LEI0258 microsatellite variability and its relationship to B-F haplotypes in Brazilian (blue-egg Caipira) chickens

Lima-Rosa, Carlos André da Veiga; Canal, Cláudio Wageck; Fallavena, Paulo Roberto Vargas; Freitas, Loreta B.; Salzano, Francisco M.

doi:10.1590/s1415-47572005000300008

Cited by 25 publications

(21 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The evidence of genetic variability in Brazilian (blue-egg Caipira) chickens is one way to demonstrate the importance of conserving these animals for preservation of species diversity. This diversity has been reported by Lima-Rosa et al (2004) and Lima-Rosa et al (2005) when they studied a microsatellite locus (LEI0258) and two genes of immune system (B-F genes), in this same population. However, it is necessary to analyze a greater number of loci to obtain a further characterize of this variability.…”

Section: Introductionsupporting

confidence: 70%

Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens

et al. 2014

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to investigate the genetic polymorphism of fifteen microsatellites loci in Brazilian (blue-egg Caipira) chickens. Samples were collected from 100 blue eggs of Caipira chickens from rural properties in the city of Dois Lajeados, RS. After DNA extraction, the fragments related to molecular markers LEI0248, LEI0221, LEI0214, LEI0192, LEI0217, LEI0254, LEI0194, LEI0212, MCW0371, ADL0278, LEI0234, MCW0183, MCW0216, MCW0330 and MCW0081 were obtained by polymerase chain reaction (PCR). The statistical analysis were carried out with the softwares ARLEQUIN 3.5 version and CER-VUS 3.0.3 version. The allelic and genotypic frequencies, deviations from Hardy-Weinberg equilibrium, estimates of observed (HO) and expected (HE) heterozygosity and polymorphic information content (PIC) were obtained for each marker locus. A total of 186 alleles from 15 loci were obtained, with sizes ranging of 83 to 490 base pairs. The medium number of alleles was 12.4, the HE was 0.76±0.14 and HO was 0.49±0.21 and PIC was 0.706. The first conclusion is that the microsatellites used are polymorphic and can be used to genetic studies in chickens. The second is that the "Caipira" chicken (blue eggs) population investigated has a great genic variability, which makes than an important source of genetic resources for future animal breeding programs. Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens nhas caipiras de ovos azuis analisada apresenta grande variabilidade gênica, o que as torna uma importante fonte de recursos genéticos, e que poderão, assim, serem utilizadas em futuros programas de melhoramento genético animal.

show abstract

Section: Introductionsupporting

confidence: 70%

Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…To our surprise allele 357, serologically defined as B21 which is strongly associated with Marek's disease resistance (Briles et al, 1977, Bacon et al, 2001Fulton et al, 2006) was present only in Kadaknath population and absent in all others in the current investigation. This is in contrast to the earlier reports that this allele was absent in Chinese, Tanzanian, Vietnamese and Brazilian chicken populations (Lwelamira et al, 2009;Lima-Rosa et al, 2005;Schou et al, 2007). Although the association of LEI0258 microsatellite alleles with serologically defined haplotypes have earlier been demonstrated in North American and European populations (Fulton et al, 2006), these associations may vary in different populations and needs to be established separately.…”

Section: Resultscontrasting

confidence: 87%

“…Similaly higher heterozygosity has also been reported for Tanzanian, Vietnamese (Lwelamira et al, 2009) and (Iranian (Nikbakht et al, 2013) native chicken populations. However, Aseel population showed relatively lower heterozygosity similar to that of Brazilian chickens (Lima-Rosa et al, 2005). Both the native chicken breeds have been collected from different environment and have undergone only few generations of random mating.…”

