Lidocaine Administration Controls MicroRNAs Alterations Observed After Lung Ischemia–Reperfusion Injury

Rancán, Lisa; Simón, Carlos; Marchal-Duval, Emmeline; Casanova, Javier; Paredes, Sergio D.; Calvo, Amelia Serraller; Garcı́a, Cruz; Rincón, David Nogales; Turrero, Agustı́n; Garutti, Ignacio; Vara, Elena

doi:10.1213/ane.0000000000001633

Cited by 34 publications

(27 citation statements)

References 41 publications

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“…In response to lidocaine treatment, the cells expressed different miRNAs patterns, possibly via which lidocaine exerted its specific function [24,25]. To date, several miRNAs have been mentioned as the effector genes of lidocaine, such as miR-520-3p [22], miR-493 [26], and miR-let-7b [27].…”

Section: Discussionmentioning

confidence: 99%

Lidocaine inhibits proliferation and metastasis of lung cancer cell via regulation of miR-539/EGFR axis

Sun

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background: Despite the medical uses of lidocaine has been well-characterized, the study of lidocaine's pharmacological function other the anaesthetic effect was never stopped. This study designed to reveal the effect of lidocaine on the growth and metastasis of lung cancer in vitro. Methods: A549 and NCI-H1299 cells were treated by lidocaine for 24 h. miR-539 expression in cell was silenced by transfection with the specific inhibitor. The changes in cell growth and metastasis were determined using CCK-8 assay and western blot. Luciferase activity assay was performed to assay if EGFR was a target of miR-539. Western blot was used to test the activation of EGFR downstream signalling. Results: Lidocaine suppressed the viability, migration, and invasion of A549 and NCI-H1299 cells while induced apoptotic death. Lidocaine elevated the expression of miR-539. The anti-tumour properties of lidocaine towards A549 and NCI-H1299 cells were partially attenuated when miR-539 was silenced. EGFR was a target of miR-539. Lidocaine repressed the activation of ERK and PI3K/AKT pathways also via regulating miR-539. Conclusion: The anti-growth and anti-metastatic effects of lidocaine towards lung cancer cells. The anti-tumour properties of lidocaine may be partial via up-regulation of miR-539, which blocked EGFR signalling by directly binding with EGFR.

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Section: Discussionmentioning

confidence: 99%

Lidocaine inhibits proliferation and metastasis of lung cancer cell via regulation of miR-539/EGFR axis

Sun

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…There was no difference between the control and lidocaine groups in the duration of the ischaemic period (123.3 ± 8.16 min and 127.5 ± 8.89 min, respectively). Haemodynamic parameters were stable during the entire procedure, confirming previous results [24].…”

Section: Resultssupporting

confidence: 89%

“…First, we used juvenile animals which could present an inflammatory response slightly different from that in adults. However, the main concern when using young animals is about the haemodynamic stability which we observed remained stable during the experiments [24]. Other studies investigating the inflammatory response observed after lung surgery used similar animals [39].…”

Section: Discussionmentioning

confidence: 78%

“…As previously described [24], after the induction, the femoral vein and artery were catheterized with an Arrow-Howes 7-french triple-lumen catheter and a PiCCO thermodilution catheter (PV2014L16 femoral artery in small adults Ø 4-french, 16 cm in length), respectively. Main haemodynamic variables, fluid responsiveness, pre-load, after-load, contractility, and pulmonary oedema were measured with the PiCCO-Pulsion thermodilution monitor.…”

