2013
DOI: 10.1111/rda.12196
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Lectin Histochemistry as a Tool to Identify Apoptotic Cells in the Seminiferous Epithelium of Syrian Hamster (Mesocricetus auratus) Subjected to Short Photoperiod

Abstract: Lectins have been widely used to study the pattern of cellular glycoconjugates in numerous species. In the process of cellular apoptosis, it has been observed that changes occur in the membrane sugar sequences of these apoptotic cells. The aim of our work was to identify which lectins, out of an extensive battery of the same (PNA, SBA, HPA, LTA, Con-A, UEA-I, WGA, DBA, MAA, GNA, AAA, SNA), show affinity for germinal cells in apoptosis, at what stage of cell death they do so and in which germinal cell types the… Show more

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Cited by 17 publications
(18 citation statements)
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“…These groups were established not only according to the time of exposure to the short photoperiod (MR: 4 weeks; SR: 6-8 weeks; TR: 12 weeks) but also to the proportion of tubular sections (control: 0%; MR: 3.9%; SR: 42.9%; TR: 81.6%) in spermatid arrest, tubular diameter (control: 285.3 AE 4.3 lm; MR: 234.3 AE 8.6 lm; SR: 163.7 AE 6.4 lm; TR: 128.9 AE 5.6 lm), and the mean tubular seminiferous epithelium area (control: 55039.9 AE 11254. (Seco-Rovira et al, 2013, 2014.…”
Section: Animals and Samplingmentioning
confidence: 99%
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“…These groups were established not only according to the time of exposure to the short photoperiod (MR: 4 weeks; SR: 6-8 weeks; TR: 12 weeks) but also to the proportion of tubular sections (control: 0%; MR: 3.9%; SR: 42.9%; TR: 81.6%) in spermatid arrest, tubular diameter (control: 285.3 AE 4.3 lm; MR: 234.3 AE 8.6 lm; SR: 163.7 AE 6.4 lm; TR: 128.9 AE 5.6 lm), and the mean tubular seminiferous epithelium area (control: 55039.9 AE 11254. (Seco-Rovira et al, 2013, 2014.…”
Section: Animals and Samplingmentioning
confidence: 99%
“…In the case of aging, germ cell death occurs mainly in the spermatogonia and spermatocytes, whereas in the case of fully regressed testes cell death occurs mainly in spermatocytes (Morales et al, 2007). Such a loss of cells through apoptosis during the epithelium atrophy process seems to affect not only spermatocytes but also other germ cells (Seco-Rovira et al, 2013). Cell loss also includes phagocytosis of round spermatids by Sertoli cells and the death of the latter through apoptosis (Seco-Rovira et al, 2014).…”
Section: Introductionmentioning
confidence: 99%
“…Our results suggest that "the residues of Gal β1,3-GalNAcα1, α-D-mannose, Nacetylgalactosamine or L-fucose in the N-glycoprotein core are involved in apoptosis regardless of the cell type, whereas the L-fucose terminal (Lewis X antigen; LTA)" [55,59] is only involved in the apoptosis of spermatocytes (Figure 1), b) the residues identified by our battery of lectins in apoptotic cells were similar to those found in isolated cell types in apoptosis by other authors: β-D-galactose and α-D-mannose [44,46], L-Fucose (antigen Lewis X) [60] or Nacetylgalactosamine [61] in which the changes we observed in some sugars have been observed in the early stages of apoptosis [44,46,49,62]. These carbohydrates could participate in the identification and removal of apoptotic cells by phagocytes (Sertoli cells), based on their recognition by the 'eat me signals' found on the cell membrane [51].…”
Section: Apoptosis Identification In Seminiferous Epithelium Through mentioning
confidence: 96%
“…Recently our investigation group has used lectin-binding as a tool for the identification of apoptotic cells in seminiferous epithelium of histological sections of hamster testes [55]. In normal conditions, apoptosis in the seminiferous epithelium mainly occurs in the spermatogonia and spermatocytes and, to a lesser degree, in early spermatids [56] thus regulating the amount of germ cells that are supported by the Sertoli cells [57].…”
Section: Apoptosis Identification In Seminiferous Epithelium Through mentioning
confidence: 99%
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