Letter to editor Listy do redakcji

Pawlus, Bartłomiej; Markowicz-Pawlus, Ewa; Špidlen, Miloš

doi:10.5114/kitp.2013.34316

Cited by 2 publications

(4 citation statements)

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“…32 P300 regulates the acetylation status of lysine residues of histones at HIF-1 target genes, relaxing the chromatin structure and promoting HIF-1-dependent gene transcription. 23,34,35 A previous study revealed that SIRT6 suppressed HIF1α transcriptional activity by directly deacetylating H3K9 at HIF1α target gene promoters and increased protein synthesis and stability of HIF1α were observed in SIRT6deficient cells. 25,36 The above results suggested that SIRT6 could downregulate the transcription of HIF1α target genes by influencing HIF1α transcriptional activity and protein level.…”

Section: Discussionmentioning

confidence: 99%

“…The histone acetyltransferase p300 is responsible for histone acetylation, facilitating nucleosome rearrangement and DNA binding by RNA-polymerase II and transcription factors. 23 Under OGD/R conditions, H3K9 acetylation (H3K9Ac) at the Ero1α promoter was also significantly increased (Figure 4A). Based on these findings, the effects of p300 modulation of H3K9Ac and HIF1α at the Ero1α promoter were further examined, and the results showed that CBP30 (a selective inhibitor for inhibiting p300 activity 24 ) significantly decreased the accumulation of H3K9Ac and HIF1α at the Ero1α promoter (Figure 4C).…”

Section: Sirt6 Inhibits Ero1α By Suppressing Hif1α Accumulation and H...mentioning

confidence: 96%

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SIRT6 deficiency in endothelial cells exacerbates oxidative stress by enhancing HIF1α accumulation and H3K9 acetylation at the Ero1α promoter

Guo,

Yu,

Zhao

et al. 2023

Clinical & Translational Med

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BackgroundSIRT6, an important NAD+‐dependent protein, protects endothelial cells from inflammatory and oxidative stress injuries. However, the role of SIRT6 in cardiac microvascular endothelial cells (CMECs) under ischemia‒reperfusion injury (IRI) remains unclear.MethodsThe HUVECs model of oxygen–glucose deprivation/reperfusion (OGD/R) was established to simulate the endothelial IRI in vitro. Endoplasmic reticulum oxidase 1 alpha (Ero1α) mRNA and protein levels in SIRT6‐overexpressing or SIRT6‐knockdown cells were measured by qPCR and Western blotting. The levels of H2O2 and mitochondrial reactive oxygen species (ROS) were detected to evaluate the status of oxidative stress. The effects of SIRT6 deficiency and Ero1α knockdown on cellular endoplasmic reticulum stress (ERS), inflammation, apoptosis and barrier function were detected by a series of molecular biological experiments and functional experiments in vitro. Chromatin immunoprecipitation, Western blotting, qPCR, and site‐specific mutation experiments were used to examine the underlying molecular mechanisms. Furthermore, endothelial cell‐specific Sirt6 knockout (ecSirt6−/−) mice were subjected to cardiac ischemia‒reperfusion surgery to investigate the effects of SIRT6 in CMECs in vivo.ResultsThe expression of Ero1α was significantly upregulated in SIRT6‐knockdown endothelial cells, and high Ero1α expression correlated with the accumulation of H2O2 and mitochondrial ROS. In addition, SIRT6 deficiency increased ERS, inflammation, apoptosis and endothelial permeability, and these effects could be significantly attenuated by Ero1α knockdown. The deacetylase catalytic activity of SIRT6 was important in regulating Ero1α expression and these biological processes. Mechanistically, SIRT6 inhibited the enrichment of HIF1α and p300 at the Ero1α promoter through deacetylating H3K9, thereby antagonizing HIF1α/p300‐mediated Ero1α expression. Compared with SIRT6‐wild‐type (SIRT6‐WT) cells, cells expressing the SIRT6‐H133Y‐mutant and SIRT6‐R65A‐mutant exhibited increased Ero1α expression. Furthermore, ecSirt6−/− mice subjected to ischemia‒reperfusion surgery exhibited increased Ero1α expression and ERS in CMECs and worsened injuries to microvascular barrier function and cardiac function.ConclusionsOur results revealed an epigenetic mechanism associated with SIRT6 and Ero1α expression and highlighted the therapeutic potential of targeting the SIRT6‐HIF1α/p300‐Ero1α axis.

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Section: Discussionmentioning

confidence: 99%

Section: Sirt6 Inhibits Ero1α By Suppressing Hif1α Accumulation and H...mentioning

confidence: 96%

SIRT6 deficiency in endothelial cells exacerbates oxidative stress by enhancing HIF1α accumulation and H3K9 acetylation at the Ero1α promoter

Guo,

Yu,

Zhao

et al. 2023

Clinical & Translational Med

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“…The authors of papers [5,6,12] analysed variations of areal (3D) parameters. Authors of works [7,12] studied variability of areal surface topography parameters. The main aim of this work was to study the effect of light intensity variation on change of surface topography parameters.…”

Section: Introductionmentioning

confidence: 99%

“…Variability of 2D profile parameters on typical manufactured surfaces was studied [15]. The authors of papers [5,6,12] analysed variations of areal (3D) parameters. Authors of works [7,12] studied variability of areal surface topography parameters.…”

Section: Introductionmentioning

confidence: 99%

Importance of light intensity selection in the surface topography measurements using optical profilometer

Dzierwa

2015

Mechanik

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Several isotropic and anisotropic surface topographies were analysed. Surface measurements were made using white light interferometer Talysurf CCI Lite, version 2.8.2.95. All surfaces were measured with different light intensity. Light intensity was changed in the range 10%-90% every time at 5%. After measurement each surface was levelled and nonmeasured points were filled up. Parameters from ISO 25178 group and parameters from the Sk family were calculated. Parameters were calculated using the software Talymap Gold, version 6.0. Influence of light intensity on the change of surface topography parameters was analysed.

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Letter to editor Listy do redakcji

Cited by 2 publications

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SIRT6 deficiency in endothelial cells exacerbates oxidative stress by enhancing HIF1α accumulation and H3K9 acetylation at the Ero1α promoter

SIRT6 deficiency in endothelial cells exacerbates oxidative stress by enhancing HIF1α accumulation and H3K9 acetylation at the Ero1α promoter

Importance of light intensity selection in the surface topography measurements using optical profilometer

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