, düşük maliyetli ve yüksek adsorpsiyon kapasitesiyle yüksek oranda biyouyumlu olan lektin afinite esaslı nanopolimer sistem geliştirilmiştir. Anahtar KelimelerNanoteknoloji, glukoz, lektin afinite kromatografisi, concanavalin A.

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