Lectin obtained from the red seaweed Bryothamnion triquetrum: Secondary structure and anti-inflammatory activity in mice

Fontenelle, Thais Pontes Carvalho; Lima, Glauber Cruz; Mesquita, Jacilane Ximenes; Lopes, José Luiz de Souza; Brito, Tarcísio Vieira de; Júnior, Francisco das Chagas Vieira; Sales, Adriano Bezerra; Aragão, Karoline S.; Souza, Marcellus H.; Barbosa, André Luiz dos Reis; Freitas, Ana Lúcia Ponte

doi:10.1016/j.ijbiomac.2018.02.058

Cited by 49 publications

(25 citation statements)

References 60 publications

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“…Inhibition of myeloperoxidase and neutrophil mediated hypochlorous acid formation in vitro and endothelial cell injury by epigallocatechin gallate was also reported (41). Further, the antiinflammatory activity of a lectin extracted from the red seaweed Bryothamnion triquetrum by means of inhibiting myeloperoxidase activity in paw tissue (42). Similarly, inhibitive effect of quercetin on MPO mediated oxidant hypochlorous acid formation or scavenging of the oxidant hypochlorous acid was also evaluated (43).…”

Section: Myeloperoxidase (Mpo) Activitymentioning

confidence: 99%

Enzymes associated with anti-inflammatory potentialities of purified terpenoid extracts from the selected sea weeds

Sumayya¹,

Lubaina²,

Murugan³

2020

IJAM

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Introduction: Macrophages are phagocytic WBCs involved in the immune defense and will be activated during inflammatory disorders. The synthesis of cytokines and mediators, particularly nitric oxide (NO) is triggered by the macrophage activator. NO induces many biological events. Therefore, NO regulation is proven to be potential for exploring anti-inflammatory drugs. Aim: The anti-inflammatory action of the purified terpenoid extracts from red algae such as Hypnea musciformis, Gracilaria dura and Kappaphycus alvarezii on LPS induced RAW 264.7 macrophages on lipoxygenase, cyclooxygenase, hyaluronidase, xanthine oxidase and myeloperoxidase inhibitory effects were evaluated. Methods: The methanolic extract of the sea weeds were subjected to silica gel column chromatography and the fraction was further subjected to GC-MS analysis. Then, the potentiality of the purified terpenoid extracts to inhibit various inflammation causing enzymes such as COX, LOX, hyaluronidase, xanthineoxidase and myeloperoxidase were carried out. Findings: The terpenoid extracts reduced the enzyme activities in a dose dependent manner as compared to control group. The extracts inhibited xanthine oxidase activity effectively at 250 µg/ml i.e., a maximum inhibitory activity of 62.1% as compared to the standard drug, allopurinol. The extract significantly inhibited lipoxygenase activity, with highest inhibitory activity at 100 µg/ml. The nitric acid synthesis was reduced to 8.5 µM by Hypnea musciformis. Conclusion: The present study revealed that the purified terpenoid extracts from H. musciformis exhibited potent anti-inflammatory activities followed by G. dura and K. alvarezii via regulating the anti-inflammatory enzymes. These findings provide justification for the traditional use of the red algae in inflammatory conditions.

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Section: Myeloperoxidase (Mpo) Activitymentioning

confidence: 99%

Enzymes associated with anti-inflammatory potentialities of purified terpenoid extracts from the selected sea weeds

Sumayya¹,

Lubaina²,

Murugan³

2020

IJAM

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“…They are a structurally diverse group of highly specific and reversibly carbohydrate-binding proteins [70]. The three groups of macroalgae (Rhodophyta, Phaeophyta, and Chlorophyta) can produce lectins [71], and these lectins present great potential for the development of new drugs [72][73][74][75][76]. In fact, because of the highly specific way lectins bind to sugars outside cell surfaces inhibiting cell proliferation [77,78], lectins primarily show antiviral, antibacterial, and antifungal activities [73,[79][80][81].…”

