Levels of gingival crevicular fluid matrix metalloproteinases in periodontally compromised teeth under orthodontic forces

Abstract

Objective: To examine levels of matrix metalloproteinases (MMPs)-1, -2, -3, -7, -8, -12, and -13 in the gingival crevicular fluid (GCF) of periodontally compromised teeth at different time points during orthodontic movement. Materials and Methods: Ten controlled periodontitis subjects were submitted to orthodontic treatment. One dental arch was subjected to orthodontic movement, and teeth in the opposite arch were used as controls. GCF samples were collected from the lingual sites of two movement and two control incisors 1 week before orthodontic activation (27 d), immediately after orthodontic activation, and after 1 hour, 24 hours, and 7, 14, and 21 days. Multiplexed bead immunoassay was used to measure MMPs in GCF. Data were analyzed using Friedman and Wilcoxon statistical tests. Results: The only significant change found over time was in the levels of MMP-1 in the movement group (P , .05). When the two groups were compared after activation, the only statistically significant difference found was in levels of MMP-12 24 hours after activation (P , .05). Conclusions: Our findings suggested that the orthodontic movement of periodontally compromised teeth without active pockets did not result in significant changes in the GCF levels of MMPs.

Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement

“…Matrix metalloproteinases (MMPs) are also involved in these remodelling processes and are mainly responsible for the breakdown of the extracellular matrix during orthodontic tooth movement [ 1 , 33 , 34 ]. Macrophages react to pressure application and hypoxia with an upregulation of Mmp-9 gene expression, which is known to be regulated by HIF-1 α [ 35 ].…”

Ortodontinio Gydymo Įtaka Pažeistiems Periodonto Audiniams

“…Periodontitu sergančių suaugusių pacientų periodonto audiniuose per pirmas šešias ortodontinio gydymo savaites pastebimas reikšmingas periodontologines ligas skatinančių patogenų Campylobacter rectus, Veilonella, Peptostreptococcus, Actinomyces viscosus, Bacterionema rothia skaičius, tačiau, esant tinkamai periodonto audinių sveikatos priežiūrai, jis išlieka stabilus ir aktyvios periodonto ligos nesukelia [9]. Kiti in vivo tyrimų autoriai taip pat pastebi, kad atsakingai kontroliuojant periodontitu sergančio paciento periodonto audinių būklę ortodontinio gydymo metu, uždegiminiai ir mikrobiologiniai pokyčiai nedaro neigiamo poveikio periodonto sveikatai: A. actinomycetemcomitans, P. gingivalis, T. forsythia ir T. dentincola bakterijų skaičius per pirmąjį ortodontinio gydymo pusmetį ir matrikso metalo proteinazių kiekis dantenų vagelės skystyje 21 dienos laikotarpiu reikšmingai nedidėja, o kraujavimas po zondavimo, ortodontinio gydymo pradžioje galintis siekti 90 proc., po 4-6 ortodontinio gydymo mėnesių sumažėja iki 20 procentų [10,11].…”

Kieferorthopädische Therapie im parodontal kompromittierten Gebiss – Herausforderungen, interdisziplinäre Therapie und Grenzen

“…Bei korrekter Kraftapplikation und Beachtung der veränderten Biomechanik sowie guter Mundhygiene muss nach Ausheilung aktiver Entzündungsprozesse kein progredienter Attachmentverlust bei einer kieferorthopädischen Therapie im parodontal vorgeschädigten Gebiss befürchtet werden [14][15][16]. Almeida et al konnten dies auch auf molekularer Ebene bestätigen [17]. Hierbei wurde nachgewiesen, dass bei der orthodontischen Bewegung von parodontal kompromittierten, aber entzündungsfreien Zähnen, keine signifikant erhöhte Produktion von gewebeabbauenden Paramatern feststellbar war [17].…”