Light Intensity-Induced Changes in <i>cab</i> mRNA and Light Harvesting Complex II Apoprotein Levels in the Unicellular Chlorophyte <i>Dunaliella tertiolecta</i>

LaRoche, Julie; Mortain‐Bertrand, Anne; Falkowski, Paul G.

doi:10.1104/pp.97.1.147

Cited by 78 publications

(49 citation statements)

References 22 publications

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“…1). This result for E. huxleyi is consistent with results found for T. weissflogii (Post et al, 1984), D. tertiolecta (La Roche et al, 1991) and Skeletonema costatum where cellular photosynthetic pigment contents were found to be diluted by increased cell division rather than by degradation of pigments. Goericke & Welschmeyer (1992a) provided direct evidence for low chlorophyll turnover rates (50.1 d…”

Section: Rate Of Acclimation Of Cellular Chl a Contentsupporting

confidence: 90%

“…In this diatom, it took longer than four days for cellular chl a content to reach a new steady state value following transfer. In contrast, in the chlorophyte D. tertiolecta, cellular chl a content increased three fold, to a new steady state level within 18 h following transfer to low PFD (La Roche et al, 1991). Thus, there are significant interspecific differences in acclimation time scales amongst taxa, even though these taxa have similar light-saturated growth rates and were subjected to similar changes of PFD.…”

mentioning

confidence: 92%

“…It has been a long-standing goal of research in phytoplankton ecophysiology to be able to describe and predict the response of phytoplankton to PFD fluctuations (Cullen & Lewis, 1988). A first step in this process is to examine the changes in photosynthetic performance, pigment and biomass synthesis, and cell division following step changes in PFD (Cullen & Lewis, 1988;La Roche et al, 1991;Anning et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…The rate of pigment synthesis also changes rapidly, leading to longterm changes in pigment content and composition Correspondence to: Richard J. Geider. E-mail: geider@ essex.ac.uk (Post et al, 1984;La Roche et al, 1991;Anning et al, 2000). The rate of change of these physiological responses is species specific and tends to be faster upon shifts from low to high PFD than upon shifts from high to low PFD.…”

Section: Introductionmentioning

confidence: 99%

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Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Harris

Scanlan

Geider

2009

European Journal of Phycology

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A non-calcifying strain (Ply 92) of Emiliania huxleyi (Lohman) Hay et Mohler, was subjected to reciprocal step changes of photon flux density (PFD) between 50 and 800 mmol photons m À2 s À1 to establish the timescales of photoacclimation.Photoacclimation in E. huxleyi involved adjustment of cellular light harvesting pigment contents, but not cellular ribulose bisphosphate carboxylase-oxygenase (RuBisCO) or cellular RNA contents. There was considerable variability in both the magnitude and the rate of the response of physiological and biophysical variables to the step changes in PFD. The slowest response was observed in cellular chl a content, and the chl a/carbon ratio. Acclimation of these variables appeared to be due to dilution of pigment pools rather than to pigment turnover. Changes in the chl a-specific light absorption coefficient accompanied changes in cellular chl a content. Following the shifts in PFD, the efficiency of excitation energy transfer from the photosystem II antennae to reaction centres II (RCII), as assessed from F Changes in non-photochemical excitation energy quenching were correlated with the relative and absolute abundance of the xanthophyll cycle pigments diadinoxanthin and diatoxanthin, which appeared to be reversibly interconverted with fucoxanthin. Thus, acclimation to PFD involved co-ordinated adjustment of the quantum efficiency of RCII, the efficiency of excitation energy transfer from the light-harvesting pigment bed to RCII, and cellular light absorption on overlapping time scales.

