Lethal and sub-lethal effects of select macrocyclic lactones insecticides on forager worker honey bees under laboratory experimental conditions

Abdu-Allah, Gamal A. M.; Pittendrigh, Barry R.

doi:10.1007/s10646-017-1872-6

Cited by 13 publications

(6 citation statements)

References 34 publications

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“…Investigations of the acute toxicity and persistence of sublethal effects of insecticides are both needed for the assessment of insecticide efficiency. Sublethal doses of insecticides may affect various the physiological, biochemical, and behavioral traits of exposed insects ( He et al, 2013 ; Abdu-Allah and Pittendrigh, 2018 ). The sublethal effects of insecticides on beneficial arthropods have received considerable attention, while their impacts on pest insects are poorly studied ( Desneux et al, 2007 ; Liu et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Yao

Dong

et al. 2018

Front. Physiol.

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Conopomorpha sinensis Bradley is the dominant borer pest of Litchi chinesis and Euphoria longan. Current management of C. sinensis relies upon insecticide application to adult moths. In addition to the direct mortality induced by insecticides, a sublethal dose of insecticides also affects growth, survival, and reproduction in the exposed insects. Vitellogenin (Vg) and vitellogenin receptor (VgR) are normally identified as essential reproduction-related proteins in insects. In this study, we characterized these two genes from C. sinensis, and investigated their differential responses to sublethal concentrations of insecticide. Cloned CsVg and CsVgR consist of 5391 and 5424-bp open reading frames, which encode proteins of 1796 and 1807 amino acid residues, respectively. The CsVg protein contains the typical vitellogenin, DUF1943 and VWFD domains as other reported lepidopteran Vgs. The CsVgR was characterized as a typical low density lipoprotein receptor with two highly conserved LBD and EGF precursor domains, one hydrophobic transmembrane domain, one cytoplasmic domain, and 13 putative N-glycosylation sites. We next assessed the sublethal effect of four major insecticides on egg-laying in C. sinensis. The toxicity against C. sinensis varied among the insecticides tested, with LC50 values ranging from 0.23 ppm for chlorpyrifos to 20.00 ppm for β-cypermethrin, among which emamectin benzoate (EB) showed a significant negative impact on egg-laying, survival rate, ovarian development, and mating rate of C. sinensis at LC30 doses. Further investigation showed that the transcriptional level of CsVg and CsVgR were impaired in different way at 24, 48, and 72 h after EB exposure, and this result was in agreement with the diminished egg-laying of C. sinensis in the sublethal concentration EB-treated group. A repressed transcription level of CsVgR was observed at 48 h after treatment, suggesting that EB elicits a delayed response in the abundance of CsVgR. These results established different roles of CsVg and CsVgR in response to the sublethal effect of insecticides. CsVg might be a better parameter than CsVgR for assessing the effect of sublethal insecticides on reproduction in C. sinensis.

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Section: Introductionmentioning

confidence: 99%

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Yao

Dong

et al. 2018

Front. Physiol.

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“…Results showed that bees could be contaminated by insecticides containing active ingredients such as cypermethrin, deltamethrin and emamectin. Additionally, these active ingredients had lethal and sublethal effects on forager worker honeybees in experimental conditions [37].…”

Section: Discussionmentioning

confidence: 96%

Farmer knowledge and perceptions of agrochemicals use on honeybee community and honey production in Benin

