LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus

Abstract

In innate immunity, LGP2 (laboratory of genetics and physiology 2) plays a very important role in the production of type I interferon (IFN) through recognition of cytosolic viral RNA. Although viral infection or stimulation with double-strand RNA dramatically induces expression of the LGP2 gene, the underlying transcriptional mechanism has never been studied. Here, we cloned and characterized the 5′-upstream region (−1,337 bp) of the LGP2 gene in olive flounder (Paralichthys olivaceus). Numerous canonical motifs for IFN-regulatory factors (IRFs) were found in this region, and reporter assays identified a poly I:C-responsive promoter region (−506 to −398) that regulated LGP2 transcription. Transcriptional activity of the LGP2 promoter was strongly enhanced by IRF3, which bound to IRF3 motif #3 (−480). The LGP2 promoter was also responsive to viral infection in vitro. These results suggest that LGP2 transcriptional control is crucially involved to regulated by IRF3 function after viral infection or stimulation with poly I:C.

Identification and expression of the laboratory of genetics and physiology 2 gene in common carp Cyprinus carpio

“…olivaceus , C . idella and O. mykiss , expression levels of the lgp2 gene and type I ifn gene were dramatically induced by poly(I:C) stimulation and viral infection (Ohtani et al , ; Huang et al , ; Chang et al , ; Hikima et al , ). There is conflicting evidence, however, for example, in C .…”

“…In bony fishes such as P. olivaceus, C. idella and O. mykiss, expression levels of the lgp2 gene and type I ifn gene were dramatically induced by poly(I:C) stimulation and viral infection (Ohtani et al, 2010;Huang et al, 2010;Chang et al, 2011;Hikima et al, 2012). There is conflicting evidence, however, for example, in C. auratus that lgp2 inhibits poly(I:C)-induced activation of D. rerio type I ifn production (Sun et al, 2011).…”

Innovation in NLR and TLR sensing drives the MHC-II free Atlantic cod immune system

“…DEGs from both challenge groups revealed 46 up-regulated genes expressed in both treatments (Fig.2C, Table S2, Data S1). Interestingly, cod, flounder (Paralichthys olivaceus) (22), chicken (Gallus gallus) (23) and the tree shrew (Tupaia chinensis) ( Table S3). Synteny in the teleost lineage was less conserved for ifih1-adjacent genes including fap, gcg and dpp4, which displayed significant variation.…”

Temperature-dependent regulation of gene expression in poly (I:C)-treated Japanese flounder, Paralichthys olivaceus

“…2). Japanese flounder IRF3 regulates the transcription of type I IFN and laboratory of genetics and physiology 2 (LGP2) after viral infection or poly (I:C) treatment [37,38]. Hu et al [35] demonstrated that IRF3 expression in Japanese flounder reared at 18 C and treated with poly (I:C) could be detected at 1e4 days post injection (dpi) and then declined at 5e7 dpi.…”