Licochalcone A from licorice root, an inhibitor of human hepatoma cell growth via induction of cell apoptosis and cell cycle arrest

Wang, Jun; Zhang, Ying-Shuo; Thakur, Kiran; Hussain, Sayed Sajid; Zhang, Jianguo; Xiao, Guiran; Wei, Zhao‐Jun

doi:10.1016/j.fct.2018.07.044

Cited by 96 publications

(55 citation statements)

References 41 publications

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“…Although these results indicate that the low-Mw polysaccharides in the present study have a promising in vitro antiproliferative effect on cancer cell lines, validation of their antitumor effect and evaluation of toxicity are required in further studies. Also, previous studies have demonstrated that the upregulation of P53 and P21, together with the downregulation of Cyclin B1 and Cdk1, serve important roles in blocking the cell cycle, which is the potential antitumor mechanism of a variety of clinical anticancer medicines (37)(38)(39)(40). The present data demonstrate that PYSP-20 and PYSP-100 are able to regulate the expression of P53, P21, Cyclin B1 and Cdk1 and so may induce cell cycle arrest.…”

Section: Discussionsupporting

confidence: 68%

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain Pyropia yezoensis Sookwawon 104 with in vitro antiproliferative activity

Yan

et al. 2020

Oncol Lett

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Pyropia yezoensis Sookwawon 104 is a newly cultivated strain of red marine algae. The present study aimed to investigate the in vitro antiproliferative activity of sulfated polysaccharide extracted from P. yezoensis Sookwawon 104 (PYSP), as well as that of its low molecular weight (Mw) derivatives. PYSP is a heterogeneous sulfated polysaccharide mainly composed of galactose, glucose and fucose. PYSP was degraded by gamma-irradiation at doses of 20 and 100 kGy to produce two derivatives, named as PYSP-20 and PYSP-100, respectively. Comparison of PYSP, PYSP-20 and PYSP-100 revealed clear differences in their molecular weight (Mw) distributions, and distinct in vitro antiproliferative activities against Hep3B, MDA-MB-231 and HeLa cancer cell lines. PYSP-20 and PYSP-100 exhibited stronger antiproliferative effects than PYSP, suggesting that the reduction in Mw may have increased the in vitro antiproliferative activity. Furthermore, the mRNA expression levels of the antitumor gene P53 and cell cycle-associated genes P21, Cyclin B1 and cyclin dependent kinase 1 (Cdk1) were further analyzed by reverse transcription-quantitative PCR in PYSP-20 and PYSP-100-treated cancer cells. PYSP and its derivatives were shown to inhibit the proliferation of tumor cells by regulating the expression of P53, P21, Cyclin B1 and Cdk1. In conclusion, low-Mw polysaccharide derivatives prepared from P. yezoensis Sookwawon 104 by gamma-irradiation exhibit significant inhibition effects on cancer cell proliferation in vitro and may be a novel source of potential anticancer therapeutic agents.

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Section: Discussionsupporting

confidence: 68%

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain Pyropia yezoensis Sookwawon 104 with in vitro antiproliferative activity

Yan

et al. 2020

Oncol Lett

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“…Previously, Niu et al reported that LCA led to high production of ROS, thereby inducing autophagy in human hepatocellular carcinoma cells, and inhibition of LCA-induced ROS by NAC participated in LCA-mediated apoptosis [12]. Wang et al found that LCA played an anticancer role in human hepatoma cells through enhancing G2/M phase arrest and apoptosis [13]. These findings suggested the different regulatory functions of LCA in different cancer cells.…”

Section: Biomed Research Internationalmentioning

confidence: 99%

Licochalcone a Induces ROS-Mediated Apoptosis through TrxR1 Inactivation in Colorectal Cancer Cells

Yu²,

et al. 2020

BioMed Research International

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Licochalcone A (LCA) exhibited anticancer activity through modulating reactive oxygen species (ROS) levels in some cancer cells and has been evidenced to suppress colorectal cancer (CRC) formation and progression. However, whether LCA mediates the progression of CRC by regulating ROS production remains unclear. To address this, HCT-116 cells were treated with LCA, resulting in G0/G1 phase arrest, apoptosis, and high ROS generation, which were attenuated by N-acetyl-L-cysteine, a ROS inhibitor. In addition, LCA suppressed the expression of thioredoxin reductase 1 (TrxR1) in HCT-116 cells, leading to high ROS levels and apoptosis. Moreover, LCA administration combined with TrxR1 inhibition further enhanced the production of ROS and apoptosis in HCT-116 cells compared to LCA administration or TrxR1 inhibition alone. These results demonstrated that LCA might enhance the production of ROS by targeting TrxR1, leading to apoptosis in HCT-116 cells, which provides potential insight for the interventional treatment of CRC.

