Light at night affects gut microbial community and negatively impacts host physiology in diurnal animals: Evidence from captive zebra finches

Malik, Indu; Batra, Twinkle; Das, Subhajit; Kumar, Vinod

doi:10.1016/j.micres.2020.126597

Cited by 16 publications

(5 citation statements)

References 63 publications

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“…After sequencing, the processing of sequenced data was implemented by the QIIME (V 1.8.0) software. After the filtration of low-quality sequences defined by Gill et al (2006) and Chen and Jiang (2014) and meaningless sequences (Including adapters, chimera, poly_A and primer), valid sequences were clustered into operational taxonomic units (OTUs) at a 97% similarity threshold via the UCLUST ( Malik et al, 2020 ). The Basic Local Alignment Search Tool was used for further taxonomic classification, and an OTU table was obtained ( Urbanek et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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This study was conducted to investigate the effects of different additives on the fermentation quality, nutrient composition, bacterial communities, and metabolic profiles of the silage of hybrid Pennisetum. The experiment was conducted using five treatments, i.e., CK, control group, MA, 1% malic acid of fresh matter (FM) basis, GL, 1% glucose of FM basis, CE, 100 U/g FM cellulase, and BS, 106 cfu/g FM Bacillus subtilis, with six replicates each treatment. After a 120-day fermentation, 30 silage packages were opened for subsequent determination. As a result, all four additives had positive effects on the fermentation quality and nutrient composition of the silage of hybrid Pennisetum. The high-throughput sequencing of V3–V4 regions in 16S rRNA was performed, and results showed that Firmicutes and Proteobacteria were the dominant phyla and that Aquabacterium and Bacillus were the dominant genera. MA, GL, CE, and BS treatment resulted in 129, 21, 25, and 40 differential bacteria, respectively. The four additives upregulated Bacillus smithii but downregulated Lactobacillus rossiae. Metabolic profiles were determined by UHPLC-Q/TOF-MS technology and the differential metabolites caused by the four additives were 47, 13, 47, and 18, respectively. These metabolites played antioxidant, antibacterial, and anti-inflammatory functions and involved in pathways, such as the citrate cycle, carbon fixation in photosynthetic organisms, and glyoxylate and dicarboxylate metabolism. In conclusion, silage additives promoted fermentation quality and nutrient composition by altering bacterial communities and metabolic profiles. This study provided potential biomarkers for the improvement of silage quality.

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Section: Methodsmentioning

confidence: 99%

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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“…After high-throughput sequencing, QIIME (V 1.8.0) was adopted for the processing of the sequenced data. After the filtration of chimera and low-quality sequences described by previous reports [ 25 , 26 ], the effective sequences were obtained and clustered into operational taxonomic units (OTUs) via UCLUST with a 97% similarity threshold [ 27 ]. A representative sequence from each OTU was selected for further taxonomic classification conducted via the Basic Local Alignment Search Tool (BLAST).…”

Section: Methodsmentioning

confidence: 99%

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

et al. 2021

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The present study investigated the effects of malic acid, sucrose, and their mixture on the fermentation parameters, Cornell Net Carbohydrate and Protein System (CNCPS) nitrogen fractions, and bacterial community of Moringa oleifera leaves (MOL) silages. The trial was divided into four treatments and labeled as CON (control group) and MLA, SUC, and MIX (respectively denoting the addition of 1% malic acid, 1% sucrose, and 1% malic acid + 1% sucrose to the fresh weight basis). The silage packages were opened on the 2nd, 5th, 10th, 20th, and 40th days of ensiling for subsequent determination. Malic acid and sucrose increased the lactic acid content (p < 0.05) and pH value, and the acetic acid contents of MLA and MIX were lower than those in CON (p < 0.05). Compared with sucrose, malic acid had a better capacity to preserve nutrients and inhibit proteolysis, and thus exerted better effects on the CNCPS nitrogen fractions. The results of 16S rRNA showed that the dominant phyla were Firmicutes and Proteobacteria and that the dominant genera were Lactobacillus and Weissella. With the application of silage additives and the processing of fermentation, there was a remarkable change in the composition and function of the bacterial community. The variation of the fermentation parameters and CNCPS nitrogen fractions in the MOL silages caused by malic acid and sucrose might be attributed to the dynamic and dramatic changes of the bacterial community.

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“…Additionally, dLAN exposure abolished the rhythm in expression of clock and cry1 genes [26]. Further, ALAN impairs metabolism and alters metabolic gene (sirtuin1; sirt1, glucose 6-phosphatase; g6pc and Forkhead box protein O1; foxo1) expression in Zebra Finches [29,30]. Another study demonstrated the effects on diurnal pattern of gene expression of pro-inflammatory (interleukin-1β; IL-1β, interleukin-6; IL-6) and antiinflammatory (IL-10) genes in the brain of Zebra Finches [31].…”

Section: Artificial Light At Night-circadian Misalignment and Sleep D...mentioning

confidence: 98%

Artificial Light at Night, Higher Brain Functions and Associated Neuronal Changes: An Avian Perspective

Taufique

2022

Birds

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In recent times, there has been an unprecedented increase in usage of electrical lightning. This has led to increase in artificial light at night (ALAN), and it has been suggested as a source of environmental pollution. ALAN exposure has been reported to be associated with disruption of daily rhythms and serious health consequences, such as immune, metabolic, and cognitive dysfunctions in both birds and mammals. Given the worldwide pervasiveness of ALAN, this research topic is also important from an ecological perspective. In birds, daily timings and appropriate temporal niches are important for fitness and survival. Daily rhythms in a wide array of functions are regulated by the circadian clock(s) and endogenous oscillators present in the body. There is accumulating evidence that exposure to ALAN disrupts clock-regulated daily rhythms and suppresses melatonin and sleep in birds. Circadian clock, melatonin, and sleep regulate avian cognitive performance. However, there is limited research on this topic, and most of the insights on the adverse effects of ALAN on cognitive functions are from behavioural studies. Nevertheless, these results raise an intriguing question about the molecular underpinning of the ALAN-induced negative consequences on brain functions. Further research should be focused on the molecular links between ALAN and cognitive performance, including the role of melatonin, which could shed light on the mechanism by which ALAN exposures lead to negative consequences.

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Light at night affects gut microbial community and negatively impacts host physiology in diurnal animals: Evidence from captive zebra finches

Cited by 16 publications

References 63 publications

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

Artificial Light at Night, Higher Brain Functions and Associated Neuronal Changes: An Avian Perspective

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