Section: Griffithsinmentioning

confidence: 99%

Seaweed Secondary Metabolites with Beneficial Health Effects: An Overview of Successes in In Vivo Studies and Clinical Trials

et al. 2019

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Macroalgae are increasingly viewed as a source of secondary metabolites with great potential for the development of new drugs. In this development, in vitro studies are only the first step in a long process, while in vivo studies and clinical trials are the most revealing stages of the true potential and limitations that a given metabolite may have as a new drug. This literature review aims to give a critical overview of the secondary metabolites that reveal the most interesting results in these two steps. Phlorotannins show great pharmaceutical potential in in vivo models and, among the several examples, the anti-dyslipidemia activity of dieckol must be highlighted because it was more effective than lovastatin in an in vivo model. The IRLIIVLMPILMA tridecapeptide that exhibits an in vivo level of activity similar to the hypotensive clinical drug captopril should still be stressed, as well as griffithsin which showed such stunning results over a variety of animal models and which will probably move onto clinical trials soon. Regarding clinical trials, studies with pure algal metabolites are scarce, limited to those carried out with kahalalide F and fucoxanthin. The majority of clinical trials currently aim to ascertain the effect of algae consumption, as extracts or fractions, on obesity and diabetes.Studies focusing on the preparation of macroalgae extracts and their chemical characterization revealed a large range of seaweed compounds with very interesting biological activities including antitumor, anti-inflammatory, antimicrobial, antidiabetic, antivirus, antihypertensive, fat-lowering, and neuroprotective activities [12][13][14][15].The large volume of studies proving the seaweed compound activities in in vitro systems [16][17][18][19] hints the need for further advancements in the knowledge about macroalgae compound efficiency in living systems (in vivo) and their use in the development of pharmaceuticals. In vitro studies are very relevant and yield very important information, but they only represent the first step of a long process, and the results obtained rarely reveal anything about the effects of a compound in vivo, because the responses observed in vitro can be magnified, diminished, or totally different in a more complex and integrated system. In fact, in vivo studies and clinical trials are those which contribute most to truly understanding the real potential of compounds as future pharmaceuticals.In this regard, the present work intends to present insight into the results obtained in the last few years regarding secondary metabolites, such as phlorotannins, halogenated compounds, fucoxanthin, and fucosterol isolated from macroalgae, involved in in vivo studies and clinical trials, identifying the research opportunities and knowledge gaps, to valorize these compounds and their natural resources. The intention is not to present an exhaustive survey of all published works, but rather a selection of authors based on the following criteria: in-depth studies involving pure compounds most characteristic f...

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“…In addition, it is possible oral use of griffithsin to prevent rectal transmission of HIV. All this makes the griffithsin an effective base for drugs, since lectins have an anti-inflammatory activity too [23].…”

Section: Marine Algae Metabolitesmentioning

confidence: 99%

Marine Algae Metabolites as Promising Therapeutics for the Prevention and Treatment of HIV/AIDS

et al. 2019

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This review presents an analysis of works devoted to the anti-human immunodeficiency virus (HIV) activity of algae metabolites—sulfated polysaccharides (fucoidans, carrageenans), lectins, laminarans, and polyphenols. Despite the presence of a significant number of antiretroviral drugs, the development of new therapeutic and prophylactic agents against this infection remains very urgent problem. This is due to the variability of HIV, the absence of an animal model (except monkeys) and natural immunity to this virus and the toxicity of therapeutic agents and their high cost. In this regard, the need for new therapeutic approaches and broad-spectrum drugs, which in addition to antiviral effects can have anti-inflammatory, antioxidant, and immunomodulatory effects, and to which the minimum resistance of HIV strains would be formed. These requirements meet the biologically active substances of marine algae. The results of experimental and clinical studies conducted in vitro and in vivo are presented, and the issues of the anti-HIV activity of these compounds are considered depending on their structural features. On the whole, the presented data prove the high efficiency of seaweed metabolites and justify the possibility of their use as a potential basis for the development of new drugs with a wide spectrum of activity.

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Lectin obtained from the red seaweed Bryothamnion triquetrum: Secondary structure and anti-inflammatory activity in mice

Cited by 49 publications

References 60 publications

Enzymes associated with anti-inflammatory potentialities of purified terpenoid extracts from the selected sea weeds

Enzymes associated with anti-inflammatory potentialities of purified terpenoid extracts from the selected sea weeds

Seaweed Secondary Metabolites with Beneficial Health Effects: An Overview of Successes in In Vivo Studies and Clinical Trials

Marine Algae Metabolites as Promising Therapeutics for the Prevention and Treatment of HIV/AIDS

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