Leukosialin (CD43) behavior during adhesion of human monocytic THP-1 cells to red blood cells

Solèr, M.; Mérant, Catherine; Servant, C; Fraterno, M.; Allasia, Claude; Lissitzky, Jean‐Claude; Bongrand, Pierre; C, Foa

doi:10.1002/jlb.61.5.609

Cited by 28 publications

(25 citation statements)

References 49 publications

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“…Since the 7 patients studied with a drastic decrease in the expression of CD43 sialylo-dependent epitope did not exhibit a decrease of MFI with the L10 mAb, we suggest that the modification was more a dys-sialylation rather than a diminished number of CD43 molecules. Similar results were obtained in a previous work on neuraminidase-treated cells [9]. In some cases, we observed two subpopulations of monocytes with differential expression of CD43 and such features were observed in the rat [15, 16].…”

Section: Discussionsupporting

confidence: 80%

“…Adhesive and antiadhesive properties have been attributed to CD43 [5, 6]. CD43 has been demonstrated to be redistributed during adhesion, both on lymphocytes [7, 8]and on monocytes [9]. Moreover, we have demonstrated that on cultured human monocytes exposed to IFN-γ and TNF-α stimulation, CD43 is partially dys-sialylated [9].…”

Section: Introductionmentioning

confidence: 94%

“…CD43 has been demonstrated to be redistributed during adhesion, both on lymphocytes [7, 8]and on monocytes [9]. Moreover, we have demonstrated that on cultured human monocytes exposed to IFN-γ and TNF-α stimulation, CD43 is partially dys-sialylated [9]. To investigate CD43 behavior in vivo, we chose to investigate CD43 expression on peripheral blood mononuclear cells (PBMC) from kidney transplant recipient patients whose treatment (CsA, prednisone, and azathioprine) targets monocytes and lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

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Comparative CD43 Behavior on Monocytes and Lymphocytes in Kidney Transplants

Jego

Sabri²,

Solèr³

et al. 2000

Nephron

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In human cultured monocytic cells stimulated by cytokines, CD43 was demonstrated to exhibit a modification of sialylated epitopes (dys-sialylation) [Soler et al: Leukoc Biol 1997;61:609–618]. Therefore, we chose to investigate CD43 behavior on patients who present pathological status implicating monocytes after renal graft (KTR). We performed flow cytometry after immune staining using monoclonal antibodies to CD43 sialic acid-dependent (L60) and -independent (L10) epitopes. Compared to normal controls, mean fluorescence intensity was never altered on lymphocytes. Conversely, on monocytes, we found different profiles with L60: 26% of patients having normal CD43 expression, 54% displayed decreased values and 20% had a double population of monocytes, the major one being normal and the minor one with a very low staining. Decreased values were more frequent among KTR during the first 3 months following transplantation. L10 immunostaining was not altered on monocytes in patients with low values of CD43 staining by L60, confirming that the mechanism involved was a CD43 dys-sialylation. We investigated a possible role of cyclosporin (CsA) on human monocytic (THP-1) and lymphoid (Jurkat) cell lines. CsA decreases CD43 expression in monocytic and not in lymphoid cell lines and could be responsible for the specific dys-sialylation of KTR monocytes. Whatever, CD43 dys-sialylation might lead to functional abnormalities of monocytes in KTR, possibly involving the adhesion process.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative CD43 Behavior on Monocytes and Lymphocytes in Kidney Transplants

Jego

Sabri²,

Solèr³

et al. 2000

Nephron

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“…Changes in the glycosylation pattern of its extracellular domain allow CD43 to function as a proadhesive counter receptor for galectin-1, ICAM-1, E-selectin, sialoadhesin and MHC Class I molecules. 16,23,[25][26][27][28]37,39,40 While CD43 is normally restricted in its expression to leukocytes and platelets, aberrant expression has been consistently described in colon and salivary gland cancers. [41][42][43][44] In addition, limited analysis suggested that NSCLC but not SCLC might also be characterized by CD43 expression.…”

Section: Discussionmentioning

confidence: 99%

CD43 in the nucleus and cytoplasm of lung cancer is a potential therapeutic target

Cash

Andersen

et al. 2012

Intl Journal of Cancer

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CD43 is a transmembrane sialoglycoprotein. Normally the molecule is only produced by white blood cells where it regulates functions such as intercellular adhesion, intracellular signaling, apoptosis, migration and proliferation. Two CD43 antibodies were used to interrogate 66 cases of non-small cell lung cancer (NSCLC) and 24 cases of small cell lung cancer (SCLC). In addition, we engineered the CD43-positive lung cancer cell line A549 to stably express either non-targeted or CD43-targeted small-interfering RNA (siRNA). These lines were then subjected to in vitro assays of apoptosis, natural killer (NK) cell cytotoxicity, intercellular adhesion and transendothelial migration. A xenograft mouse model evaluated the ability of the lines to grow primary tumors in vivo. CD43 was found to be expressed in the majority of both SCLC and NSCLC. Inclusive of CD43-negative tumors, differential patterns of nuclear and cytoplasmic expression of CD43 define four molecular subcategories of lung cancer. Targeting CD43 in A549 lung cancer cells, increased homotypic adhesion, decreased heterotypic adhesion and transendothelial migration, increased susceptibility to apoptosis and increased vulnerability to lysis by NK cells. Furthermore, targeting inhibited the growth of primary tumors in nude mice.Despite major advances in the field of medical oncology, lung cancer remains the leading cause of cancer death in the United States among both men and women. With an estimated 221,130 new cases and 156,940 deaths in 2011, lung cancer is responsible for more than 25% of all cancer deaths. 1 Two studies have reported that lung cancer might be characterized by expression of the sialoglycoprotein CD43 normally only produced by leukocytes. 2,3 The first study reported that 13 out of 13 cases of non-small cell lung cancer (NSCLC) expressed CD43 while two out of two cases of small cell lung cancer (SCLC) were CD43-negative. 2 The second study reported that one out of three primary lung tumors exhibited CD43 expression. 3 One of these three tumors was a smallcell carcinoma and the other two were squamous cell carcinomas. Here, we report the analysis of 90 cases of lung cancer using antibodies that recognize either the N or C terminus of CD43. In addition, we report the functional consequences of expressing CD43-targeted small-interfering RNA (siRNA) in the lung cancer cell line A549. Material and Methods Patient materialParaffin-embedded formalin-fixed tissues representing 90 cases of lung cancer and one mild case of lung silicosis were provided free-of-charge by the Gundersen Foundation BioBank (http://www.gundluth.org/biobank). Each case was sectioned, stained with hematoxylin and eosin and the histological diagnosis verified. All experimental procedures were

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“…12,41,43 Exclusions of CD43 from the leading edge of polarized cells and from attachment sites further suggest possible function of CD43 in cell rounding. [11][12][13][14] Meanwhile, several adhesion molecules are localized, if not enriched, in leukocyte microvilli. 22,24,25,29 We also observed localization of α4β1 integrin in microvilli in CD43-HEK293T cells.…”

Section: Methodsmentioning

confidence: 99%