Light-induced cytotoxicity in cells utilizing an automated well plate illuminator

Abstract

Delivering therapeutic drug molecules to the target site and releasing the cargo site-specifically is of major interest in biomedicine. To carry and release drugs to specific target tissues, different nanotechnology approaches have been utilized. These include light-sensitive liposomal carriers, which have been engineered to release cargo from their aqueous cores when illuminated by certain wavelengths of laser light. To study drug release parameters in vitro, Modulight has designed and automated biomedical illumination system ML8500. ML8500 can be tailored to house up to eight Modulight semiconductor lasers ranging from 400nm-2000nm selected based on the optical properties of dyes and molecules of interest. The illumination system can be configured for different well plate types and includes environmental control of temperature and CO2 to provide stable conditions for the studied cell types. Utilizing the ML8500 illumination system, the safety of laser light illumination for the liposomal drug delivery was investigated in retinal pigment epithelial (ARPE-19) cell line.

In Vitro Antimicrobial Photodynamic Therapy for Pseudomonas aeruginosa (P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA) Inhibition Using a Green Light Source

“…Currently, it is common in PDT lighting sources for in vitro experiments to use LED technology because of its low cost and relative ease of implementation. Besides this peculiarity, a greater number of lighting sources are usually developed for the implementation of aPDT in multiwell cell culture plates [31][32][33][34][35] and even for vials [32], which could increase the cost of experimentation by requiring a greater amount of materials or supplies during the studies [36].…”

“…For this reason, a better option is usually the use of LED lighting sources, as they are less expensive, cause less of a thermal effect, and allow easy adaptation in matrix configurations for cell culture dishes [29][30][31]. Some lab companies have developed light sources with this configuration [32]. Occasionally, researchers fabricate their own illumination sources for the irradiation of cell culture wells; however, they do not consistently utilize all wells, as the emitted light from one may interfere with neighboring elements.…”

“…On the other hand, in vitro experiments could be run using well plates, test tubes, and even vials, depending on how the experiment was set up. This has led to the development of lighting sources for PDT that allow direct irradiation of samples in test tubes or in vials [32].…”