Letter by Ni et al Regarding Article “Spotty Calcium on Cervicocerebral Computed Tomography Angiography Associates with Increased Risk of Ischemic Stroke”

Ni, Xiaoxing; Cao, Shugang; Xu, Wenli

doi:10.1161/strokeaha.119.025913

Cited by 1 publication

(1 citation statement)

References 6 publications

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“…In China, the majority of deaths caused by brain complications are caused by ischemic stroke. At the same time, approximately 30% of these patients permanently lose the ability to work, which has a negative impact on the life of patients (Ni et al 2019;Wang et al 2019). Many studies have found that the expression of miRNAs changes after ischemic stroke and is involved in the regulation of the progression of ischemic stroke (Jiang et al 2019;Li Z et al 2021;Liu et al 2022).…”

Section: Discussionmentioning

confidence: 99%

miR-497-5p promoted neuronal injury in ischemic stroke by inhibiting the BDNF/TrkB/PI3K/Akt pathway

Gong,

He,

et al. 2024

gpb

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The aim of this study was to investigate the molecular mechanism by which miR-497-5p regulates neuronal injury after ischemic stroke through the BDNF/TrkB/Akt signaling pathway. PC12 cells were used to construct a stroke injury model by oxygen-glucose deprivation/reoxygenation (OGD/R). The expression level of miR-497-5p was measured by RT-qPCR. CCK-8 kit was used to detect cell viability. Cell apoptosis and reactive oxygen species (ROS) were detected by flow cytometry. MDA and SOD detection kits were used to detect MDA content and SOD activity. A double luciferase reporter system was used to verify the targeting relationship between miR-497-5p and BDNF. The expression of BDNF, TrkB, p-TrkB, Akt and p-Akt was detected by Western blot. We have found that miR-497-5p expression was inhibited after treatment with OGD/R. Simultaneously, cell apoptosis, MDA content and ROS were upregulated, while cell viability and SOD were significantly decreased in PC12 cells. The effects of OGD/R on PC12 cells were reversed with the downregulation of miR-497-5p. A double luciferase reporter assay demonstrated that miR-497-5p negatively targets BDNF. BDNF inhibited cell apoptosis and oxidative stress injury in PC12 cells. These findings suggest that miR-497-5p aggravates neuronal injury in experimental model of ischemic stroke by inhibiting the BDNF/TrkB/PI3K/Akt signaling pathway.

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