Light-induced infrared difference spectroscopy on three different forms of orange carotenoid protein: focus on carotenoid vibrations

Leccese, Silvia; Wilson, Adjélé; Kirilovsky, Diana; Spezia, Riccardo; Jolivalt, Claude; Mezzetti, Alberto

doi:10.1007/s43630-023-00384-7

Cited by 7 publications

(1 citation statement)

References 37 publications

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“…(1) They can help us better understand the mechanism of photo-activation, which is under intensive study and the object of lively debate ( [3,[35][36][37][38][39] and refs. therein); (2) They provide new information concerning the influence of the surrounding environment on the orange to red activation;…”

Section: Discussionmentioning

confidence: 99%

Orange Carotenoid Protein in Mesoporous Silica: A New System towards the Development of Colorimetric and Fluorescent Sensors for pH and Temperature

Leccese,

Calcinoni,

Wilson

et al. 2023

Micromachines

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Orange carotenoid protein (OCP) is a photochromic carotenoprotein involved in the photoprotection of cyanobacteria. It is activated by blue-green light to a red form OCPR capable of dissipating the excess of energy of the cyanobacterial photosynthetic light-harvesting systems. Activation to OCPR can also be achieved in the dark. In the present work, activation by pH changes of two different OCPs—containing echinenone or canthaxanthin as carotenoids—is investigated in different conditions. A particular emphasis is put on OCP encapsulated in SBA-15 mesoporous silica nanoparticles. It is known that in these hybrid systems, under appropriate conditions, OCP remains photoactive. Here, we show that when immobilised in SBA-15, the OCP visible spectrum is sensitive to pH changes, but such a colorimetric response is very different from the one observed for OCP in solution. In both cases (SBA-15 matrices and solutions), pH-induced colour changes are related either by orange-to-red OCP activation, or by carotenoid loss from the denatured protein. Of particular interest is the response of OCP in SBA-15 matrices, where a sudden change in the Vis absorption spectrum and in colour is observed for pH changing from 2 to 3 (in the case of canthaxanthin-binding OCP in SBA-15: λMAX shifts from 454 to 508 nm) and for pH changing from 3 to 4 (in the case of echinenone-binding OCP in SBA-15: λMAX shifts from 445 to 505 nm). The effect of temperature on OCP absorption spectrum and colour (in SBA-15 matrices) has also been investigated and found to be highly dependent on the properties of the used mesoporous silica matrix. Finally, we also show that simultaneous encapsulation in selected surface-functionalised SBA-15 nanoparticles of appropriate fluorophores makes it possible to develop OCP-based pH-sensitive fluorescent systems. This work therefore represents a proof of principle that OCP immobilised in mesoporous silica is a promising system in the development of colorimetric and fluorometric pH and temperature sensors.

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