Lentinan Inhibits Tumor Progression by Immunomodulation in a Mouse Model of Bladder Cancer

Abstract

Background: Lentinan (LNT), an isolated traditional Chinese herbal component, has antitumor potential. In the current study, the intrinsic mechanism of LNT-induced immunity against bladder cancer was explored in a mouse model. Methods: In the mouse model of bladder cancer, we used flow cytometry to detect the LNT caused population changes of T cells, macrophages, MDSC cells, and Treg cells. ELISA was used to evaluate cytokines expression in the supernatant of splenocytes. Results: We found that the administration of LNT increased the proportions of CD3+CD4+ and CD3+CD8+ T cell subsets as well as CD11b+F480+ macrophages, whereas it diminished the subpopulations of CD4+CD25+Foxp3+ regulatory T cells (Tregs) and Gr-1+CD11b+ myeloid-derived suppressor cells (MDSCs). LNT also upregulated the expression of interferon (IFN)-γ and interleukin (IL)-12, accompanied by a significant reduction in IL-10 and tumor growth factor (TGF)-β ( P < .05). Our research further confirmed the synergy between LNT and gemcitabine (GEM) to activate immunity and inhibit the growth of bladder tumors in mouse model. Conclusions: LNT induced macrophage activation, followed by the enhanced proliferation of CD4+ and CD8+ T cells, and the upregulated expression of IFN-γ and IL-2. Meanwhile, the proportions of MDSCs and Tregs were downregulated, leading to a reduced expression of the anti-inflammatory cytokines IL-10 and TGF-β. The synergy between LNT and GEM provides additional evidence supporting the application of this traditional Chinese herbal component for bladder cancer therapy.

Hypoglycemic effect of a novel polysaccharide fromLentinus edodeson STZ-induced diabetic miceviametabolomics study and Nrf2/HO-1 pathway

“…Lentinus edodes, a genus of fungi in the family of Pleurotaceae and class Basidiomycetes, is an edible and medicinal fungus with a long history. Polysaccharides are the main components in Lentinus edodes, and many studies have shown that Lentinus edodes polysaccharides have various pharmacological effects, including anti-tumor, immune-modulatory, hypoglycemic activity, [15][16][17][18] and so on. Lentinan (β-glucan) has been demonstrated to improve insulin sensitivity and restore pancreatic tissue function, thereby leading to enhanced insulin generation from functional β cells and reduced blood glucose levels.…”

Anti-Influenza Effect and Mechanisms of Lentinan in an ICR Mouse Model

“…As IAV continues to evolve and mutate, new drug-resistant strains are emerging, making the development of new anti-influenza medications critical. LNT has received much attention in recent years, and the safety of LNT in food has been proven ( Sun et al., 2020 ). LNT has a variety of actions, including immunomodulatory, anti-inflammatory, antitumor, and antiviral activities, according to recent research ( Han et al., 2001 ; Ren et al., 2019 ; Gu et al., 2022 ; Liu et al., 2022 ).…”

Lentinan induces apoptosis of mouse hepatocellular carcinoma cells through the EGR1/PTEN/AKT signaling axis

“…As the first medicinal macrofungal polysaccharide drug to enter the field of modern biotechnology (5), its unique triple-helix conformation and antitumor activity have particularly attracted attention (6). Several studies have shown that LNT can mediate anti-tumor effects directly and indirectly (7)(8)(9)(10). A previous study found that LNT can exert inhibitory effects in a mammary-specific polyomavirus middle T antigen overexpression mouse model of spontaneous breast cancer (7).…”