Life in Hot Spring Microbial Mats Located in the Trans-Mexican Volcanic Belt: A 16S/18S rRNA Gene and Metagenomic Analysis

Prieto-Barajas, Cristina M.; Alcaraz, Luis David; Valencia-Cantero, Eduardo; Santoyo, Gustavo

doi:10.1080/01490451.2018.1454555

Cited by 13 publications

(5 citation statements)

References 59 publications

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“…Finally, the viruses identified correspond to archaeal Fusellovirus, archaeal Rudivirus, and Sulfolobales Archaeon AZ1 [30]. In addition, in a hot spring located in the Araro region, Michoacan, different genera of bacteria were found, such as Bacillus, Aeromonas, and Pseudomonas [31,32], whereas in the "Carrizal" thermal pool hot spring and in "Los Baños" in Veracruz, Mexico, bacteria of the genera Geobacillus, Anoxybacillus, and Aeribacillus have been identified [33].…”

Section: Introductionmentioning

confidence: 94%

The Microbial Composition in Circumneutral Thermal Springs from Chignahuapan, Puebla, Mexico Reveals the Presence of Particular Sulfur-Oxidizing Bacterial and Viral Communities

et al. 2020

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Terrestrial thermal springs are widely distributed globally, and these springs harbor a broad diversity of organisms of biotechnological interest. In Mexico, few studies exploring this kind of environment have been described. In this work, we explore the microbial community in Chignahuapan hot springs, which provides clues to understand these ecosystems’ diversity. We assessed the diversity of the microorganism communities in a hot spring environment with a metagenomic shotgun approach. Besides identifying similarities and differences with other ecosystems, we achieved a systematic comparison against 11 metagenomic samples from diverse localities. The Chignahuapan hot springs show a particular prevalence of sulfur-oxidizing bacteria from the genera Rhodococcus, Thermomonas, Thiomonas, Acinetobacter, Sulfurovum, and Bacillus, highlighting those that are different from other recovered bacterial populations in circumneutral hot springs environments around the world. The co-occurrence analysis of the bacteria and viruses in these environments revealed that within the Rhodococcus, Thiomonas, Thermonas, and Bacillus genera, the Chignahuapan samples have specific species of bacteria with a particular abundance, such as Rhodococcus erytropholis. The viruses in the circumneutral hot springs present bacteriophages within the order Caudovirales (Siphoviridae, Myoviridae, and Podoviridae), but the family of Herelleviridae was the most abundant in Chignahuapan samples. Furthermore, viral auxiliary metabolic genes were identified, many of which contribute mainly to the metabolism of cofactors and vitamins as well as carbohydrate metabolism. Nevertheless, the viruses and bacteria present in the circumneutral environments contribute to the sulfur cycle. This work represents an exhaustive characterization of a community structure in samples collected from hot springs in Mexico and opens opportunities to identify organisms of biotechnological interest.

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Section: Introductionmentioning

confidence: 94%

The Microbial Composition in Circumneutral Thermal Springs from Chignahuapan, Puebla, Mexico Reveals the Presence of Particular Sulfur-Oxidizing Bacterial and Viral Communities

et al. 2020

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“…Microbial mats are found across the planet in a variety of habitats [35][36][37][38][39][40]. Their laminated structure contains bacteria, archaea, and eukaryotes that work together, often symbiotically, sharing and cycling nutrients and energy [38,41]. Often driven by photosynthesis at their surface and chemosynthesis at their base, microbial mat structures are a key component of the living world that provide us with a snapshot of how microorganisms work together in a complex, ordered community to propagate and ensure their survival.…”

