Leukocytoclasis: ultrastructural in situ nick end labeling study in anaphylactoid purpura

Yamamoto, Takashi; Kaburagi, Yutaka; Izaki, Seiichi; Tanaka, Toshimitsu; Kitamura, Keijiro

doi:10.1016/s0923-1811(00)00092-x

Cited by 11 publications

(12 citation statements)

References 29 publications

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“…Recent studies on the nature of the neutrophilic infiltrate showed a strong positive staining with in situ nick end labeling techniques, thereby demonstrating DNA breakdown in the infiltrating neutrophils. In these lesions, only a minor part is typically apoptotic, whereas the majority of neutrophils shows breakdown of DNA with disintegrated cytoplasmic organelles and plasma membrane 42,43. The phenomenon of leukocytoclasia is interesting since normally dying cells are quickly engulfed by phagocytes, particularly at sites of inflammation.…”

Section: Disturbances In the Clearance Of Neutrophilsmentioning

confidence: 99%

Activation, Apoptosis, and Clearance of Neutrophils in Wegener's Granulomatosis

Rossum

Limburg

Kallenberg

2005

Annals of the New York Academy of Sciences

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Wegener's granulomatosis (WG) is strongly associated with the presence of antineutrophil cytoplasmic autoantibodies (ANCAs). Within WG these ANCAs are usually (80-90%) directed against the azurophilic enzyme proteinase 3, the so called PR3-ANCA. A pathophysiological role for these autoantibodies, supported by numerous in vitro and in vivo studies, is specifically based on their capacity to bind and activate neutrophils and potentially may damage vessels. In this review, the pathogenic potential of different developmental stages of the neutrophil in the pathogenesis of WG is discussed. After release from the bone marrow into the circulation, neutrophils can be primed by TNFalpha and become attached to locally activated endothelium. Once attached to the endothelium, ANCAs can fully activate these primed neutrophils. In this activation process, the degree of activation after stimulation with PR3-ANCAs associates with the level of PR3 expression on the membrane of the neutrophil. Following activation, infiltrated neutrophils become apoptotic with further membrane expression of PR3. In WG patients, clearance of apoptotic neutrophils can be disturbed due to the opsonization of PR3-expressing apoptotic neutrophils with PR3-ANCAs, thereby perpetuating inflammation by the release of proinflammatory cytokines during clearance; or it may favor autoimmunity by PR3 presentation in an inflammatory environment. Furthermore, the presence of ANCAs and the release of the vessel-related pentraxin PTX3 may lead to the persistence of late apoptotic neutrophils in tissues, thereby inducing leukocytoclastic lesions that are characteristic in patients with WG. All together, alive neutrophils as well as apoptotic neutrophils play a key role in different inflammatory phenomena seen in patients suffering from WG.

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Section: Disturbances In the Clearance Of Neutrophilsmentioning

confidence: 99%

Activation, Apoptosis, and Clearance of Neutrophils in Wegener's Granulomatosis

Rossum

Limburg

Kallenberg

2005

Annals of the New York Academy of Sciences

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“…E/M studies of the leukocytoclastic lesions, have suggested that there may be a defect in the clearance of apoptotic neutrophils. The minority of neutrophils in this study showed typical apoptotic changes of the condensed and marginated nuclei, while the majority showed intact nuclei with disintegrated cytoplasmic organelles and plasma membranes (Yamamoto, 2000). We have demonstrated significantly higher rate of survival and lower rate of apoptosis in AASV neutrophils as compared to neutrophils from healthy blood donors (HBDs) (Un-published data).…”

Section: Neutrophil Apoptosis and Aasvmentioning

confidence: 55%

The Role of Proteinase 3 and Neutrophils in ANCA-Associated Systemic Vasculitis

AbdGawad¹

2011

Advances in the Etiology, Pathogenesis and Pathology of Vasculitis

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“…E/M studies of the leukocytoclastic lesions, in patients with leukocytoclastic vasculitis, have suggested that there may be a defect in the clearance of apoptotic neutrophils. The minority of neutrophils in this study showed typical apoptotic changes of the condensed and marginated nuclei, while the majority showed intact nuclei with disintegrated cytoplasmic organelles and plasma membranes [95]. Apoptotic neutrophils may, in fact, be a source of immunologically exposed neutrophil antigens that promote the production of ANCAs.…”

Section: Role Of Neutrophil Apoptosis In Aasvmentioning

confidence: 66%

History, Classification and Pathophysiology of Small Vessel Vasculitis

AbdGawad¹

2013

Updates in the Diagnosis and Treatment of Vasculitis

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Leukocytoclasis: ultrastructural in situ nick end labeling study in anaphylactoid purpura

Cited by 11 publications

References 29 publications

Activation, Apoptosis, and Clearance of Neutrophils in Wegener's Granulomatosis

Activation, Apoptosis, and Clearance of Neutrophils in Wegener's Granulomatosis

The Role of Proteinase 3 and Neutrophils in ANCA-Associated Systemic Vasculitis

History, Classification and Pathophysiology of Small Vessel Vasculitis

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