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“…Cyclin B1 and cyclin-dependent kinase 1 (CDK1) were taken as the arresting G2/M checkpoint, with CDKs being potential cancer therapeutic targets. 56,57 The cell cycle arrest was related to the upregulation of inhibitors p53 and p21. 58 Moreover, p53 also plays a key role in anticancer effects.…”

Section: Effect Of Porphyrans On Gene Expression Of the Cell Cycle Inmentioning

confidence: 99%

Antitumor bioactivity of porphyran extracted from Pyropia yezoensis Chonsoo2 on human cancer cell lines

Yan

et al. 2019

J Sci Food Agric

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BACKGROUND Pyropia yezoensis, rich in porphyran, is a medicine‐edible red alga. In the present study, the physicochemical characteristics, conformational states and antitumor activities of a novel porphyran extracted from the high‐yield algal strain Pyropia yezoensis Chonsoo2 and its two degraded derivatives by gamma irradiation were investigated. RESULTS Pyropia yezoensis porphyran is a water‐soluble, triple‐helical sulfated hetero‐galactopyranose, named PYP. PYP was degraded by gamma irradiation at 20 kGy and 50 kGy, giving two low molecular weight derivatives comprising PYP‐20 and PYP‐50, respectively. PYP with a higher molecular weight has a solution conformation different from PYP‐20 and PYP‐50. Three porphyrans had no toxicity in normal human liver cells (HL‐7702) and showed antitumor effects on Hep3B, HeLa and MDA‐MB‐231. They had better antitumor against HeLa cells, exhibiting a similar inhibition ratio compared to 5‐fluorouracil, with PYP especially exhibiting a higher inhibition ratio than 5‐fluorouracil. With respect to HeLa cells, the different antitumor activities might be related to porphyran molecular weight and solution conformation. Furthermore, the HeLa cell cycle was blocked in the G2/M phase after PYP treatment, leading to cell proliferation inhibition. The induction of cell cycle arrest was related to the changes in the expression of p21, p53, Cyclin B1 and cyclin‐dependent kinase 1. CONCLUSION Pyropia yezoensis porphyran, as applied to medicine and functional food, could potentially be used as a non‐toxic natural adjuvant in cancer therapy. © 2019 Society of Chemical Industry

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Licochalcone A from licorice root, an inhibitor of human hepatoma cell growth via induction of cell apoptosis and cell cycle arrest

Cited by 96 publications

References 41 publications

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia yezoensis</em> Sookwawon 104 with <em>in vitro</em> antiproliferative activity

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia yezoensis</em> Sookwawon 104 with <em>in vitro</em> antiproliferative activity

Licochalcone a Induces ROS-Mediated Apoptosis through TrxR1 Inactivation in Colorectal Cancer Cells

Antitumor bioactivity of porphyran extracted from Pyropia yezoensis Chonsoo2 on human cancer cell lines

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Licochalcone A from licorice root, an inhibitor of human hepatoma cell growth via induction of cell apoptosis and cell cycle arrest

Cited by 96 publications

References 41 publications

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia&nbsp;yezoensis</em> Sookwawon&nbsp;104 with <em>in&nbsp;vitro</em> antiproliferative activity

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia&nbsp;yezoensis</em> Sookwawon&nbsp;104 with <em>in&nbsp;vitro</em> antiproliferative activity

Licochalcone a Induces ROS-Mediated Apoptosis through TrxR1 Inactivation in Colorectal Cancer Cells

Antitumor bioactivity of porphyran extracted from Pyropia yezoensis Chonsoo2 on human cancer cell lines

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Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia yezoensis</em> Sookwawon 104 with <em>in vitro</em> antiproliferative activity

Gamma‑irradiation degraded sulfated polysaccharide from a new red algal strain <em>Pyropia yezoensis</em> Sookwawon 104 with <em>in vitro</em> antiproliferative activity