Levels of Thyroid and Thyrotrophic Hormones in the Blood of Friesian Cows at Various Reproductive Stages

Soliman, F. A.; Nasr, Hmed Ben; Zaki, K.

doi:10.1530/jrf.0.0060335

Cited by 16 publications

(13 citation statements)

References 13 publications

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“…1, Large cornified cells (longitudinal diameter greater than 60 p). Therefore, on the basis of the hormonal-vaginal epithelium relationships, the results obtained in this investigation could be explained as follows: During the early stages of pregnancy the progesterone level overrides that of oestrogens in cows ( SOLIMAN et al, 1964), and the vaginal epithelial cells are small, oval and violet. O n the other hand, during the last stages of pregnancy oestrogen exceeds progesterone and the vaginal epithelial cells are large, cornified and red.…”

Section: Discussionmentioning

confidence: 74%

Changes in the Cell Pattern in Vaginal Smears of Cattle During Pregnancy

Abdo

Ayoub

El‐Dakhakhni

2010

Zentralblatt Für Veterinärmedizin Reihe A

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Summary A technique was developed by which the different stages of gestation in cows could be diagnosed. This technique depends on taking smears from the dorsal vaginal vestibule with the edge of a slide, spreading the material on another slide and staining by Wright's Leishman stain. Evaluation of the results depends on the classification of the cells in the stained smears according to their size, shape and colour i.e. 1. Small, oval, violet. 2. Intermediate, neutral. 3. Large, cornified, red. 4. Vacuolated. The ratio of these different cells to each other was calculated, tabulated and expressed as a percentage of the total number of epithelial cells in each smear at each month of gestation. Zusammenfassung Veränderungen an den Scheidenepithelzellen von Kühen während der Gravidität Die Autoren beschreiben eine Methode zur Diagnose verschiedener Trächtigkeitsstadien bei Kühen. Es wird dem dorsalen Scheidewandvorhof ein Abstrich mit der scharfen Kante eines Objektträgers entnommen, das Material auf einen zweiten Objektträger gebracht und nach Wright angefärbt. Die Scheidenepithelien werden nach Größe, Form und Färbbarkeit der Zellen beurteilt und dabei vier Typen von Epithelzellen unterschieden: 1. kleine, ovale, basophile. 2. mittelgroße, neutrophile. 3. große, verhornte, acidophile. 4. Zellen mit Vakuolen. Das prozentuale Verhältnis dieser Zellen eines Scheidenabstriches wurde für jeden Trächtigkeitsmonat festgehalten. Résumé Modifications des cellules épithéliales du vagin de la vache au cours de la gestation Les auteurs décrivent une méthode pour diagnostiquer les différents stades de la gestation chez la vache. On prélève un frottis dans le vestibule de la vulve, à l'aide de la tranche d'un porte‐objet, on glisse le matériel sur un deuxième porte‐objet et on procède à une coloration selon Wright. On différencie les cellules épithéliales du vagin d'après leur grandeur, leur forme et leur coloration. On distingue quatre types: 1. le petites, ovales, basophiles, 2. les moyennes, neutrophiles, 3. les grosses, cornées, acidophiles, 4. les cellules à vacuoles. On détermine le pourcentage de ces cellules dans les frottis, pour chaque mois de la gestation. Resumen Modificaciones en las células del epitelio vaginal de vacas durante la gestación Se desarrolló una técnica, con la cual se pueden diagnosticar los estadios diferentes de gestación en vacas. El procedimiento consiste en tomar un frotis del vestíbulo dorsal de la vagina con el borde cortante de un portaobjetos, pasar el material a un segundo porta y teñir según Wright. Los epitelios vaginales se enjuician con arreglo al tamaño, forma y colorabilidad, diferenciándose cuatro tipos de células epiteliales: 1° pequeñas, ovales, basófilas; 2° intermedias, neutrófilas; 3° grandes, cornificadas, acidófilas; 4° vacuoladas. Para cada mes de gestación se computó la relación porcentual de estas células en los frotis vaginales.

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Section: Discussionmentioning

confidence: 74%

Changes in the Cell Pattern in Vaginal Smears of Cattle During Pregnancy

Abdo

Ayoub

El‐Dakhakhni

2010

Zentralblatt Für Veterinärmedizin Reihe A

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“…Previous reports on the levels of oestrogens in the peripheral plasma of cyclic dairy cows have been inconsistent. For example, levels of 0-1 ng oestrone equivalent/100 ml (Pope et al 1965), 300 ng oestradiol equivalent/100 ml (Ayalon & Lewis, 1961), 6100 ng oestradiol equivalent/100 ml (Soliman et al 1964) and 660-3600 ng total oestrogens/ 100 ml (Higaki, Suga & Fujisaki, 1959) have been reported for animals at oestrus. Oestrone concentrations found in bovine jugular blood during gestation using the present method (Robinson et al 1970) have been, as expected, considerably higher than those reported here.…”

