Light induces gene expression to enhance the synthesis of storage reserves in Brassica napus L. embryos

Tan, Honglue; Xiao, Qi; Li, Yán; Wang, Xingchun; Zhou, Jianguo; Liu, Xiuhong; Shi, Xiaoli; Ye, Wenxue; Xiang, Xiaoe

doi:10.1007/s11103-020-01003-0

Cited by 6 publications

(13 citation statements)

References 45 publications

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“…Obviously, the mature seeds of GH06 and ZY821 under treatment were light yellow and brown in color and wrinkled in shape as compared to healthy, round-shaped dark yellow and black seeds of control, respectively. Our findings indicate that light is an essential factor for the morphological development and biosynthesis of storage reserves in seeds, consistent with similar observations in B. napus (Tan et al, 2015(Tan et al, , 2020.…”

Section: Characterization Of Siliques Under Normal and Darknesssupporting

confidence: 92%

“…Our findings indicate that light is an essential factor for the morphological development and biosynthesis of storage reserves in seeds, consistent with similar observations in B . napus ( Tan et al, 2015 , 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Previously results showed that siliques are the dominant photosynthetic organ for supporting the seed filling during late stages (Tan et al, 2020). To elucidate the effects of light on the biosynthesis of storage reserves of siliques for contributing to seed development of B. napus, we analyzed the accumulation of metabolites in siliques under the control and shading treatment by UPLC-HESI-MS/MS with negative mode.…”

Section: Brassica Napusmentioning

confidence: 99%

“…For example, the timing, intensity, and duration of light treatment could be used as a parameter to measure the GSLs fluctuations during different developmental stages (Kirkegaard et al, 2018). In B. napus, silique walls are the dominant photosynthetic organ for supporting the seed filling during late stages (Tan et al, 2020), which were acted as one of the main parameters for yield determination. Previous studies showed that the siliques are also the main production sites for GSLs biosynthesis in rapeseed and which are later transported from silique walls (source) to the seeds (sink) (Du and Halkier, 1998;Jørgensen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

Kamal

Shen

et al. 2022

Front. Plant Sci.

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Glucosinolates (GSLs) are naturally occurring secondary metabolites found in the Brassicaceae family, which mainly synthesize in the siliques with a wide range of functions. In this study, we investigated the effects of lights on metabolites in siliques of rapeseed through ultra high-performance liquid chromatography (UPLC)—heated electrospray ionization (HESI)–tandem mass spectrometry (MS/MS). A total of 249 metabolites, including 29 phenolic acids, 38 flavonoids, 22 GSLs, 93 uncalculated and 67 unknown compounds, were identified in siliques of rapeseed. Meanwhile, 62 metabolites showed significant differences after shading treatment, which were mainly GSLs and unknown compounds. Interestingly, the amounts of 10 GSLs had high accumulation levels in siliques, while the expression levels of their corresponding biosynthetic genes (AOP, GSL-OH, IGMT, and ST5a) were obviously reduced after shading treatment. Further evidence showed that the amounts of GSLs were significantly reduced in seeds, in accordance with the expression profiles of transporter genes (BnaGTRs). Our findings indicated that lights could affect the accumulation and transportation of GSLs from siliques to seeds in rapeseed. Therefore, this study facilitates a better understanding of metabolic characteristics of siliques and provides insight into the importance of light for GSLs accumulation and transportation in siliques and seeds of rapeseed.

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Section: Characterization Of Siliques Under Normal and Darknesssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Brassica Napusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

Kamal

Shen

et al. 2022

Front. Plant Sci.

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The LEA2 gene sub-family: Characterization, evolution, and potential functions in Camellia oleifera seed development and stress response

Liu

Chen

et al. 2023

Scientia Horticulturae

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Triacylglycerol biosynthesis in shaded seeds of tung tree (Vernicia fordii) is regulated in part by Homeodomain Leucine Zipper 21

Zhang

et al. 2021

The Plant Journal

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Summary Light quantity and quality affect many aspects of plant growth and development. However, few reports have addressed the molecular connections between seed oil accumulation and light conditions, especially dense shade. Shade‐avoiding plants can redirect plant resources into extension growth at the expense of leaf and root expansion in an attempt to reach areas containing richer light. Here, we report that tung tree seed oil accumulation is suppressed by dense shade during the rapid oil accumulation phase. Transcriptome analysis confirmed that oil accumulation suppression due to dense shade was attributed to reduced expression of fatty acid and triacylglycerol biosynthesis‐related genes. Through weighted gene co‐expression network analysis, we identified 32 core transcription factors (TFs) specifically upregulated in densely shaded seeds during the rapid oil accumulation period. Among these, VfHB21, a class I homeodomain leucine zipper TF, was shown to suppress expression of FAD2 and FADX, two key genes related to α‐eleostearic acid, by directly binding to HD‐ZIP I/II motifs in their respective promoter regions. VfHB21 also binds to similar motifs in the promoters of VfWRI1 and VfDGAT2, two additional key seed lipid regulatory/biosynthetic genes. Functional conservation of HB21 during plant evolution was demonstrated by the fact that AtWRI1, AtSAD1, and AtFAD2 were downregulated in VfHB21‐overexpressor lines of transgenic Arabidopsis, with concomitant seed oil reduction, and the fact that AtHB21 expression also was induced by shade. This study reveals some of the regulatory mechanisms that specifically control tung tree seed oil biosynthesis and more broadly regulate plant storage carbon partitioning in response to dense shade conditions.

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Light induces gene expression to enhance the synthesis of storage reserves in Brassica napus L. embryos

Cited by 6 publications

References 45 publications

Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

Metabolite Characteristics Analysis of Siliques and Effects of Lights on the Accumulation of Glucosinolates in Siliques of Rapeseed

The LEA2 gene sub-family: Characterization, evolution, and potential functions in Camellia oleifera seed development and stress response

Triacylglycerol biosynthesis in shaded seeds of tung tree (Vernicia fordii) is regulated in part by Homeodomain Leucine Zipper 21

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