Lessons from the Embryonic Neural Stem Cell Niche for Neural Lineage Differentiation of Pluripotent Stem Cells

Solozobova, Valeriya; Wyvekens, Nicolas; Pruszak, Jan

doi:10.1007/s12015-012-9381-8

Cited by 44 publications

(49 citation statements)

References 171 publications

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“…Large-scale generation of a specific neural subtype also remains a major challenge for neuronal differentiation of hPSCs. Recreating the stem cell niches enriched with ECMs is being pursued to address these challenges [15] .…”

Section: Pluripotent Stem Cell-derived Neural Progenitor Cellsmentioning

confidence: 99%

“…Differentiating hPSCs into OPCs or neural progenitor cells (NPCs) with a high purity has been demonstrated, but their function and maturation are still under investigation [12][13][14] . Extracellular matrix (ECM) plays an important role in neural differentiation of hPSCs and the maturation of the derived neural cells [15] . ECM proteins, through the interaction with integrins expressed on the neural cells, modulate cell survival, migration, proliferation, and the differentiated cell function [16] .…”

Section: Introductionmentioning

confidence: 99%

“…Besides ECM composition, the mechanical property of ECMs is also found to regulate neural lineage commitment of hPSCs recently. To better understand the in vivo development and the "niches", i.e., microenvironment, of neural tissue development [15] , three-dimensional (3-D) ECMs, both natural and synthetic, have been investigated for efficient neural differentiation of hPSCs. 3-D ECM scaffolds provide not only physical support for cell adhesion, but also the structural and biomechanical cues that can be transduced into biochemical signals, affecting cellular composition during neural differentiation [17,18] .…”

Section: Introductionmentioning

confidence: 99%

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Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Liu

Yan

et al. 2014

WJSC

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Neural cells differentiated from pluripotent stem cells (PSCs), including both embryonic stem cells and induced pluripotent stem cells, provide a powerful tool for drug screening, disease modeling and regenerative medicine. High-purity oligodendrocyte progenitor cells (OPCs) and neural progenitor cells (NPCs) have been derived from PSCs recently due to the advancements in understanding the developmental signaling pathways. Extracellular matrices (ECM) have been shown to play important roles in regulating the survival, proliferation, and differentiation of neural cells. To improve the function and maturation of the derived neural cells from PSCs, understanding the effects of ECM over the course of neural differentiation of PSCs is critical. During neural differentiation of PSCs, the cells are sensitive to the properties of natural or synthetic ECMs, including biochemical composition, biomechanical properties, and structural/topographical features. This review summarizes recent advances in neural differentiation of human PSCs into OPCs and NPCs, focusing on the role of ECM in modulating the composition and function of the differentiated cells. Especially, the importance of using three-dimensional ECM scaffolds to simulate the in vivo microenvironment for neural differentiation of PSCs is highlighted. Future perspectives including the immediate applications of PSC-derived neural cells in drug screening and disease modeling are also discussed.© 2014 Baishideng Publishing Group Co., Limited. All rights reserved.Key words: Pluripotent stem cells; Neural differentiation; Extracellular matrix; Three-dimensional; Drug screening Core tip: Neural cells derived from human pluripotent stem cells (hPSCs), including oligodendrocyte progenitor cells and neural progenitor cells, emerge as an unlimited and physiologically relevant cell source for drug screening, disease modeling, and regenerative medicine. Natural and synthetic extracellular matrices play an important role in regulating neural differentiation, cell migration, and the derived neural cell maturation. Recent advances in neural differentiation of hPSCs on extracellular matrices in 2-D and 3-D systems are reviewed in this paper. The immediate applications of the derived neural cells in drug screening and disease modeling are also discussed.

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Section: Pluripotent Stem Cell-derived Neural Progenitor Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Liu

Yan

et al. 2014

WJSC

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“…In the stem cell microenvironment, ECM components are capable of trapping growth factors and regulate their local concentrations and availability [120].…”

Section: Interactions Between Ecm and Growth Factorsmentioning

confidence: 99%

Dental Pulp Stem Cell Niche

Jamal

Garcı́a-Godoy

et al. 2015

Tissue-Specific Stem Cell Niche

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“…A recent publication has exploited the midbrain floorplate precursor marker CORIN 34,35 to enrich for dopaminergic precursors in Parkinson cell transplantation paradigms 36 . CD molecules are not only markers, but functionally relevant mediators of cell-cell interactions and of a cell's ability to respond to cues from extracellular matrix molecules and growth factors 37 . One strategy of further enhancing the arsenal of combinatorial CD antigens to characterize neural lineage development is to use known intracellular markers to screen for and define CD antigen combinations for a particular cell type of interest.…”

Section: Introductionmentioning

confidence: 99%

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Menon

Thomas

et al. 2014

JoVE

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Flow cytometry has been extensively used to define cell populations in immunology, hematology and oncology. Here, we provide a detailed description of protocols for flow cytometric analysis of the cluster of differentiation (CD) surface antigens and intracellular antigens in neural cell types. Our step-by-step description of the methodological procedures include: the harvesting of neural in vitro cultures, an optional carboxyfluorescein succinimidyl ester (CFSE)-labeling step, followed by surface antigen staining with conjugated CD antibodies (e.g., CD24, CD54), and subsequent intracellar antigen detection via primary/secondary antibodies or fluorescently labeled Fab fragments (Zenon labeling). The video demonstrates the most critical steps. Moreover, principles of experimental planning, the inclusion of critical controls, and fundamentals of flow cytometric analysis (identification of target population and exclusion of debris; gating strategy; compensation for spectral overlap) are briefly explained in order to enable neurobiologists with limited prior knowledge or specific training in flow cytometry to assess its utility and to better exploit this powerful methodology. Video LinkThe video component of this article can be found at

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Lessons from the Embryonic Neural Stem Cell Niche for Neural Lineage Differentiation of Pluripotent Stem Cells

Cited by 44 publications

References 171 publications

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Neural differentiation from pluripotent stem cells: The role of natural and synthetic extracellular matrix

Dental Pulp Stem Cell Niche

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

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