Levels and complexity of IgA antibody against oral bacteria in samples of human colostrum

Petrechen, L.N.; Zago, F.H.; Sesso, Maria Lucia Talarico; Bertoldo, Barbara Bellocchio; Silva, C.B.; Azevedo, K.P.; Pereira, Sanívia Aparecida de Lima; Geraldo-Martins, Vinícius Rangel; Ferriani, Virgínia Paes Leme; Nogueira, Ruchele Dias

doi:10.1016/j.imbio.2014.08.009

Cited by 13 publications

(21 citation statements)

References 36 publications

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“…By searching available published peer‐reviewed studies on breast milk, we found that most of the studies were conducted on the colostrum (Koenig, Diniz, Barbosa, & Vaz, ; Petrechen, et al, ). Some of these studies were conducted on the mature milk in the first month of lactation, focusing on IgA in breast milk (Petrechen, et al, 2014) because it is the most abundant Ig in breast milk (Palmeira et al, ).…”

Section: Introductionmentioning

confidence: 99%

Alterations of immunoglobulin G and immunoglobulin M levels in the breast milk of mothers with exclusive breastfeeding compared to mothers with non‐exclusive breastfeeding during 6 months postpartum: The Jordanian cohort study

Abuidhail

Al‐Shudiefat

Darwish

2018

American J Hum Biol

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Objectives The purpose of this study was to measure changes in the concentration of immunoglobulin G (IgG) and immunoglobulin M (IgM) in the mature breast milk of Jordanian mothers during the first 6 months after giving birth between exclusively breastfeeding (EBF) mothers and non‐exclusively breastfeeding (non‐EBF) mothers. Methods A longitudinal follow‐up design was used to measure changes in the concentration of IgG and IgM in the mothers' mature milk during the first 6 months after giving birth. Sixty‐nine lactating mothers were recruited in this study. Breast milk samples were collected by mothers themselves in the first, fourth, and sixth months of lactation to measure IgG and IgM concentrations using the enzyme‐linked immunosorbent assay (ELISA) technique. Results There was a significant difference in IgG and IgM concentrations between EBF and non‐EBF mothers. IgG was higher in the EBF mothers' milk than in the milk of non‐EBF mothers, whereas IgM was not affected by the type of baby feeding. Conclusion The concentration of immunoglobulins changes in human breast milk along with breastfeeding intervals. EBF enhances the concentration of IgG in breast milk compared to non‐EBF.

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Section: Introductionmentioning

confidence: 99%

Alterations of immunoglobulin G and immunoglobulin M levels in the breast milk of mothers with exclusive breastfeeding compared to mothers with non‐exclusive breastfeeding during 6 months postpartum: The Jordanian cohort study

Abuidhail

Al‐Shudiefat

Darwish

2018

American J Hum Biol

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“…Recent studies show that colostrum may supplement the lack of antibodies in the newborn, since colostrum have high levels of IgA against S. mutans antigens. 20 Here, the differences found in the frequency and specificity of IgA between C and BS support the importance of breastfeeding against oral colonization early in life. Clearly, the high levels of IgA specific to S. mutans antigens in colostrum can address the lack of antibodies in the newborn's saliva, especially against GbpB, a protein which is absent in saliva of the absolute majority of babies' saliva samples.…”

Section: Discussionmentioning

confidence: 54%

“…The analysis of S. mutans-specific IgA antigens showed that the majority of samples with positive response to S. mutans presented IgA response against Ag I/II and Gtf, which was found in colostrum samples 20 and saliva from children at birth, 14 at three months 14 and 5-11 months of age, 12 regardless of S. mutans colonization. On the other hand, many samples had a negative IgA response to GbpB, especially in the salivary samples.…”

Section: Discussionmentioning

confidence: 97%

“…11,20 Patterns of reactivity of salivary IgA antibody against S. mutans (UA159) and S. mitis (ATCC506) antigens were determined in western blot assays as previously described. 20 Sixteen micrograms of antigen extracts were loaded per lane, separated by 6% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to nitrocellulose membranes. Membranes were washed and blocked overnight at 4°C (in Tris-buffered saline-Tween, pH 7.5, 5% nonfat milk, Sigma).…”

Section: Colostrum and Saliva Iga Reactivity Against Streptococcal Anmentioning

confidence: 99%

“…20 Therefore, colostrum provides to the neonate significant levels of specific IgA against S. mutans virulence, which can disrupt the installation and accumulation process of these microorganisms in the oral cavity 20 and supply the immature mucosal immune system in early life, especially against GbpB, as anti-GpbB IgA is absent at birth. 13,14 There is little information about the immune response during the early stages of S. mutans bacterial challenge and the role of IgA provided by breastfeeding against the installation of these bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Comparisons of IgA response in saliva and colostrum against oral streptococci species

et al. 2017

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The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies' samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate.

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Streptococcus mutans in Umbilical Cord Blood, Peripheral Blood, and Saliva from Healthy Mothers

et al. 2018

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The aims of this study were to analyze the presence of Streptococcus mutans (SM)-DNA in cord blood (CB), maternal peripheral blood (PB), and maternal saliva (SA) and compare with data collected in health surveys. Sixty-four healthy women with pregnancies to term and without complications attending for elective cesarean section in the Clinical Hospital of Ribeirao Preto, Sao Paulo were included. Samples of PB and unstimulated SA were obtained on the day of hospitalization and samples of CB were collected after the delivery section. Samples were investigated using polymerase chain reaction for the presence of SM-DNA using specific primers. The results show over 50% of the sample of PB and CB showed SM-DNA detectable. There was a positive correlation between the SM detection in PB/CB and SA (P < 0.05). Pregnant women, who reported tooth brushing more than three times a day, often showed detectable SM-DNA in PB and CB (P < 0.05). In conclusion, the majority of children can have contact with SM-DNA during the intrauterine life by the CB. SM probably transferred from salivary habitat to PB and CB. The tooth brushing can be associated to S. mutans detection in blood samples.

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Levels and complexity of IgA antibody against oral bacteria in samples of human colostrum

Cited by 13 publications

References 36 publications

Alterations of immunoglobulin G and immunoglobulin M levels in the breast milk of mothers with exclusive breastfeeding compared to mothers with non‐exclusive breastfeeding during 6 months postpartum: The Jordanian cohort study

Alterations of immunoglobulin G and immunoglobulin M levels in the breast milk of mothers with exclusive breastfeeding compared to mothers with non‐exclusive breastfeeding during 6 months postpartum: The Jordanian cohort study

Comparisons of IgA response in saliva and colostrum against oral streptococci species

Streptococcus mutans in Umbilical Cord Blood, Peripheral Blood, and Saliva from Healthy Mothers

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