Section: Resultsmentioning

confidence: 99%

“…Several authors (Szego & Roberts, 1946;Bitman, Wrenn & Sykes, 1958;Saba, 1964;Soliman, Nasr, Abdo, Zaki & Soliman, 1964;Pope, Jones & Waynforth, 1965;Robinson, Baker, Anastassiadis & Common, 1970) have studied oestrogenic activity in bovine blood during gestation. In addition, oestrogens have been demonstrated in urine during the oestrous cycle of the cow (Mellin & Erb, 1966;Erb, Randel & Callahan, 1971; Garverick, Erb, Niswender & Callahan, 1971).…”

Section: Introductionmentioning

confidence: 99%

“…However, studies on the concentrations of oestrogens in the peripheral blood of the dairy cow during the oestrous cycle have been few and the results inconsistent. Soliman et al (1964), using a mouse uterine weight assay, found 34-3 and 61-0/¿g oestradiol equivalent/1 during dioestrus and oestrus, respectively. Ayalon & Lewis (1961) using a similar procedure, detected 3 µg oestradiol-17/?…”

Section: Introductionmentioning

confidence: 99%

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Oestrone and Oestradiol in Jugular Vein Plasma During the Oestrous Cycle of the Cow

Mason¹,

Krishnamurti²,

Kitts³

1972

Journal of Endocrinology

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The concentrations of oestrone and oestradiol in jugular vein plasma during the bovine oestrous cycle were measured using a fluorescence assay. The highest levels of both oestrogens were found on the 19th and 20th days of a standardized 21-day cycle, with the peak of oestrone occurring slightly before that of oestradiol. Oestrone values ranged from 23\m=.\1 to 226\m=.\9ng/ 100ml and oestradiol varied between 17\ m=. \ 6 and 117\m=.\5ng/100 ml. Relatively high values for oestrone concentrations were found on days 6, 7 and 8. These values are comparable to those observed in the ovarian vein blood of the ewe, using both the present method and radioimmunoassay.The relationship between the oestrogen concentrations reported here and the levels of other hormones during the bovine oestrous cycle is discussed.

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“…One can present the hypothesis that the increase of TSH amount at the 15th day playa role in regression of the corpora lutea. It has been reported that TSH level is increased in the serum during diestrus in cows (Soliman et al 1963) and buffaloes (Abdo 1962).…”

Section: Discussionmentioning

confidence: 98%

Hormonal Changes During Formation and Involution of Corpus Luteum in Rabbits

Labib¹,

Azab²,

Soliman³

1978

Acta Vet. Brno

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Labib F., E. A. El Azab, F. A. Soliman: Hormonal Changes During Formation and Involution of Corpus Luteum in Rabbits. Acta vet. Brno, 47, 1978: 23-31. A study was made on 30 female Boscat rabbits to reveal the hormonal changes, namely, TSH, FSH, LH and L TH during the formation, maintenance and involution of the corpora lutea. Induction of ovulation was done by using gonadotrophic hormones (PMS and HCG). After HCG injection the animals were devided into 3 groups of 10 animals each. They were slaughtered after 3, 7 and 15 days from HCG injection. The results observed from this experiment are as follows: 3-day old corpus luteumThe rate of synthesis and release of TSH and state of thyroid were considered in a stabilized, balanced euthyroid status. There was increased concentration of FSH in the pituitaries with a relatively low level in the blood. This indicates the inhibition of release of FSH at this stage of development of corpora lutea.The level of L TH in the pituitary was of an average value and low in the serum. 7-days old corpus luteumThe TSH content of the pituitaries was the lowest (decreased synthesis) with a mild change in its level in the blood (non-significant). The thyroids were relatively less active than in the previous group.The FSH content of the pituitaries and blood were significantly decreased (decreased synthesis and release of FSH). The LH content of the pituitaries was increased and the level in the blood was decreased (decreased release of LH).The L TH content of the pituitaries was relatively low, while that of the blood was maximal, indicating increased release of L TH. IS-day old corpus luteumThe TSH content of the pituitaries and blood were highest indicating increased synthesis and release of this hormone. The thyroid gland showed maximum activity as compared to the previous groups.The FSH content of the pituitaries was relatively low while it reached its maximum in the serum (increased rate of release of FSH). The level of LH in the pituitaries was low and that of the blood was the least. The L TH content of the pituitaries was at its maximum and it was the lowest in the blood (increased synthesis of L TH but blocked release into the blood). The results are discussed in the text. LTH, TSH, LH, FSH, pituitary, blood serum.The features of growth and maintenance of the corpus luteum, and its subsequent involution are active processes of extreme importance in regulating reproductive performance of mammals. This includes regulation of the estrous cycle, pregnancy, parturition and milk production.Any disturbance of such processes of the corpus luteum will lead to reproductive disturbances in the farm animals.

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Levels of Thyroid and Thyrotrophic Hormones in the Blood of Friesian Cows at Various Reproductive Stages

Cited by 16 publications

References 13 publications

Changes in the Cell Pattern in Vaginal Smears of Cattle During Pregnancy

Changes in the Cell Pattern in Vaginal Smears of Cattle During Pregnancy

Oestrone and Oestradiol in Jugular Vein Plasma During the Oestrous Cycle of the Cow

Hormonal Changes During Formation and Involution of Corpus Luteum in Rabbits

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