Dassou

Anagonou

Vohouhe

et al. 2020

Preprint

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Background Apiculture faces many constraints including the use of agrochemicals. Chronic exposure to chemical pesticide use results in the loss of honeybee colonies and alters detoxification mechanisms and immune responses, driving them more susceptible to parasites. This study aims at investigating the impact of agrochemical use on honeybee community and honey production in northern Benin. Methods For this purpose, 100 beekeepers were surveyed using a survey questionnaire. Information on treated crops, the distance of beehives from these crops, the effect of insecticides and herbicides, adaptation patterns and honey production were collected through individual interviews. Socio-demographic data and farmer’s knowledge on agrochemical uses and their impacts on honeybee communities were determined. Information were also collected on alternative methods to agrochemicals, beehive types and honey taste. Results Apicultural investigations have shown that chemical pesticides were applied in fields of crops and that beehives were mostly located near treated fields. The most used chemical pesticides were Kalach (glyphosate), Callifor G (glyphosate, fluometuron, prometyn), Atrazila 80 wp (atrazin), Herbextra (glyphosate) and Cotonix (deltamethrin and chlorpyriphos). The majority of beekeepers (79%) recognize the effect of pesticides and use alternatives to pesticides to reduce the risk. In general, 49% of beekeepers found the decrease in the big breed of bees and 40% that of the small breed. The honey production was higher when the beehives were installed far from the treated fields. Conclusion and implications: This study allowed to knowing the direct and indirect effects of the agrochemical use on beekeeping. Alternative pest management methods to the pesticide use practiced by farmers would lead to sustainable improvements of honey production in Benin.

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“…Considering the greatly controlled circumstances and precise laboratorial design, the LC 50 values from acute toxicity determinations become worthy if they can be adopted to forecast the influences of compounds on the pollinators under field environments 21 , 28 . The procured LC 50 values of pesticides in our present research were almost unlikely in the ecosystem, and such values can only be happened in a specific case, such as direct usage or unlucky leakage 23 , 24 .…”

Section: Discussionmentioning

confidence: 99%

“…The toxicity of single compounds to pollination insects has been carried out by most of the previous studies 21 . However, pesticides are rarely found as individual chemicals in the environments 22 .…”

Section: Introductionmentioning

confidence: 99%

Mixture effects of thiamethoxam and seven pesticides with different modes of action on honey bees (Aplis mellifera)

Wang

et al. 2023

Sci Rep

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Even though honey bees in the field are routinely exposed to a complex mixture of many different agrochemicals, few studies have surveyed toxic effects of pesticide mixtures on bees. To elucidate the interactive actions of pesticides on crop pollinators, we determined the individual and joint toxicities of thiamethoxam (THI) and other seven pesticides [dimethoate (DIM), methomyl (MET), zeta-cypermethrin (ZCY), cyfluthrin (CYF), permethrin (PER), esfenvalerate (ESF) and tetraconazole (TET)] to honey bees (Aplis mellifera) with feeding toxicity test. Results from the 7-days toxicity test implied that THI elicited the highest toxicity with a LC50 data of 0.25 (0.20–0.29) μg mL−1, followed by MET and DIM with LC50 data of 4.19 (3.58–4.88) and 5.30 (4.65–6.03) μg mL−1, respectively. By comparison, pyrethroids and TET possessed relatively low toxicities with their LC50 data from the range of 33.78 (29.12–38.39) to 1125 (922.4–1,442) μg mL−1. Among 98 evaluated THI-containing binary to octonary mixtures, 29.59% of combinations exhibited synergistic effects. In contrast, 18.37% of combinations exhibited antagonistic effects on A. mellifera. Moreover, 54.8% pesticide combinations incorporating THI and TET displayed synergistic toxicities to the insects. Our findings emphasized that the coexistence of several pesticides might induce enhanced toxicity to honey bees. Overall, our results afforded worthful toxicological information on the combined actions of neonicotinoids and current-use pesticides on honey bees, which could accelerate farther comprehend on the possible detriments of other pesticide mixtures in agro-environment.

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Lethal and sub-lethal effects of select macrocyclic lactones insecticides on forager worker honey bees under laboratory experimental conditions

Cited by 13 publications

References 34 publications

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Characterization of Vitellogenin and Vitellogenin Receptor of Conopomorpha sinensis Bradley and Their Responses to Sublethal Concentrations of Insecticide

Farmer knowledge and perceptions of agrochemicals use on honeybee community and honey production in Benin

Mixture effects of thiamethoxam and seven pesticides with different modes of action on honey bees (Aplis mellifera)

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