Section: Haemodynamic Measurementsmentioning

confidence: 99%

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Glycocalyx Degradation after Pulmonary Transplantation Surgery

et al. 2018

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Purpose: Ischaemia-reperfusion injury (IRI) is a main cause of morbidity after pulmonary resection surgery. The degradation of glycocalyx, a dynamic layer of macromolecules at the luminal surface of the endothelium, seems to participate in tissue dysfunction after IRI. Lidocaine has a proven anti-inflammatory activity in several tissues but its modulation of glycocalyx has not been investigated. This work aimed to investigate the potential involvement of glycocalyx in lung IRI in a lung auto-transplantation model and the possible effect of lidocaine in modulating IRI. Methods: Three groups (sham-operated, control, and lidocaine), each consisting of 6 Large White pigs, were subjected to lung auto-transplantation. All groups received the same anaesthesia. In addition, the lidocaine group received a continuous IV administration of lidocaine (1.5 mg/kg/h). Lung tissue and plasma samples were taken before pulmonary artery clamp, before reperfusion, and 30 and 60 min post-reperfusion in order to analyse pulmonary oedema, glycocalyx components, adhesion molecules, and myeloperoxidase level. Results: Ischaemia caused pulmonary oedema, which was greater after reperfusion. This effect was accompanied by decreased levels of syndecan-1 and heparan sulphate in the lung samples, together with increased levels of both glycocalyx components in the plasma samples. After reperfusion, neutrophil activation and the expression of adhesion molecules were increased. All these alterations were significantly lower or absent in the lidocaine group. Conclusion: Lung IRI caused glycocalyx degradation that contributed to neutrophil activation and adhesion. The administration of lidocaine was able to protect the lung from glycocalyx degradation.

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“…Lidocaine may provide protection against IR injury by preventing miRNA dysregulation. A recent study found that lung IR injury caused miRNA dysregulation, while lidocaine reduced these changes [78]. Further studies should be conducted to examine how lidocaine regulates miRNA expression.…”

Section: Regional Anesthesiamentioning

confidence: 99%

A Review of Anesthetic Effects on Renal Function: Potential Organ Protection

et al. 2017

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Background: Renal protection is a critical concept for anesthesiologists, nephrologists, and urologists, since anesthesia and renal function are highly interconnected and can potentially interfere with one another. Therefore, a comprehensive understanding of anesthetic drugs and their effects on renal function remains fundamental to the success of renal surgeries, especially transplant procedures. Some experimental studies have shown that some anesthetics provide protection against renal ischemia/reperfusion (IR) injury, but there is limited clinical evidence. Summary: The effects of anesthetic drugs on renal failure are particularly important in the context of kidney transplantation, since the conditions of preservation following removal profoundly influence the recovery of organ function. Currently, preservation procedures are typically based on the usage of a cold-storage solution. Some anesthetic drugs induce anti-inflammatory, anti-necrotic, and anti-apoptotic effects. A more thorough understanding of anesthetic effects on renal function can present a novel approach for developing organ-protective strategies. The aim of this review is to discuss the effects of different anesthetic drugs on renal function, with particular focus on IR injury. Many studies have demonstrated the organ-protective effects of some anesthetic drugs, specifically propofol, which indicate the potential of some anesthetics to introduce novel organ protective targets. This is not surprising, since lipid emulsions are major components of propofol, which accumulating data show provide organ protective effects against IR injury. Key Messages: Thorough understanding of the interaction between anesthetic drugs and renal function remains fundamental to the delivery of safe perioperative care and to optimizing outcomes after renal surgeries, particularly transplant procedures. Anesthetics can be repurposed for organ protection with more information about their effects, especially during transplant procedures. Here, we review the effects of different anesthetic drugs – specifically those that contain lipids in their structure, with special reference to IR injury.

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Lidocaine Administration Controls MicroRNAs Alterations Observed After Lung Ischemia–Reperfusion Injury

Abstract: Lung IRI caused dysregulation miRNA. The administration of lidocaine reduced significantly miRNAs alterations.

Cited by 34 publications

References 41 publications

Lidocaine inhibits proliferation and metastasis of lung cancer cell via regulation of miR-539/EGFR axis

Lidocaine inhibits proliferation and metastasis of lung cancer cell via regulation of miR-539/EGFR axis

Glycocalyx Degradation after Pulmonary Transplantation Surgery

A Review of Anesthetic Effects on Renal Function: Potential Organ Protection

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