Light and electron microscopic immunocytochemistry of neurotensin-like immunoreactive neurons in the rat hypothalamus

Ibata, Yasuhiko; Kawakami, Fumio; Fukui, Kenji; Obata-Tsuto, H.L.; Tanaka, Masaki; Kubo, Toshikazu; Okamura, Hitoshi; Morimoto, Nobumitsu; Yanaihara, Chizuko; Yanaihara, Noboru

doi:10.1016/0006-8993(84)90234-8

Cited by 54 publications

(12 citation statements)

References 19 publications

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“…Both POMC/CART-and NPY/AgRP-containing neurons, in turn, project to other hypothalamic regions, including VMH, DMH, LHA, and the paraventricular nucleus (PVN) to regulate food intake and energy balance (Chiesi et al, 2001;Barsh and Schwartz, 2002;Berthoud, 2002;Funahashi et al, 2003). In the rat hypothalamus, NT neurons are widely distributed in the ARC, PVN, DMH, and LHA as immunocytochemistry studies have revealed (Kahn et al, 1980;Ibata et al, 1984). Leptin receptors (ObR b ) are also widely distributed in the ARC, PVN, and LHA (Mercer et al, 1996;Hakansson et al, 1998;Baskin et al, 1999a,b;Elmquist et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Anorexigenic Hormones Leptin, Insulin, and α-Melanocyte-Stimulating Hormone Directly Induce Neurotensin (NT) Gene Expression in Novel NT-Expressing Cell Models

Cui¹,

Cai²,

Belsham³

2005

J. Neurosci.

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Section: Discussionmentioning

confidence: 99%

Anorexigenic Hormones Leptin, Insulin, and α-Melanocyte-Stimulating Hormone Directly Induce Neurotensin (NT) Gene Expression in Novel NT-Expressing Cell Models

Cui¹,

Cai²,

Belsham³

2005

J. Neurosci.

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“…All animals were perfused under sodium pentobarbital anesthesia with a fixative consisting of 4% paraformaldehyde and 0.2% picric acid in 0.1 Ai phosphate buffer (pH 7.4) as described previously [9]. Serial coronal frozen sections (20 pm in thickness) were incubated with either GRF antiserum (dilution 1:4.000) or SR1F antiserum (dilution 1:4,000) for 5 days at 4 C. Then the sections were proces sed for immunocytochemistry by a modified PAP method [9]. After the DAB reaction, staining intensities and distribution pattern of immunoreactive neurons and fibers in serial sections were first ex amined by light microscopy.…”

Section: Materials and Vlethodsmentioning

confidence: 99%

Age-Related Changes in Growth Hormone Releasing Factor and Somatostatin in the Rat Hypothalamus

Morimoto¹,

Kawakami

Makino³

et al. 1988

Neuroendocrinology

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Distribution and staining intensities of growth hormone releasing factor (GRF) and somatostatin (SRIF) were examined in young (3 months of age) and old (24 months of age) male rats of Sprague-Dawley strain, using the PAP immunocytochemical procedure. Some animals of each age group were intraventricularly injected with colchicine to demonstrate immunoreactive neuronal perikarya. GRF-immunoreactive intensities of old rats were markedly reduced in the median eminence as compared with those of young rats. No remarkable difference could be detected between SRIF immunoreactivities in the young and old animals, since intensive SRIF immunoreactivities were found in the external layer of the median eminence of both groups of animals. Between two age groups injected with colchicine, we also found no difference in the distribution and staining intensities of immunoreactive perikarya of GRF and SRIF in the hypothalamus and also detected no significant difference in total neuron numbers of each peptide. These findings suggest that the synthesis and/or release of GRF in GRF-containing neurons are decreased, though GRF-containing neurons themselves remain alive and have the capacity to synthesize GRF.

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“…In many vertebrates, NT is found in mucosal endo crine cells of the small intestine and in neurons of the central and peripheral nervous system [7]. Within the cen tral nervous system, immunocytochemical investigations have shown that NT is mainly distributed in the hypothal amus, notably in the paraventricular and periventricular cell groups, in the lateral hypothalamus [8][9][10] and in the median eminence [8,10,11]. In addition, NTimmunoreactivity has been observed in the pituitary gland by means of different techniques including RIA [12,13], HPLC [14] and immunohistochemistry [14][15][16].…”

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confidence: 99%

Identification of Neurotensin-lmmunoreactive Cells in the Anterior Pituitary of Normal and Castrated Rats

Bello

Dubourg²,

Kah³

et al. 1992

Neuroendocrinology

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The localization of neurotensin (NT) has been studied in the rat pituitary by means of immunocytochemistry at the light- and electron-microscopic levels. Cells immunoreactive for NT (NT-IR) were observed in the anterior lobe of rats of both sexes. Following an elution-restaining procedure, it was found that most of these NT-IR cells correspond to cells also reacting to β-LH or FSH antisera. However, a few NT-IR cells did not stain for β-LH nor FSH, but for TSH. The subcellular localization of NT was studied at the electron-microscopic level by means of the immunogold procedure. The immunoreactivity was always localized over the secretory granules. By using two sizes of gold particles, it was shown that NT- and β-LH-IR were colocalized in part of the cell granules. Castration caused a progressive decrease of the NT-IR in the β-LH- or FSH-positive cells, while the number of NR-IR, TSH-IR cells increased. After 2 weeks, NT-IR was mainly observed in this latter cell type. The present work demonstrates that NT-IR is localized within the secretory ganules of the gonadotrophs and a few thyrotrophs, indicating that NT could be released together with gonadotrophins and TSH. Moreover, since gonad-ectomy results in typical qualitative and quantitative changes of NT-IR gonadotrophs, the intervention of the pituitary NT in the regulation of the hypo-physogonadal axis is strongly suggested.

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Light and electron microscopic immunocytochemistry of neurotensin-like immunoreactive neurons in the rat hypothalamus

Cited by 54 publications

References 19 publications

Anorexigenic Hormones Leptin, Insulin, and α-Melanocyte-Stimulating Hormone Directly Induce Neurotensin (NT) Gene Expression in Novel NT-Expressing Cell Models

Anorexigenic Hormones Leptin, Insulin, and α-Melanocyte-Stimulating Hormone Directly Induce Neurotensin (NT) Gene Expression in Novel NT-Expressing Cell Models

Age-Related Changes in Growth Hormone Releasing Factor and Somatostatin in the Rat Hypothalamus

Identification of Neurotensin-lmmunoreactive Cells in the Anterior Pituitary of Normal and Castrated Rats

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