Section: Resultsmentioning

confidence: 94%

“…The microsatellite marker LEI0258 is physically located within MHC, between the BG and BF regions (McConnell et al, 1999). Initially Lima-Rosa et al (2005) described the linkage of LEI0258 with chicken B-F haplotypes and subsequently, Fulton et al (2006) demonstrated 26 alleles distinctive of LEI0258 and showed their association with serologically defined MHC haplotypes. In recent times use of this marker for genetic diversity analysis in various chicken populations has been reinforced by many workers (Izadi et al, 2011;Nikbakht et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity analysis of the major histocompatibility complex (MHC) region in Indian native chicken breeds and pureline chickens using the LEI0258 microsatellite marker

Kannaki

Reddy

Chatterjee

2017

IJAR

View full text Add to dashboard Cite

The chicken major histocompatibility complex (MHC) plays an important role in immune response and is strongly associated with resistance and susceptibility to many diseases. The microsatellite marker LEI0258 is physically located within MHC, between the BG and BF regions and can identify serologically well-defined MHC haplotypes. In the present study, a total of 150 samples from pureline chicken breeds including Indian native chicken breeds Aseel (20), Kadaknath (45), White Leghorn (IWI-20; IWK-23), Dahlem red (DR-20), Broiler (PB1-22) was investigated by using LEI0258 microsatellite marker. Overall 22 differently sized alleles (182-552 bp) were identified in all the five lines. Our results indicate that Indian native chicken breeds Aseel (Na=10) and Kadaknath (Na=11) harbored more alleles compared to those of pureline populations (Na=5-6). Analysis of Hardy-Weinberg's equilibrium (HWE) revealed that two populations IWI and Kadaknath deviated from the HWE (P<0.001). LEI0258 microsatellite based MHC haplotyping would be useful in selecting birds for breeding programs and also in genetic resource conservation.

show abstract

“…Although MHC variations have been studied in some commercial flocks (e.g., Fulton et al, 2006), not much is known about MHC variations in noncommercially derived flocks, particularly those commonly used for free-run, free-range, and organic production, except for Lima-Rosa et al (2005), Schou et al (2006), and Lwelamira et al (2008). Noncommercial free-run and free-range chickens are likely to have been exposed to more parasite and disease vectors compared with the commercial breeds, which are bred and reared in indoor cage confinement.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the major histocompatibility complex region in commercial and noncommercial chicken flocks using the LEI0258 microsatellite marker

Izadi¹,

Ritland

Cheng³

2011

Poultry Science

View full text Add to dashboard Cite

Microsatellite marker LEI0258 was used as an indicator to examine the variability of the major histocompatibility complex (MHC) region in 2 commercial layer flocks, 1 experimental layer cross, and 5 noncommercial flocks (used for free-run and free-range meat and egg production). We hypothesized that the populations from noncommercial sources may have more diversity in MHC genes than that in the commercial-source populations. Two related parameters, heterozygosity and the number of alleles harbored by a population, were used to assess the genetic variability. The different combinations of the 22 alleles created 66 genotypes in the 8 chicken populations that were studied. The noncommercial populations, except for the Silkies (SK), harbored more alleles than those in the 2 commercial populations, Lohmann Brown and Lohmann White. The observed heterozygosity of the MHC region was high in all of the populations, except for SK. Considering the 2 parameters we have examined, we can generalize that the intensively selected commercial egg-layer varieties seem to have less genetic variability in their MHC regions compared with that of the noncommercial flocks, which are less intensively selected. The LEI0258 variants can be used as markers to detect most of the MHC haplotypes, but in the different populations the same allele size may not always be associated with the same serologically defined haplotype. The information obtained from this study will be useful for genetic resource conservation and the development of breeding stocks that are suitable for free-range production.

show abstract

LEI0258 microsatellite variability and its relationship to B-F haplotypes in Brazilian (blue-egg Caipira) chickens

Cited by 25 publications

References 16 publications

Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens

Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens

Genetic diversity analysis of the major histocompatibility complex (MHC) region in Indian native chicken breeds and pureline chickens using the LEI0258 microsatellite marker

Genetic diversity of the major histocompatibility complex region in commercial and noncommercial chicken flocks using the LEI0258 microsatellite marker

Contact Info

Product

Resources

About