Leucodepletion leads to component‐like storage stability of whole blood – suggesting its homologous use?

Picker, Susanne M.; Stürner, S S; Oustianskaja, L; Gathof, Birgit

doi:10.1111/j.1423-0410.2004.00570.x

Cited by 16 publications

(20 citation statements)

References 14 publications

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“…In vitro quality was analyzed for total and supernatant Hb, Hct, rate of hemolysis, potassium, intracellular ATP, glucose, and lactate levels as well as for pH value and blood gases (at 22°C) and determined with standard procedures as described previously. 12 …”

Section: In Vitro Measurementsmentioning

confidence: 97%

In vitro quality of red blood cells (RBCs) collected by multicomponent apheresis compared to manually collected RBCs during 49 days of storage

2007

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Section: In Vitro Measurementsmentioning

confidence: 97%

In vitro quality of red blood cells (RBCs) collected by multicomponent apheresis compared to manually collected RBCs during 49 days of storage

2007

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“…However, the results of this study are still valuable for autologous transfusion and the many clinical settings, particularly in developing countries [35] where allogeneic whole blood is still mainly employed. Furthermore, the use of whole blood is gaining renewed interest in developed countries [36][37][38] because it has been shown that leukocyte depletion results in a considerably improved plasma quality in whole blood [39]. cells lose their deformability during storage has been consistently shown by indirect methods using filtration techniques [20] or the LORCA system [21].…”

Section: Discussionmentioning

confidence: 99%

Deformability of Red Blood Cells and Correlation with ATP Content during Storage as Leukocyte-Depleted Whole Blood

Karger

Lukow

Kretschmer³

2012

Transfus Med Hemother

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KeywordsATP content · Deformability · Quality · Red cell storage · Storage lesion · In vivo survival · Whole blood Summary Background: Storage duration of red cells has been associated with increased morbidity and mortality following transfusion. This association has been attributed to the loss of deformability of stored red cells leading to deterioration of microvascular perfusion. ATP content is considered a critical determinant of the deformability of stored red cells. Methods: ATP content and deformability were determined after storage for up to 49 days in 40 leukocyte-depleted whole blood units. Red cell deformability was determined using a laser-assisted optical rotational cell analyzer (LORCA ® ) employing shear stress (SS) ranging from 0.3 to 30 Pa. Deformability was expressed as the elongation index (EI). EI was correlated with ATP content. Results: ATP content decreased from 3.5 to 1.7 mol/g hemoglobin. EI increased from 0.03 to 0.05 at an SS of 0.

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“…Hence, the lower RBC loss noted with double-unit filtration could be considered as an advantage over single-unit filtration during 2-RBCA. The RBC loss of some TA and MCS+ units exceeded 15% which is the upper FDA (US Food and Drug Administration) limit [3,12]. In addition, a mean of 153 ml of absolute RBC mass, which is the acceptable mean RBC content for leukoreduced apheresis RBCs established by the AABB standards [13], was not reached by TA and MCS+ units.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro measurements included hematocrit, total and supernatant (free) Hb as well as ATP levels, supernatant potassium, glucose, lactate and pH. These measurements were performed by standard procedures [12]. Sterility was verified by microbial culture testing of each RBC unit derived from PLT-RBC collections and of an arbitrarily selected unit from each 2-RBCA.…”

Section: In Vitro Measurementsmentioning

confidence: 99%

Comparison of in vitro Characteristics of Leukodepleted Red Blood Cells (RBCs) Derived from Apheresis: Impact of Filter Performance

Radojska¹,

Picker²,

Gathof³

2006

Transfus Med Hemother

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Novel apheresis systems allow collection of packed red blood cells (RBCs) either by double RBC apheresis (2-RBCA) or along with platelet (PLT) concentrates (RBCPLT). The objective of this study was to evaluate filtration performance in connection with in vitro storage quality of filtered RBCs derived from the devices Baxter Amicus (AM), Baxter Alyx (AX), Haemonetics MCS Plus (MCS+) and Gambro Trima Accel (TA). Materials and Methods: 66 2-RBCA (15 AX, 10 MCS+, 8 TA) and 33 RBC-PLT collections (15 AM, 9 MCS+, 9 TA) were analyzed. RBC filtration was performed at room temperature within 2-4 h of collection using white blood cell (WBC) reduction filters either integrated in the disposable sets or provided by a filtration device. In vitro parameters were measured after filtration and after 42 days of storage. Results: Except for 1 paired AX unit, all RBCs contained residual WBCs of < 1 × 106/unit. TA provided the best log reduction in WBCs and PLTs but demonstrated the longest filtration times and smallest RBC recoveries. AX and MCS+ had significantly shorter filtration times than AM, however, AM obtained the best RBC recoveries. After filtration, all packed RBCs showed similar in vitro characteristics. At the end of storage, lower values for hemolysis (TA) and ATP preservation (AX) were related to the post-filtration WBC amount, with TA demonstrating the lowest and AX the highest values. Conclusion: While differences in RBC loss were noted, satisfactory filter performance in terms of WBC and PLT removal was observed with most RBC units. In AX procedures, leukodepletion could be improved.

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Leucodepletion leads to component‐like storage stability of whole blood – suggesting its homologous use?

Abstract: Our data indicate that the quality of LWB is comparable to that of components during 42 days of storage. Thus, LWB could be an interesting option for using to facilitate and economize the blood supply, especially for surgical or trauma patients.

Cited by 16 publications

References 14 publications

In vitro quality of red blood cells (RBCs) collected by multicomponent apheresis compared to manually collected RBCs during 49 days of storage

In vitro quality of red blood cells (RBCs) collected by multicomponent apheresis compared to manually collected RBCs during 49 days of storage

Deformability of Red Blood Cells and Correlation with ATP Content during Storage as Leukocyte-Depleted Whole Blood

Comparison of in vitro Characteristics of Leukodepleted Red Blood Cells (RBCs) Derived from Apheresis: Impact of Filter Performance

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