Let-7c miRNA Inhibits the Proliferation and Migration of Heat-Denatured Dermal Fibroblasts Through Down-Regulating HSP70

Jiang, Tao; Wang, Xingang; Wu, Weiwei; Zhang, Fan; Wu, Shifeng

doi:10.14348/molcells.2016.2336

Cited by 15 publications

(4 citation statements)

References 35 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the keloid fibroblasts, HSP70 was also proved to be overexpressed [30]. Moreover, approximately 5-fold increase in HSP70 at mRNA level was demonstrated in fibroblasts upon heat shock [31]. Furthermore, in an earlier research, HSP70 was proved to promote cell proliferation and migration in renal cell carcinoma cells [32].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-16 inhibits cell proliferation and migration by targeting heat shock protein 70 in heat-denatured dermal fibroblasts

Zhang

Dai

Fan

et al. 2019

Korean J Intern Med

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

MicroRNA-16 inhibits cell proliferation and migration by targeting heat shock protein 70 in heat-denatured dermal fibroblasts

Zhang

Dai

Fan

et al. 2019

Korean J Intern Med

View full text Add to dashboard Cite

show abstract

“…Primary HDFs were derived from normal human skin collected from patients who had undergone plastic surgery procedures in the Department of Burns and Plastic Surgery, as previously described ( 24 ). Informed consent was obtained from the participants and approval was provided by the Ethics Committee of the College of Clinical Medicine of Henan University.…”

Section: Methodsmentioning

confidence: 99%

EZH2-mediated suppression of lncRNA-LET promotes cell apoptosis and inhibits the proliferation of post-burn skin fibroblasts

Zheng

2018

Int J Mol Med

View full text Add to dashboard Cite

Although the upregulation of enhancer of zeste homolog 2 (EZH2) expression and downregulation of long non-coding RNA (lncRNA) LET expression are known to be associated with cell apoptosis and proliferation, little is known about the interaction of EZH2 with lncRNA LET. The present study aimed to investigate the interaction of EZH2 and lncRNA LET, and the mechanism of human dermal fibroblast (HDF) proliferation and apoptosis. Tissue samples from 33 burn patients with second- and third-degree burns and 8 controls were collected. mRNA was extracted from the burn tissues for analysis. Isolated primary HDFs were treated with heat or transfected with LET overexpression vectors, and the cell functions and associated proteins in the HDFs were analyzed. Decreased lncRNA LET expression was detected in burn tissues compared with normal skin. Heat-treated HDFs exhibited a reduction in lncRNA LET expression and increase in EZH2 expression. LET gain-of-function experiments in primary HDFs revealed increases in cell proliferation, the proportion of cells in the S stage, and cyclin D1 and cyclin-dependent kinase 4 (CDK4) expression, and reductions in the percentage of apoptotic cells, the Bax/Bcl-2 ratio and caspase-3 expression. RNA immunoprecipitation and chromatin immunoprecipitation assays demonstrated the interaction of ZH2 with lncRNA LET, and of EZH2 with H3K27me3 in HDFs. Furthermore, a negative correlation between lncRNA LET and EZH2 expression was identified. It may be concluded that increased lncRNA-LET expression promoted cell proliferation and inhibited cell apoptosis via the cyclin D1-CDK4 and Bax/Bcl-2/caspase-3 signaling pathways, respectively. Furthermore, the inhibition of lncRNA LET may be regarded as an option for use in the healing of burns.

show abstract

“… 13 In addition, in human dermal fibroblasts, tiron has been shown to inhibit the transcriptional activation of UVB-induced activator protein 1 (AP-1) binding sites on MMP-1 and MMP-3 promoters and was identified as a novel antioxidant for the prevention and treatment of skin photo aging that was induced by UV light exposure. 14 …”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Regulation function of MMP-1 downregulated by siRNA on migration of heat-denatured dermal fibroblasts

Dai

Fan

et al. 2017

Bioengineered

View full text Add to dashboard Cite

Cutaneous wound healing is a complex physiological process that requires the efforts of various cell types and signaling pathways and often results in thickened collagen-enriched healed tissue called a scar. Therefore, the identification of the mechanism of cutaneous wound healing is necessary and has great value in providing better treatment. Here, we demonstrated that MMP-1 inhibition could promote cell proliferation in dermal fibroblasts via the MTT assay. Meanwhile, we investigated cell migration by flow cytometry and tested type I collagenase activity. We found that MMP-1 inhibition promoted cell proliferation and inhibited cell migration and type I collagenase activity. In conclusion, our study demonstrated that MMP-1 might be a potential therapeutic target in cutaneous wound healing.

show abstract

Let-7c miRNA Inhibits the Proliferation and Migration of Heat-Denatured Dermal Fibroblasts Through Down-Regulating HSP70

Cited by 15 publications

References 35 publications

MicroRNA-16 inhibits cell proliferation and migration by targeting heat shock protein 70 in heat-denatured dermal fibroblasts

MicroRNA-16 inhibits cell proliferation and migration by targeting heat shock protein 70 in heat-denatured dermal fibroblasts

EZH2-mediated suppression of lncRNA-LET promotes cell apoptosis and inhibits the proliferation of post-burn skin fibroblasts

RETRACTED ARTICLE: Regulation function of MMP-1 downregulated by siRNA on migration of heat-denatured dermal fibroblasts

Contact Info

Product

Resources

About