Ligand fishing via a monolithic column coated with white blood cell membranes: A useful technique for screening active compounds in Astractylodes lancea

Zhang, Fan; Jiang, Yanlong; Jiao, Pan; Li, Shaoyong; Tang, Cheng

doi:10.1016/j.chroma.2021.462544

Cited by 6 publications

(3 citation statements)

References 56 publications

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“…Several ligand fishing methods have been reported, namely, monolithic column coated with white blood cell membranes ( 39 ), Hsp 90α functionalized InP/ZnS QDs embedded mesoporous nanoparticles ( 13 ), porcine pancreatic lipase immobilized organic framework UiO-66-NH 2 ( 40 ), and so on. A comparison between the previous ligand fishing method and this work was displayed in Table 3 .…”

Section: Resultsmentioning

confidence: 99%

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

et al. 2022

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The microfluidic platform is a versatile tool for screening and locating bioactive molecules from functional foods. Here, a layer-by-layer assembly approach was used to fabricate core-shell CdSSe@ZnS quantum dot encoded superparamagnetic iron oxide microspheres, which served as a carrier for matrix metalloproteinase-2. The matrix metalloproteinase-2 camouflaged magnetic microspheres was further incorporated into a homemade microfluidic platform and incubated with extracts of fruits of Rosa roxburghii. The flow rate of the microfluidic platform was tuned. The major influencing parameters on ligand binding, such as dissociate solvents, incubation pH, ion strength, temperature, and incubation time were also optimized by using ellagic acid as a model compound. The specific binding ligands were sent for structure elucidation by mass spectrometry. The absolute recovery of ellagic acid ranged from 101.14 to 102.40% in the extract of R. roxburghii under the optimal extraction conditions. The linearity was pretty well in the range of 0.009–1.00 mg·ml−1 (R2 = 0.9995). The limit of detection was 0.003 mg·ml−1. The relative SDs of within-day and between-day precision were <1.91%. A total of thirteen ligands were screened out from fruits of R. roxburghii, which were validated for their inhibitory effect by enzyme assay. Of note, eleven new matrix metalloproteinase-2 inhibitors were identified, which may account for the antitumor effect of fruits of R. roxburghii.

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Section: Resultsmentioning

confidence: 99%

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

et al. 2022

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“…Thereafter, CMs were immobilized on fitter paper, monolithic column, and so forth. These assays can be used to screen for the active compounds in NPs [13,14].…”

Section: Introductionmentioning

confidence: 99%

Chitosan‐based matrix solid‐phase dispersion extraction assisted cell membrane magnetic bead ligand‐affinity assay for screening active compounds from Fructus Cnidii

Jiao

Zhang

et al. 2022

J of Separation Science

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Cell membrane ligand-affinity assay is a useful tool for screening the active compounds from natural products. However, in traditional cell membrane ligand-affinity assays, natural products need to be refluxed, before being analyzed. This process consumes considerable time and energy and cannot be used for screening natural products that contain thermally unstable compounds. Therefore, an efficient analytical method is required. In this study, chitosan-based matrix solid-phase dispersion extraction was combined with cell membrane magnetic bead ligand-affinity assay to form a novel method for identifying the active compounds in Fructus Cnidii such as osthole and imperatorin. When compared with traditional cell membrane ligand-affinity assays, this assay requires less energy, extraction time (7 min), solvent volume (1.2 mL), and fewer natural products (40 mg). This indicates that the chitosan-based matrix solid-phase dispersion extraction assisted cell membrane magnetic beads ligand-affinity assay is an alternative analytical method for studying natural products.

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“…Conventional bio-activity guided separation has made great contribution to the discovery of bioactive compounds; however, it is cumbersome, high cost, and time consuming. Ligand fishing technology has been developed quickly in the past decade, which has exhibited great potential to improve the efficiency for discovery of bioactive compounds [15][16][17]. Target proteins have been immobilized on various solid supports including nanotubes [18], quantum dots [19], hollow fibers [20], and magnetic nanoparticles (MNPs) [21,22] to specifically extract their ligands from complex mixture based on the specific protein-ligand interaction.…”

mentioning

confidence: 99%

Tyrosinase covalently immobilized on carboxyl functionalized magnetic nanoparticles for fishing of the enzyme's ligands from Prunellae Spica

Yan

Bai

et al. 2022

J of Separation Science

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In this study, tyrosinase was immobilized on carboxyl functionalized silicacoated magnetic nanoparticles for the first time to be used for fishing of tyrosinase's ligands present in complex plant extract. The immobilized tyrosinase was characterized by transmission electron microscopy, vibrating sample magnetometry, Fourier transform infrared spectroscopy, thermo-gravimetric analyzer, and atomic force microscopy. The reusability and thermostability of the immobilized tyrosinase were found significantly superior to its free counterpart. Two tyrosinase's ligands, that is, caffeic acid (1) and rosmarinic acid (2), were fished out from extract of the traditional Chinese medicine Prunellae Spica by the immobilized tyrosinase. Compound 1 was found to be an activator of the enzyme with the half maximal effective concentration value of 0.27 ± 0.06 mM, while compound 2 was an inhibitor with the half maximal inhibitory concentration value of 0.14 ± 0.03 mM. Taking advantage of the convenience of magnetic separation and specific extraction ability of ligand fishing, the proposed method exhibited great potential for screening of bioactive compounds from complex matrices.

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Ligand fishing via a monolithic column coated with white blood cell membranes: A useful technique for screening active compounds in Astractylodes lancea

Cited by 6 publications

References 56 publications

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

Chitosan‐based matrix solid‐phase dispersion extraction assisted cell membrane magnetic bead ligand‐affinity assay for screening active compounds from Fructus Cnidii

Tyrosinase covalently immobilized on carboxyl functionalized magnetic nanoparticles for fishing of the enzyme's ligands from Prunellae Spica

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