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Section: Rate Of Acclimation Of Cellular Chl a Contentsupporting

confidence: 90%

mentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Harris

Scanlan

Geider

2009

European Journal of Phycology

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“…However, several studies, based on microcosm experiments, have been performed to better understand the influence of atmospheric dust deposition on the functioning and productivity in low-nutrient low-chlorophyll (LNLC) environments, either in the Atlantic Ocean (Blain et al, 2004;Mills et al, 2004;Moore et al, 2008;Marañón et al, 2010), Pacific Ocean (Law et al, 2011), or Mediterranean Sea (e.g., Pulido-Villena et al, 2008;Wagener et al, 2010).…”

Section: Giovagnetti Et Al: Assessing the Role Of Dust Depositionmentioning

confidence: 99%

Assessing the role of dust deposition on phytoplankton ecophysiology and succession in a low-nutrient low-chlorophyll ecosystem: a mesocosm experiment in the Mediterranean Sea

Giovagnetti

Brunet²,

Conversano³

et al. 2013

Biogeosciences

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In this study, we investigate the response of the phytoplankton community, with emphasis on ecophysiology and succession, after two experimental additions of Saharan dust in the surface water layer of a low-nutrient low-chlorophyll ecosystem in the Mediterranean Sea. Three mesocosms were amended with evapocondensed dust to simulate realistic Saharan dust events, while three additional mesocosms were kept unamended and served as controls. The experiment consisted in two consecutive dust additions and samples were daily collected at different depths (−0.1, −5 and −10 m) during one week, starting before each addition occurred. Data concerning HPLC pigment analysis on two size classes (< 3 and > 3 μm), electron transport rate (ETR) vs. irradiance curves, non-photochemical fluorescence quenching (NPQ) and phytoplankton cell abundance (measured by flow cytometry), are presented and discussed in this paper. Results show that picophytoplankton mainly respond to the first dust addition, while the second addition leads to an increase of both pico- and nano-/microphytoplankton. Ecophysiological changes in the phytoplankton community occur, with NPQ and pigment concentration per cell increasing after dust additions. While biomass increases after pulses of new nutrients, ETR does not greatly vary between dust-amended and control conditions, in relation with ecophysiological changes within the phytoplankton community, such as the increase in NPQ and pigment cellular concentration. A quantitative assessment and parameterisation of the onset of a phytoplankton bloom in a nutrient-limited ecosystem is attempted on the basis of the increase in phytoplankton biomass observed during the experiment. The results of this study are discussed focusing on the adaptation of picophytoplankton to nutrient limitation in the surface water layer, as well as on size-dependent competition ability in phytoplankton

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Rapid photoacclimation during episodic deep mixing augments the biological carbon pump

Penta

Fox

Halsey

2021

Limnology & Oceanography

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Episodic deep mixing events are one component of the biological carbon pump that physically transports organic carbon into the mesopelagic. Episodic deep mixing also disrupts summertime thermal stratification thereby changing the light field and nutrient concentrations available for phytoplankton growth. Phytoplankton survival and growth below the mixed layer following restratification depends on how rapidly cells can employ a variety of photoacclimation processes in response to the environmental changes. To compare the relative timescales of summertime episodic deep mixing events with the timescales of phytoplankton photoacclimation processes, we first analyzed autonomous float data to survey the frequency and magnitude of deep mixing events in the western North Atlantic Ocean. Next, we simulated a sustained deep mixing event in the laboratory and measured rates of acclimation processes ranging from light harvesting to growth in a model diatom and green alga. In both algae increases in chlorophyll (Chl) were coupled to growth, but growth of the green alga lagged the diatom by about a day. In float profiles, significant increases in Chl and phytoplankton carbon (C phyto ) were detected below the mixed layer following episodic deep mixing events. These events pose a previously unrecognized source of new production below the mixed layer that can significantly boost the amount of carbon available for export to the deep ocean.

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Light Intensity-Induced Changes in cab mRNA and Light Harvesting Complex II Apoprotein Levels in the Unicellular Chlorophyte Dunaliella tertiolecta

Cited by 78 publications

References 22 publications

Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Responses ofEmiliania huxleyi(Prymnesiophyceae) to step changes in photon flux density

Assessing the role of dust deposition on phytoplankton ecophysiology and succession in a low-nutrient low-chlorophyll ecosystem: a mesocosm experiment in the Mediterranean Sea

Rapid photoacclimation during episodic deep mixing augments the biological carbon pump

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