Section: Introductionmentioning

confidence: 99%

Insights into the dynamics between viruses and their hosts in a hot spring microbial mat

et al. 2020

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Our current knowledge of host–virus interactions in biofilms is limited to computational predictions based on laboratory experiments with a small number of cultured bacteria. However, natural biofilms are diverse and chiefly composed of uncultured bacteria and archaea with no viral infection patterns and lifestyle predictions described to date. Herein, we predict the first DNA sequence-based host–virus interactions in a natural biofilm. Using single-cell genomics and metagenomics applied to a hot spring mat of the Cone Pool in Mono County, California, we provide insights into virus–host range, lifestyle and distribution across different mat layers. Thirty-four out of 130 single cells contained at least one viral contig (26%), which, together with the metagenome-assembled genomes, resulted in detection of 59 viruses linked to 34 host species. Analysis of single-cell amplification kinetics revealed a lack of active viral replication on the single-cell level. These findings were further supported by mapping metagenomic reads from different mat layers to the obtained host–virus pairs, which indicated a low copy number of viral genomes compared to their hosts. Lastly, the metagenomic data revealed high layer specificity of viruses, suggesting limited diffusion to other mat layers. Taken together, these observations indicate that in low mobility environments with high microbial abundance, lysogeny is the predominant viral lifestyle, in line with the previously proposed “Piggyback-the-Winner” theory.

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“…The higher TN levels at Tramway Ridge could also be influenced by the lower pH present, which would trap ammonia gases as ammonium ( Holloway et al, 2011 ). Previous studies have suggested that moss beds and cyanobacterial mats increase the abundance and diversity of bacteria and other infauna relative to surrounding soils, which could account for the higher DNA yields observed at Tramway Ridge ( Carreira et al, 2015 ; Prieto-Barajas et al, 2018 ).…”

Section: Discussionmentioning

confidence: 97%

Unique Geothermal Chemistry Shapes Microbial Communities on Mt. Erebus, Antarctica

et al. 2022

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Mt. Erebus, Antarctica, is the world’s southernmost active volcano and is unique in its isolation from other major active volcanic systems and its distinctive geothermal systems. Using 16S rRNA gene amplicon sequencing and physicochemical analyses, we compared samples collected at two contrasting high-temperature (50°C–65°C) sites on Mt. Erebus: Tramway Ridge, a weather-protected high biomass site, and Western Crater, an extremely exposed low biomass site. Samples were collected along three thermal gradients, one from Western Crater and two within Tramway Ridge, which allowed an examination of the heterogeneity present at Tramway Ridge. We found distinct soil compositions between the two sites, and to a lesser extent within Tramway Ridge, correlated with disparate microbial communities. Notably, pH, not temperature, showed the strongest correlation with these differences. The abundance profiles of several microbial groups were different between the two sites; class Nitrososphaeria amplicon sequence variants (ASVs) dominated the community profiles at Tramway Ridge, whereas Acidobacteriotal ASVs were only found at Western Crater. A co-occurrence network, paired with physicochemical analyses, allowed for finer scale analysis of parameters correlated with differential abundance profiles, with various parameters (total carbon, total nitrogen, soil moisture, soil conductivity, sulfur, phosphorous, and iron) showing significant correlations. ASVs assigned to Chloroflexi classes Ktedonobacteria and Chloroflexia were detected at both sites. Based on the known metabolic capabilities of previously studied members of these groups, we predict that chemolithotrophy is a common strategy in this system. These analyses highlight the importance of conducting broader-scale metagenomics and cultivation efforts at Mt. Erebus to better understand this unique environment.

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Life in Hot Spring Microbial Mats Located in the Trans-Mexican Volcanic Belt: A 16S/18S rRNA Gene and Metagenomic Analysis

Cited by 13 publications

References 59 publications

The Microbial Composition in Circumneutral Thermal Springs from Chignahuapan, Puebla, Mexico Reveals the Presence of Particular Sulfur-Oxidizing Bacterial and Viral Communities

The Microbial Composition in Circumneutral Thermal Springs from Chignahuapan, Puebla, Mexico Reveals the Presence of Particular Sulfur-Oxidizing Bacterial and Viral Communities

Insights into the dynamics between viruses and their hosts in a hot spring microbial mat

Unique Geothermal Chemistry Shapes Microbial Communities on Mt. Erebus, Antarctica

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