Leech extract: A candidate cardioprotective against hypertension-induced cardiac hypertrophy and fibrosis

Wang, Chien-Hao; Pandey, Sudhir; Sivalingam, Kalaiselvi; Shibu, Marthandam Asokan; Kuo, Wei‐Wen; Yu-LanYeh,; Viswanadha, Vijaya Padma; Lin, Yuan-Chuan; Liao, Shih-Chieh; Huang, Chih‐Yang

doi:10.1016/j.jep.2020.113346

Cited by 20 publications

(15 citation statements)

References 31 publications

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“…Increase in both DAP and SAP correlates with higher CH when S and F are consumed in combination. A direct relationship in the development of CH by AH-induced pressure overload has been widely reported [30]. It has been described that children with obesity, and with obesity plus AH, had CH, indicating that CH is not only present in adulthood, but also at early ages, and that its development is independently favored by AH and obesity [31].…”

Section: Discussionmentioning

confidence: 92%

Consumption of combined fructose and sucrose diet exacerbates oxidative stress, hypertrophy and CaMKIIδ oxidation in hearts from rats with metabolic syndrome

et al. 2022

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The prevalence of the metabolic syndrome (MetS) and its cardiac comorbidities as cardiac hypertrophy (CH) have increased considerably due to the high consumption of carbohydrates, such as sucrose and/or fructose. We compared the effects of sucrose (S), fructose (F) and their combination (S + F) on the development of MetS in weaned male Wistar rats and established the relationship between the consumption of these sugars and the degree of cardiac CH development, oxidative stress (OS) and Calcium/calmodulin-dependent protein kinase type II subunit delta oxidation (ox-CaMKII δ ). 12 weeks after the beginning of treatments with S, F or S + F, arterial pressure (AP) was measured and 8 weeks later (to complete 20 weeks) the animals were sacri ced and blood samples, visceral adipose tissue and hearts were obtained. Biochemical parameters were determined in serum and cardiac tissue to evaluate the development of MetS and OS. To evaluate CH, atrial natriuretic peptide (ANP), CaMKII δ and ox-CaMKII δ were determined by western blot and histological studies were performed in cardiac tissue. Our data showed that chronic consumption of S + F exacerbates MetS-induced CH which is related with a higher OS and ox-CaMKII δ .

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Section: Discussionmentioning

confidence: 92%

Consumption of combined fructose and sucrose diet exacerbates oxidative stress, hypertrophy and CaMKIIδ oxidation in hearts from rats with metabolic syndrome

et al. 2022

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“…The tissue staining was performed following the previous methods (Wang et al., 2021). Briefly, the deparaffinized sections were boiled for 15 min in citrate buffer and were then blocked in blocking buffer (2.5% horse serum in PBS‐Tween 20) for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…Finally, the slides were rinsed with PBS, air‐dried, mounted using VectaMount (H‐5000), and imaged using a microscope (Olympus). For Mason's trichrome staining to find the level of fibrotic lesions was performed by following previously reported methods (Wang et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

Glycyrrhiza uralensis root extract ameliorates high glucose‐induced renal proximal tubular fibrosis by attenuating tubular epithelial‐myofibroblast transdifferentiation by targeting TGF‐β1/Smad/Stat3 pathway

Lin

Paul

Kuo

et al. 2022

Journal of Food Biochemistry

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Growing evidences indicate that high glucose toxicity-associated fibrotic effects play a pivotal role in diabetic nephropathy (DN). Tubular epithelial-myofibroblast transdifferentiation is a major hallmark of renal fibrosis event under diabetic stress. Roots of Glycyrrhiza uralensis (Radix glycyrrhizae) used as a sweetener and traditional Chinese medicine possess high potential for renal protection. In this study, a cell model for high glucose (HG) injury with HK-2 renal proximal tubular epithelial cell line and a type-II-diabetes model with Apoe em1/Narl /Narl mice was established and the beneficial effects of aqueous R. glycyrrhizae extract (RGE) was investigated. RGE-induced regulation on the high glucose-induced excessive production of TGF-β1 and the Smad/ Stat3 mechanisms of renal fibrosis were determined. HK-2 cells were challenged with

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“…H. nipponia as a traditional Chinese medicine contains hirudin which is the strongest thrombin natural inhibitor found so far ( Straub et al, 2011 ; Liu et al, 2019 ) and it has unique properties of hypolipidemic, anti-tumor, anti-fibrotic, and anti-inflammatory. Furthermore, it has widely been used clinically to treat a variety of diseases such as hypertension, hyperlipidemia, cerebral infarction and malignancies ( Sig et al, 2017 ; Pickrell et al, 2020 ; Wang et al, 2021 ). In addition, leeches have the ability to self-repair damage to the central nervous system and restore function, and are often used as model organisms in the field of neurobiology ( Tasiemski and Salzet, 2017 ; Meiser et al, 2019 ; Heath-Heckman et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have focused on the role of effective components in leech and their cultivation ( Iwama et al, 2019 ; Wang et al, 2021 ). Recent advancements in high-throughput technologies provide an opportunity to dig out in-depth and comprehensive high quality leech genomes.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Sequencing Analysis of Hirudo nipponia in Different Growth Periods

Yan

et al. 2022

Front. Physiol.

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Hirudo nipponia is the only blood-sucking leech included in Chinese Pharmacopoeia having distinct features of anticoagulation, exorcizing blood stasis, and promoting menstruation. Despite such significant characteristics, very little is known about its molecular genetics and related physiological mechanisms. In this study, the transcriptomes of H. nipponia at three developmental stages (larvae, young, and adults), revealed a total of 1,348 differentially expressed genes (DEGs), 223 differentially expressed lncRNAs, and 88 novel mRNAs. A significant diverse gene expression patterns were observed at different developmental stages which were analyzed by differential gene expression trends, and the overall gene expression trends consist of three overall down-regulated trends, and two overall up-regulated trends. Furthermore, the GO and KEGG enrichment functional annotation analysis revealed that these DEGs were mainly associated with protein hydrolysis, signal transduction, energy metabolism, and lipid metabolism while growth, development, metabolism, and reproduction-related DEGs were also found. Additionally, real-time quantitative PCR results confirmed deep sequencing results based on the relative expression levels of nine randomly selected genes. This is the first transcriptome-based comprehensive study of H. irudo nipponia at different developmental stages which provided considerable deep understanding related to gene expression patterns and their relevant developmental pathways, neurodevelopmental and reproductive characteristics of the leech.

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Leech extract: A candidate cardioprotective against hypertension-induced cardiac hypertrophy and fibrosis

Cited by 20 publications

References 31 publications

Consumption of combined fructose and sucrose diet exacerbates oxidative stress, hypertrophy and CaMKIIδ oxidation in hearts from rats with metabolic syndrome

Consumption of combined fructose and sucrose diet exacerbates oxidative stress, hypertrophy and CaMKIIδ oxidation in hearts from rats with metabolic syndrome

Glycyrrhiza uralensis root extract ameliorates high glucose‐induced renal proximal tubular fibrosis by attenuating tubular epithelial‐myofibroblast transdifferentiation by targeting TGF‐β1/Smad/Stat3 pathway

Comparative Transcriptome Sequencing Analysis of Hirudo nipponia in Different Growth Periods

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