Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice

Brunetta, Henver Simionato; Paula, Gabriela Cristina de; Fritzen, Matheus; Cecchini, Manuela Sozo; Santos, Gustavo Jorge dos; Nazari, Evelise Maria; Rafacho, Alex; de, Andreza Fabro; Nunes, Everson Araújo

doi:10.1016/j.jff.2019.103544

Cited by 8 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, skeletal muscle FAO appears to be associated with mitochondrial biogenesis [82] and expression of certain genes, including those for PGC-1α and SIRT1 that regulate energy metabolism via modulation of thermogenesis, mitochondrial number, and FAO [80]. Leu modulates energy metabolism, in part, through the regulation of mitochondrial biogenesis, and promotes FAO and mitochondrial biogenesis [83]. Leu promotes partitioning energy from the adipocytes to the muscle cells, leading to decreased lipid storage in the adipocytes and increased fat utilization in the muscles [84].…”

Section: Leu and Lipid Metabolism In Skeletal Musclementioning

confidence: 99%

Leucine Supplementation: A Novel Strategy for Modulating Lipid Metabolism and Energy Homeostasis

Zhang

Guo

et al. 2020

Nutrients

View full text Add to dashboard Cite

Lipid metabolism is an important and complex biochemical process involved in the storage of energy and maintenance of normal biological functions. Leucine, a branched amino acid, has anti-obesity effects on glucose tolerance, lipid metabolism, and insulin sensitivity. Leucine also modulates mitochondrial dysfunction, representing a new strategy to target aging, neurodegenerative disease, obesity, diabetes, and cardiovascular disease. Although various studies have been carried out, much uncertainty still exists and further studies are required to fully elucidate the relationship between leucine and lipid metabolism. This review offers an up-to-date report on leucine, as key roles in both lipid metabolism and energy homeostasis in vivo and in vitro by acceleration of fatty acid oxidation, lipolysis, activation of the adenosine 5′-monophosphate-activated protein kinase (AMPK)–silent information regulator of transcription 1 (SIRT1)–proliferator-activated receptor γ coactivator-1α (PGC-1α) pathway, synthesis, and/or secretion of adipokines and stability of the gut microbiota.

show abstract

Section: Leu and Lipid Metabolism In Skeletal Musclementioning

confidence: 99%

Leucine Supplementation: A Novel Strategy for Modulating Lipid Metabolism and Energy Homeostasis

Zhang

Guo

et al. 2020

Nutrients

View full text Add to dashboard Cite

show abstract

“…This may be due to differences in mTORC1 activity in muscle at time of collection; traditionally, tissue is collected and measured following fasting which reduces mTORC1 signaling while feeding and insulin stimulate mTORC1 thereby increasing mitochondrial respiration [127]. In support, ex vivo pretreatment of muscle with leucine, an mTORC1 stimulator, increases mitochondrial respiration which is then attenuated by adding rapamycin [128].…”

Section: Exercise + Rapamycinmentioning

confidence: 99%

Geroprotector drugs and exercise: friends or foes on healthy longevity?

Elliehausen,

Anderson,

Diffee

et al. 2023

BMC Biol

View full text Add to dashboard Cite

Physical activity and several pharmacological approaches individually combat age-associated conditions and extend healthy longevity in model systems. It is tantalizing to extrapolate that combining geroprotector drugs with exercise could extend healthy longevity beyond any individual treatment. However, the current dogma suggests that taking leading geroprotector drugs on the same day as exercise may limit several health benefits. Here, we review leading candidate geroprotector drugs and their interactions with exercise and highlight salient gaps in knowledge that need to be addressed to identify if geroprotector drugs can have a harmonious relationship with exercise.

show abstract

“…Swiss mice were chosen as a model of HFD-induced obesity based on previous studies from our group [46,47]. In these previous studies, Swiss mice strand was shown to respond efficiently to the HFD, developing obesity features (e.g., elevated body mass and adiposity, glucose intolerance and insulin resistance) [46,47]. Thus, mice were acclimatized for 4 weeks before being randomly assigned to one of the four experimental groups (n = 10-15 per group).…”

Section: Experimental Design and Groupsmentioning

confidence: 99%

Pep19 Has a Positive Effect on Insulin Sensitivity and Ameliorates Both Hepatic and Adipose Tissue Phenotype of Diet-Induced Obese Mice

Silvério

Barth

Heimann³

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Peptide DIIADDEPLT (Pep19) has been previously suggested to improve metabolic parameters, without adverse central nervous system effects, in a murine model of diet-induced obesity. Here, we aimed to further evaluate whether Pep19 oral administration has anti-obesogenic effects, in a well-established high-fat diet-induced obesity model. Male Swiss mice, fed either a standard diet (SD) or high-fat diet (HFD), were orally administrated for 30 consecutive days, once a day, with saline vehicle or Pep19 (1 mg/kg). Next, several metabolic, morphological, and behavioral parameters were evaluated. Oral administration of Pep19 attenuated HFD body-weight gain, reduced in approximately 40% the absolute mass of the endocrine pancreas, and improved the relationship between circulating insulin and peripheral insulin sensitivity. Pep19 treatment of HFD-fed mice attenuated liver inflammation, hepatic fat distribution and accumulation, and lowered plasma alanine aminotransferase activity. The inguinal fat depot from the SD group treated with Pep19 showed multilocular brown-fat-like cells and increased mRNA expression of uncoupling protein 1 (UCP1), suggesting browning on inguinal white adipose cells. Morphological analysis of brown adipose tissue (BAT) from HFD mice showed the presence of larger white-like unilocular cells, compared to BAT from SD, Pep19-treated SD or HFD mice. Pep19 treatment produced no alterations in mice behavior. Oral administration of Pep19 ameliorates some metabolic traits altered by diet-induced obesity in a Swiss mice model.

show abstract

Leucine increases muscle mitochondrial respiration and attenuates glucose intolerance in diet-induced obesity in Swiss mice

Cited by 8 publications

References 46 publications

Leucine Supplementation: A Novel Strategy for Modulating Lipid Metabolism and Energy Homeostasis

Leucine Supplementation: A Novel Strategy for Modulating Lipid Metabolism and Energy Homeostasis

Geroprotector drugs and exercise: friends or foes on healthy longevity?

Pep19 Has a Positive Effect on Insulin Sensitivity and Ameliorates Both Hepatic and Adipose Tissue Phenotype of Diet-Induced Obese Mice

Contact Info

Product

Resources

About