Les convertases des prohormones et le système nerveux

Marcinkiewicz, M.; Seidah, Nabil G.; Chretien, M.

doi:10.4267/10608/2961

Cited by 7 publications

(3 citation statements)

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“…The colocalization of PC1 and PC2 with POMC-derived peptides, adrenocorticotropin and ␣-melanotropin in the pituitary gland (Marcinkiewicz et al, 1993a) and with insulin in pancreatic islets (Marcinkiewicz et al, 1994) has been well established, providing a better understanding of their physiologic roles in vivo. With respect to neurotrophic factors, distribution studies (Marcinkiewicz et al, 1993b predicted a role of some PCs in biosynthesis of these factors. The capacity of PCs to process precursors such as pro-NGF (Seidah et al, 1996a), pro-brain-derived neurotrophic factor (pro-BDNF), and proneurotrophin-3 (pro-NT3; Seidah et al, 1996b) into their active end-products has been demonstrated by co-expression in cell lines.…”

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Nerve growth factor and proprotein convertases furin and PC7 in transected sciatic nerves and in nerve segments cultured in conditioned media: Their presence in Schwann cells, macrophages, and smooth muscle cells

Marcinkiewicz

Chen

et al. 1999

J. Comp. Neurol.

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Synthesis of proteins such as nerve growth factor (NGF) is induced after nerve lesion. The NGF precursor (pro‐NGF) requires a posttranslational processing by proprotein convertases to become active. In this report, we re‐examine the localization of NGF protein and mRNA in injured nerve and show that the candidate pro‐NGF convertases furin and PC7 colocalize with NGF in non‐neuronal cells in nerve. By Northern blot analysis, 1.5‐kb and 1.3‐kb NGF mRNAs were shown to be increased in distal and immediately proximal nerve segments on days 1, 4, and 14 after lesion; by Western blot analysis, NGF proteins of high molecular weight were detected after injury. In vivo, two phases of NGF immunopositivity were observed, in macrophages and perivascular cells shortly after lesion and in endoneurial cells on day 1 and 4. To identify the cells containing NGF, nerve segments were incubated in serum‐containing medium with or without conditioning by white blood cells isolated from the circulation. Both hybridization and immunoreactivity signals for NGF were elevated after incubation of nerve segments for 4 hours in conditioned media, so that cells with NGF immunoreactivity could be identified by antibodies to specific cell markers. In these nerve fragments, Schwann cells, perivascular smooth muscle cells, and macrophages contained NGF immunoreactivity. The concentration of furin and PC7 mRNA also increased in lesioned nerves. By immunocytochemical investigation of nerve explants, furin and PC7 were detected in endoneurial cells, macrophages and perivascular cells and were colocalized with NGF. These in vitro and in vivo findings suggest that both furin and PC7 are associated with NGF in several cell types of the sciatic nerve and, hence, may be implicated in intracellular processing of pro‐NGF. J. Comp. Neurol. 403:471–485, 1999. © 1999 Wiley‐Liss, Inc.

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Nerve growth factor and proprotein convertases furin and PC7 in transected sciatic nerves and in nerve segments cultured in conditioned media: Their presence in Schwann cells, macrophages, and smooth muscle cells

Marcinkiewicz

Chen

et al. 1999

J. Comp. Neurol.

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“…Le gène codant l'ostéocrine est exprimé presque exclu- NOUVELLES MAGAZINE sivement par les ostéoblastes [1] et les cellules musculaires [2][3][4]. L'ostéocrine est une protéine hautement conservée entre les espèces (Figure 1) et l'examen de sa structure primaire met en évidence des motifs rappelant ceux entrant dans la composition de plusieurs prohormones [5]. En effet, nous avons démontré par immunobuvardage Western (Western blot) qu'il est possible que des fragments d'ostéocrine soient produits sous l'action de protéases qui reconnaissent des motifs à caractères dibasiques.…”

Section: L'identification D'une Nouvelle Molécule Osseuseunclassified

L’ostéocrine, une nouvelle molécule de signalisation osseuse

Moffatt¹

2008

Med Sci (Paris)

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“…La pro-enképhaline est quant à elle retrouvée dans les lymphocytes T et les monocytes [6]. Au niveau du système nerveux, ces précurseurs subissent une maturation sous l'effet de pro-hormones convertases (PC) spécifiques [7]. Certaines de ces enzymes telles que la PC3 sont co-localisées avec la proenképhaline dans les macrophages humains [8] …”

Section: Implication Des Peptides Opioïdes Dans La Réponse Immunitaireunclassified