Leydig Cell Steroidogenesis: Unmasking the Functional Importance of Mitochondria

Haider, Syed G.

doi:10.1210/en.2007-0330

Cited by 31 publications

(14 citation statements)

References 11 publications

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“…In mice, the fetal Leydig cells are present and observable as round to oval shaped and are found exclusively in clusters. Leydig cells start to regress postnatally marked by the scattering of clusters and their decreasing number2831. The histological examination of testis cross-sections stained by the Leydig cell marker, 3β-HSD (3-β-Hydroxysteroid Dehydrogenase/Δ-5-4 isomerase), revealed no detectable size or quantitative differences of fetal Leydig cell clusters between FS and FD males at PND6 (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Low paternal dietary folate alters the mouse sperm epigenome and is associated with negative pregnancy outcomes

et al. 2013

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Epidemiological studies suggest that a father’s diet can influence offspring health. A proposed mechanism for paternal transmission of environmental information is via the sperm epigenome. The epigenome includes heritable information such as DNA methylation. We hypothesize that the dietary supply of methyl donors will alter epigenetic reprogramming in sperm. Here we feed male mice either a folate-deficient or folate-sufficient diet throughout life. Paternal folate deficiency is associated with increased birth defects in the offspring, which include craniofacial and musculoskeletal malformations. Genome-wide DNA methylation analysis and the subsequent functional analysis identify differential methylation in sperm of genes implicated in development, chronic diseases such as cancer, diabetes, autism and schizophrenia. While >300 genes are differentially expressed in offspring placenta, only two correspond to genes with differential methylation in sperm. This model suggests epigenetic transmission may involve sperm histone H3 methylation or DNA methylation and that adequate paternal dietary folate is essential for offspring health.

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Section: Resultsmentioning

confidence: 99%

Low paternal dietary folate alters the mouse sperm epigenome and is associated with negative pregnancy outcomes

et al. 2013

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“…Binding of LH to its receptor in a Leydig cell initiates a series of events that include an increase in intracellular cyclic adenosine monophosphate (cAMP), translocation of cholesterol into mitochondria, conversion of cholesterol to pregnenolone, translocation of pregnenolone into the smooth endoplasmic reticulum and the conversion of pregnenolone to testosterone through a cascade of reactions that are catalysed by the cytochrome P-450 family of proteins. 65 The process of steroidogenesis itself can serve as a source of ROS. 66 The products that are formed during normal steroidogenesis can act as pseudosubstrates and interact with P-450 enzymes, resulting in the formation of a pseudosubstrate-P-450-O 2 complex, which is a source of damaging free radicals due to the inability of the pseudosubstrate to undergo hydroxylation.…”

Section: Ros Generation and Apoptosis In Steroidogenesismentioning

confidence: 99%

The effect of environmental contaminants on testicular function

Mathur¹,

D’Cruz²

2011

Asian J Androl

162

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“…By binding to the membrane LH receptor, LH induces cAMP synthesis in Leydig cells. cAMP catalyzes PKA synthesis, and PKA transports cholesterol from the cytoplasmic pool to the mitochondria to promote steroidogenesis by steroidogenic enzymes (CYP11A1, HSD3B, and so on) and StAR [12, 13]. Additionally, steroidogenesis is also dependent on the number and activity of Leydig cells [13].…”

Section: Introductionmentioning

confidence: 99%

Effects of the Yangjing Capsule Extract on Steroidogenesis and Apoptosis in Mouse Leydig Cells

Sun

Cui²,

Jin³

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Objectives. This study aimed to explore the effect and mechanism of Yangjing capsule on testosterone secretion in mouse Leydig tumor cells (MLTC-1). Methods. MLTC-1 cells were treated with the Yangjing capsule extract for 24 h. The testosterone level in medium was measured by radioimmunoassay. The expression of steroidogenic enzymes (StAR, CYP11A1, and HSD3B) in the cells was examined using real-time RT-PCR and immunoblotting. Additionally, MLTC-1 cells were treated for 48 h in a serum-free medium. The cell viability was measured by MTT assay. The cell cycle and apoptosis were analyzed using flow cytometry. The expression of activated caspase-3 was analyzed using RT-PCR and a colorimetric protease assay. Results. The Yangjing capsule extract increased testosterone production and the expression of StAR, CYP11A1, and HSD3B mRNAs and proteins compared with the control. H89 significantly inhibited these effects. The medicine improved the viability of MLTC-1 cells, decreased the number of cells in G0/G1 phase, and increased the number of cells in S-phase, as well as prevented cell apoptosis by inhibiting caspase-3. Conclusion. The Yangjing capsule can stimulate MLTC-1 cells to secrete testosterone and may be an alternative treatment for diseases characterized by insufficient testosterone production.

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Leydig Cell Steroidogenesis: Unmasking the Functional Importance of Mitochondria

Cited by 31 publications

References 11 publications

Low paternal dietary folate alters the mouse sperm epigenome and is associated with negative pregnancy outcomes

Low paternal dietary folate alters the mouse sperm epigenome and is associated with negative pregnancy outcomes

The effect of environmental contaminants on testicular function

Effects of the Yangjing Capsule Extract on Steroidogenesis and Apoptosis in Mouse Leydig Cells

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