Les difficultés d'interprétation des contrôles microbiologiques environnementaux

Barbut, Frédéric; Neyme, Denis

doi:10.1016/s1773-035x(06)80192-9

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…e surfaces are contaminated by microorganisms derived from the patient himself or sedimentation of particles in the air [30]. Several pathogens can survive for days and months in dry surface and also can be considered as the source of HAI [31].…”

Section: Discussionmentioning

confidence: 99%

Infectious Risk of the Hospital Environment in the Center of Morocco: A Case of Care Unit Surfaces

Jaouhar

Lalami

Ouarrak³

et al. 2020

Scientifica

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Background. Equipment and hospital surfaces constitute a microbial reservoir that can contaminate hospital users and thus create an infectious risk. The aim of this work, which was carried out for the first time at a hospital in Meknes (regional hospital in the center of Morocco), is to evaluate the microbiological quality of surfaces and equipment in three potential risk areas (burn unit, operating room, and sterilization service). Methods. This study was carried out over a period of 4 months (February–May 2017). A total of 60 samples were taken by swabbing according to the standard (ISO/DIS 14698-1 (2004)) in an environment of dry area and equipment after biocleaning. Isolation and identification were performed according to conventional bacteriological methods and by microscopic observation for fungi. Results. The study showed that 40% of surface samples were contaminated after biocleaning. The burn unit recorded a percentage of 70% contamination (p value <0.001), 13% for the sterilization service, and 7% for the operating room. 89% of the isolates were identified as Gram-positive bacteria against 11% for fungi (p value <0.001). Bacterial identification showed coagulase-negative staphylococci (32%), Bacillus spp. (16%), Corynebacterium (8%), and oxidase-negative Gram-positive bacillus (40%) while fungal identification showed Aspergillus niger (n = 2) and Aspergillus nidulans (n = 1). Conclusion. To control the infectious risk related to equipment and hospital surfaces, it would be necessary to evaluate the disinfection protocol applied in these units.

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Section: Discussionmentioning

confidence: 99%

Infectious Risk of the Hospital Environment in the Center of Morocco: A Case of Care Unit Surfaces

Jaouhar

Lalami

Ouarrak³

et al. 2020

Scientifica

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“…The hospital environment is exposed to different types of contamination, especially by resistant microorganisms, including bacteria, which increases the risk of infection to immunocompromised patients [25,26]. Disinfection represents the main strategy against pathogenic or potentially pathogenic microorganisms in the hospital [27].…”

Section: Discussionmentioning

confidence: 99%

Susceptibility patterns of bacteria isolated from the hospital environment towards disinfectants commonly used for surfaces and medical devices

Jaouhar

Lalami²,

Bouzid

et al. 2021

E3S Web Conf.

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This study aimed to evaluate the bactericidal activity of common disinfectants used for surfaces and medical devices. Sodium hypochlorite (D1), disinfectant (D2) composed of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, chloride de didecyldimethylammonium, and disinfectant (D3) composed of Didecyldimethylammonium chloride and Polyhexamethylene biguanide hydrochloride, were tested against 15 strains isolated from the hospital environment and four reference bacteria. The microdilution method was performed to assess antimicrobial activity. The susceptibility was evaluated by comparing the minimum inhibitory dilution with the dilution of disinfectant recommended by the manufacture. D1 and D2 were active against Staphylococcus epidermidis, Staphylococcus saprophyticus, Enterobacter cloacae, Escherichia coli, Pseudomonas fluorescens, Methicillin-resistant Staphylococcus aureus, Bacillus spp, Corynebacterium spp, Gram-positive bacillus, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 3366, and Pseudomonas aeruginosa ATCC 27853 strains but not active against Micrococcus spp, and Staphylococcus aureus ATCC 29213. D3 was ineffective against Micrococcus spp, Bacillus Gram Positive, Staphylococcus epidermidis, and Escherichia coli ATCC 25922. Therefore, D1 and D2 can eliminate most pathogenic bacteria in hospitals, in comparison to D3. It is necessary to monitor the antibacterial activity of disinfectants against reference strains but also against those usually present on surfaces. The obtained results could have promising applications in controlling the emergence of nosocomial infections.

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“…The media are supplemented with 50 μg / mL amphotericin B as an antifungal agent. The dishes are incubated at 28 °C for 3 weeks or more ( Barbut and Neyme, 2006 , Goodfellow, 2010 , Elbendary et al, 2018 ).The colonies having the morphological characteristics of actinomycetes are purified on ISP2, and then frozen at −20 °C (The culture medium used for the cryoconservation is nutrient broth supplemented with 15% glycerol as a cryoprotectant which reduces freezing damage by increasing viscosity. Instead of intercellular and extracellular water crystallizing, the syrupy solution becomes an amorphous ice by vitrifying).…”

Section: Methodsmentioning

confidence: 99%

Biocontrol of toxinogenic Aspergillus flavus and Fusarium oxysporum f. sp. albedinis by two rare Saharan actinomycetes strains and LC-ESI/MS-MS profiling of their antimicrobial products

Meliani

Makhloufi

Chérif

et al. 2022

Saudi Journal of Biological Sciences

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Les difficultés d'interprétation des contrôles microbiologiques environnementaux

Cited by 7 publications

References 15 publications

Infectious Risk of the Hospital Environment in the Center of Morocco: A Case of Care Unit Surfaces

Infectious Risk of the Hospital Environment in the Center of Morocco: A Case of Care Unit Surfaces

Susceptibility patterns of bacteria isolated from the hospital environment towards disinfectants commonly used for surfaces and medical devices

Biocontrol of toxinogenic Aspergillus flavus and Fusarium oxysporum f. sp. albedinis by two rare Saharan actinomycetes strains and LC-ESI/MS-MS profiling of their antimicrobial products

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